Reexamination of the Female Sex Pheromone of the Peach Twig Borer: Field Screening of Minor Constituents of Pheromone Gland Extracts and of Pheromone Analogs

1992 ◽  
Vol 85 (5) ◽  
pp. 1709-1716 ◽  
Author(s):  
Jocelyn G. Millar ◽  
Richard E. Rice
2003 ◽  
Vol 127 (3) ◽  
pp. 121-126 ◽  
Author(s):  
G. Raspotnig ◽  
R. Schicho ◽  
E. Stabentheiner ◽  
C. Magnes ◽  
M. Stelzl

1976 ◽  
Vol 66 (3) ◽  
pp. 427-435 ◽  
Author(s):  
R. J. Marks

AbstractThe four components of the synthesised sex pheromone of Diparopsis castanea Hmps. are dodecan-1-yl acetate (I); trans-9-dodecen-1-yl acetate (IIA); 11-dodecen-l-yl acetate (IIB); and trans-9,ll-dodecadien-l-yl acetate (IV). Increasing the proportion of IIA in a I/IIA/IIB/IV mixture progressively decreased male excitation in a laboratory bioassay, whilst in the absence of I all concentrations of IIA tested were equally inhibitory. The synthetic combination of 80% IV (93% trans: 7% cis) and 20% IIB (=dicastalure), was 485 times more potent in eliciting male activity than a female sex pheromone gland extract. Reduction in male response to crude extract occurred through exposure of males to glandular IIA and through reduced pheromone volatility caused by extracted triglycerides. Maximum quantities of pheromone were detected in the female sex pheromone gland 6–12 h into scotophase and for at least 30 min into photophase. Significantly greater bioassay responses were elicited by the optimal field combination of 80% IV and 20% IIB than for other ratios tested. Excitation and clasper extension were observed in response to the non-attractive IIB moiety and this indicates a role in close-range mating behaviour for this component. The threshold level of male response was estimated to occur in response to sex pheromone at an aerial concentration of 9·2 molecules IV mm3/s−1. This suggests that aerial concentrations of 102–103 times the male threshold response level may be sufficient to cause successful communication disruption in the field. The problems of relating the non-oriented responses in an assay of this type to directional responses in traps are briefly discussed.


1993 ◽  
Vol 19 (3) ◽  
pp. 433-440
Author(s):  
H. G. Davis ◽  
L. M. McDonough ◽  
P. S. Chapman ◽  
C. L. Smithhisler ◽  
D. D. Bowers ◽  
...  

2013 ◽  
Vol 68 (9-10) ◽  
pp. 416-427 ◽  
Author(s):  
Peng-Fei Lu ◽  
Hai-Li Qiao ◽  
You-Qing Luo

The legume pod borer, Maruca vitrata (Lepidoptera: Crambidae; syn. M. testulalis), is a serious pantropical insect pest of grain legumes. Comparative studies of M. vitrata female sex pheromone components in two different geographic populations in China, Wuhan and Huazhou, confi rmed that (E,E)-10,12-hexadecadienal (E10,E12 - 16:Ald) and (E)-10-hexadecenal (E10 - 16:Ald) were present in variable ratios in all pheromone gland extracts of both populations. (E,E)-10,12-hexadecadienol (E10,E12 - 16:OH) was always detected in minor amounts using polar DB-WAX columns, but was never detected using medium-polar DB-17 columns for the two populations. E10 - 16:OH was not found in any of the M. vitrata sex pheromone gland extracts. The average ratios of E10 - 16:Ald, E10,E12 - 16:Ald, and E10,E12 - 16:OH in the pheromone gland extracts of populations from Wuhan and Huazhou were 79.5:100:12.1 and 10.3:100:0.7, respectively. Electrophysiological testing suggested that E10,E12 - 16:Ald elicited the highest male electroantennography (EAG) response, followed by E10,E12 - 16:OH and E10 - 16:Ald. Field-trapping tests with single synthetic sex pheromone lures showed that E10,E12 - 16:OH alone could not attract males, whereas E10,E12 - 16:Ald or E10 - 16:Ald alone attracted few males. Wuhan and Huazhou males were most attracted by lures containing E10 - 16:Ald + E10,E12 - 16:Ald + E10,E12 - 16:OH in doses of (80 + 100 + 10) μg and (10 + 100 + 10) μg, respectively, per vial. Males could discriminate between the blends that were most attractive to their own geographic population and those that were most attractive to the reference population. Our fi ndings suggest that geographic variation exists in the sex pheromone system of M. vitrata in China. The results are discussed with regard to the mechanisms underlying the sex pheromone variation


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