Cyromazine Resistance in Drosophila melanogaster (Diptera: Drosophilidae) Generated by Ethyl Methanesulfonate Mutagenesis

1993 ◽  
Vol 86 (4) ◽  
pp. 1001-1008 ◽  
Author(s):  
Greg J. Adcock ◽  
Philip Batterham ◽  
Leonard E. Kelly ◽  
John A. McKenzie
Keyword(s):  
Drosophila Melanogaster ◽  
Ethyl Methanesulfonate ◽  
Ethyl Methanesulfonate Mutagenesis

Whole-Genome Effects of Ethyl Methanesulfonate-Induced Mutation on Nine Quantitative Traits in Outbred Drosophila melanogaster

Genetics ◽  
2001 ◽  
Vol 157 (3) ◽  
pp. 1257-1265 ◽  
Author(s):  
Hsiao-Pei Yang ◽  
Ana Y Tanikawa ◽  
Wayne A Van Voorhies ◽  
Joana C Silva ◽  
Alexey S Kondrashov
Keyword(s):  
Drosophila Melanogaster ◽  
Quantitative Traits ◽  
Spontaneous Mutation ◽  
Developmental Time ◽  
Ethyl Methanesulfonate ◽  
Induced Mutations ◽  
Mean Values ◽  
Eye Color ◽  
Recessive Mutations ◽  
The Mean

Abstract We induced mutations in Drosophila melanogaster males by treating them with 21.2 mm ethyl methanesulfonate (EMS). Nine quantitative traits (developmental time, viability, fecundity, longevity, metabolic rate, motility, body weight, and abdominal and sternopleural bristle numbers) were measured in outbred heterozygous F3 (viability) or F2 (all other traits) offspring from the treated males. The mean values of the first four traits, which are all directly related to the life history, were substantially affected by EMS mutagenesis: the developmental time increased while viability, fecundity, and longevity declined. In contrast, the mean values of the other five traits were not significantly affected. Rates of recessive X-linked lethals and of recessive mutations at several loci affecting eye color imply that our EMS treatment was equivalent to ∼100 generations of spontaneous mutation. If so, our data imply that one generation of spontaneous mutation increases the developmental time by 0.09% at 20° and by 0.04% at 25°, and reduces viability under harsh conditions, fecundity, and longevity by 1.35, 0.21, and 0.08%, respectively. Comparison of flies with none, one, and two grandfathers (or greatgrandfathers, in the case of viability) treated with EMS did not reveal any significant epistasis among the induced mutations.

scholarly journals TEMPERATURE-SENSITIVE MUTATIONS IN DROSOPHILA MELANOGASTER. XVII. HEAT- AND COLD-SENSITIVE LETHALS ON CHROMOSOME 3

Genetics ◽  
1973 ◽  
Vol 74 (3) ◽  
pp. 509-520
Author(s):  
S Elaine Tasaka ◽  
David T Suzuki
Keyword(s):  
Drosophila Melanogaster ◽  
Ethyl Methanesulfonate ◽  
Cold Sensitivity ◽  
Chromosome 3 ◽  
Temperature Sensitive ◽  
Single Mutation ◽  
Physical Length ◽  
Lethal Mutations ◽  
Genetic Length ◽  
Cold Sensitive

ABSTRACT Ethyl methanesulfonate-treated third chromosome of Drosophila melanogaster were tested for the presence of dominant and recessive temperature-sensitive lethal mutations at 17°, 22° and 29°C. Out of 1,176 chromosomes tested, no dominant ts lethals, 21 heat-sensitive, 22 cold-sensitive and 10 heat-cold-sensitive lethals were recovered. Heat-cold sensitivity was produced by a single mutation in all cases. Sixty-two percent of the ts lethals were fertile as homozygotes in both sexes. Surprisingly, 88% of the ts lethals mapped between st and Sb, a region straddling the centromere and estimated to comprise 12.9% of the genetic length and 55% of the physical length of chromosome 3. All but one of the heat- and cold-sensitive lethals complemented with each other at their respective restrictive temperatures.

Mutational specificity of ethyl methanesulfonate in excision-repair-proficient and -deficient strains of Drosophila melanogaster

1991 ◽  
Vol 229 (2) ◽  
pp. 213-218 ◽  
Author(s):  
Albert Pastink ◽  
Evert Heemskerk ◽  
Madeleine J.M. Nivard ◽  
Cees J. van Vliet ◽  
Ekkehart W. Vogel
Keyword(s):  
Drosophila Melanogaster ◽  
Excision Repair ◽  
Ethyl Methanesulfonate ◽  
Mutational Specificity

scholarly journals Molecular localization and developmental expression of the otu locus of Drosophila melanogaster

1988 ◽  
Vol 8 (4) ◽  
pp. 1481-1488
Author(s):  
P K Mulligan ◽  
J D Mohler ◽  
L J Kalfayan
Keyword(s):  
Drosophila Melanogaster ◽  
Restriction Fragment ◽  
Molecular Level ◽  
Ethyl Methanesulfonate ◽  
Developmental Expression ◽  
Restriction Fragments ◽  
Rna Probes ◽  
A Minor ◽  
Derivatives Of ◽  
Tumor Gene

The female-sterile ovarian tumor gene, otu, is located in cytological region 7F1 on the Drosophila melanogaster chromosome map. We have mapped the gene at the molecular level by using four dysgenic alleles and two revertant derivatives of these alleles as well as an ethyl methanesulfonate-induced allele. The insertional (dysgenic) changes were all associated with one restriction fragment, and its size was restored after phenotypic reversion. One ethyl methanesulfonate-induced allele had a deletion in the restriction fragment adjacent (distal) to the fragment altered in the insertional alleles. These two restriction fragments were immediately adjacent to the s38 chorion gene. Associated with the two altered restriction fragments were two RNA species, an abundant 3.2-kilobase (kb) poly(A)+ RNA and a minor 4.0-kb RNA. Several other less-abundant RNA species were detectable with more-sensitive single-stranded RNA probes. The otu gene was transcribed proximal to distal relative to the centromere; this was opposite to the direction of transcription of the adjacent s38 gene. During development, the 3.2-kb RNA was absent in larvae, first appeared in the pupal stages, and persisted in adult females, in which it was most prevalent in the ovaries. The DNA that hybridized to the 3.2-kb ovarian RNA hybridized to four different RNAs found in the testes but not in the rest of the adult male. These testis-enriched RNAs were transcribed from the same strand of DNA as the ovarian transcripts.

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