Spodoptera littoralis (Lepidoptera: Noctuidae) Resistance to CryIC and Cross-Resistance to Other Bacillus thuringiensis Crystal Toxins

1996 ◽  
Vol 89 (4) ◽  
pp. 791-797 ◽  
Author(s):  
Judy Müller-Cohn ◽  
Josette Chaufaux ◽  
Christophe Buisson ◽  
Nathalie Gilois ◽  
Vincent Sanchis ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Spodoptera Littoralis ◽  
Cross Resistance ◽  
Crystal Toxins ◽  
Lepidoptera Noctuidae

scholarly journals Combinational Effect of Rumex tingitanus (Polygonaceae) Hexane Extract and Bacillus thuringiensis  δ-Endotoxin against Spodoptera littoralis (Lepidoptera: Noctuidae)

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Dhekra Mhalla ◽  
Dalel Ben Farhat-Touzri ◽  
Slim Tounsi ◽  
Mohamed Trigui
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Bacillus Thuringiensis ◽  
Synergistic Effect ◽  
Spodoptera Littoralis ◽  
Insect Pests ◽  
Hexane Extract ◽  
Active Fraction ◽  
Lepidoptera Noctuidae ◽  
Potential Use

The increasing insect resistance against Bacillus thuringiensis delta-endotoxins is a serious problem which makes it urgent to look for new eco-friendly strategies. Combining these toxins with other biomolecules is one of the promising strategies against insect pests. In this work, we evaluated the bioinsecticidal potential of Rumex tingitanus extracts and B. thuringiensis strain BLB250 against Spodoptera littoralis (Lepidoptera: Noctuidae) larvae. The chemical composition of the hexane extract, the most active fraction, was analyzed to validate the correlation between chemical composition and biological activity. Among the tested extracts, only the hexanic extract showed toxicity against first and second instar larvae with LC50 of 2.56 and 2.95 mg g−1, respectively. The Bacillus thuringiensis BLB250 delta-endotoxins showed toxicity with an LC50 of 56.3 μg g−1. Therefore, the investigated combinational effect of BLB250 delta-endotoxins and R. tingitanus hexane extract proved significant synergistic effect against S. littoralis larvae. The GC-MS analysis of R. tingitanus hexane extract showed the richness of this extract in phytosterols such as β and γ-sitosterol (48.91%), campesterol (6.43%), and β-amyrin (8.92%) which are known for their insecticidal activity. This novel finding highlights the potential use of this combination against insect pests to prevent the appearance of resistance problems.

Inheritance of Resistance to the Bacillus thuringiensis CrylC Toxin in Spodoptera littoralis (Lepidoptera: Noctuidae)

1997 ◽  
Vol 90 (4) ◽  
pp. 873-878 ◽  
Author(s):  
Josette Chaufaux ◽  
Judy MÛller-Cohn ◽  
Christophe Buisson ◽  
Vincent Sanchis ◽  
Didier Lereclus ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Spodoptera Littoralis ◽  
Inheritance Of Resistance ◽  
Lepidoptera Noctuidae

Inheritance of Cry1F resistance, cross-resistance and frequency of resistant alleles inSpodoptera frugiperda(Lepidoptera: Noctuidae)

2013 ◽  
Vol 103 (6) ◽  
pp. 700-713 ◽  
Author(s):  
A.M. Vélez ◽  
T.A. Spencer ◽  
A.P. Alves ◽  
D. Moellenbeck ◽  
R.L. Meagher ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Bacillus Thuringiensis ◽  
Spodoptera Frugiperda ◽  
Resistant Strain ◽  
Parental Strain ◽  
Single Locus ◽  
Cross Resistance ◽  
Screening Assay ◽  
Low Levels ◽  
Lepidoptera Noctuidae

AbstractTransgenic maize,Zea maizeL., expressing the Cry1F protein fromBacillus thuringiensishas been registered forSpodoptera frugiperda(J. E. Smith) control since 2003. Unexpected damage to Cry1F maize was reported in 2006 in Puerto Rico and Cry1F resistance inS. frugiperdawas documented. The inheritance of Cry1F resistance was characterized in aS. frugiperdaresistant strain originating from Puerto Rico, which displayed >289-fold resistance to purified Cry1F. Concentration–response bioassays of reciprocal crosses of resistant and susceptible parental populations indicated that resistance is recessive and autosomal. Bioassays of the backcross of the F1generation crossed with the resistant parental strain suggest that a single locus is responsible for resistance. In addition, cross-resistance to Cry1Aa, Cry1Ab, Cry1Ac, Cry1Ba, Cry2Aa and Vip3Aa was assessed in the Cry1F-resistant strain. There was no significant cross-resistance to Cry1Aa, Cry1Ba and Cry2Aa, although only limited effects were observed in the susceptible strain. Vip3Aa was highly effective against susceptible and resistant insects indicating no cross-resistance with Cry1F. In contrast, low levels of cross-resistance were observed for both Cry1Ab and Cry1Ac. Because the resistance is recessive and conferred by a single locus, an F1screening assay was used to measure the frequency of Cry1F-resistant alleles from populations of Florida and Texas in 2010 and 2011. A total frequency of resistant alleles of 0.13 and 0.02 was found for Florida and Texas populations, respectively, indicating resistant alleles could be found in US populations, although there have been no reports of reduced efficacy of Cry1F-expressing plants.

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