Development of Diagnostic Monitoring Techniques for Larval Pyrethroid Resistance in Heliothis spp. (Lepid Resistance Monitoring in H. virescensoptera: Noctuidae) in Cotton

1989 ◽  
Vol 82 (6) ◽  
pp. 1502-1507 ◽  
Author(s):  
B. F. McCutchen ◽  
F. W. Plapp ◽  
S. J. Nemec ◽  
C. Campanhola
Keyword(s):  
Pyrethroid Resistance ◽  
Resistance Monitoring ◽  
Monitoring Techniques ◽  
Heliothis Spp

Monitoring Pyrethroid Resistance in Bollworm (Lepidoptera: Noctuidae) Moths in Missouri, 1988 to 1994

1998 ◽  
Vol 33 (3) ◽  
pp. 300-312 ◽  
Author(s):  
Clyde E. Sorenson ◽  
Alan Schreiber ◽  
Howard G. Townsend ◽  
Samir F. Abd-Elghafar ◽  
Mahlon L. Fairchild ◽  
...  
Keyword(s):  
Long Range ◽  
Helicoverpa Zea ◽  
Pyrethroid Resistance ◽  
Pheromone Traps ◽  
Pyrethroid Insecticides ◽  
Resistance Monitoring ◽  
Number Of Individuals ◽  
Lepidoptera Noctuidae

From 1988 to 1994, adult vial bioassays were conducted on bollworms, Helicoverpa zea (Boddie), collected from pheromone traps in Missouri to determine their susceptibility to pyrethroids. Although most moths were susceptible to cypermethrin, many assays contained individuals that survived concentrations of 5 and 10 μg per vial. The number of individuals that survived these concentrations increased each of the first 3 yrs, and then fluctuated from year to year. In some cases, moths with increased tolerance to cypermethrin occurred in locations where little or no pyrethroid insecticides were used for bollworm control. A likely explanation for tolerant bollworms in Missouri is immigration from more southerly locations, and evidence for long range dispersal of these insects is presented. Implications for regional resistance monitoring also are discussed.

scholarly journals Establishment of Quantitative Sequencing and Filter Contact Vial Bioassay for Monitoring Pyrethroid Resistance in the Common Bed Bug, Cimex lectularius

2010 ◽  
Vol 47 (4) ◽  
pp. 592-599 ◽  
Author(s):  
Keon Mook Seong ◽  
Da-Young Lee ◽  
Kyong Sup Yoon ◽  
Deok Ho Kwon ◽  
Heung Chul Kim ◽  
...  
Keyword(s):  
Pyrethroid Resistance ◽  
Allele Frequencies ◽  
Resistance Allele ◽  
Monitoring Tool ◽  
Bed Bugs ◽  
Mutation Site ◽  
Signal Ratio ◽  
Α Subunit ◽  
Bed Bug ◽  
Resistance Monitoring

Abstract Two point mutations (V419L and L925I) in the voltage-sensitive sodium channel α-subunit gene have been identified in deltamethrin-resistant bed bugs. A quantitative sequencing (QS) protocol was developed to establish a population-based genotyping method as a molecular resistance-monitoring tool based on the frequency of the two mutations. The nucleotide signal ratio at each mutation site was generated from sequencing chromatograms and plotted against the corresponding resistance allele frequency. Frequency prediction equations were generated from the plots by linear regression, and the signal ratios were shown to highly correlate with resistance allele frequencies (r2 > 0.9928). As determined by QS, neither mutation was found in a bed bug population collected in 1993. Populations collected in recent years (2007–2009), however, exhibited completely or nearly saturating L925I mutation frequencies and highly variable frequencies of the V419L mutation. In addition to QS, the filter contact vial bioassay (FCVB) method was established and used to determine the baseline susceptibility and resistance of bed bugs to deltamethrin and λ-cyhalothrin. A pyrethroid-resistant strain showed >9,375- and 6,990-fold resistance to deltamethrin and λ-cyhalothrin, respectively. Resistance allele frequencies in different bed bug populations predicted by QS correlated well with the FCVB results, confirming the roles of the two mutations in pyrethroid resistance. Taken together, employment of QS in conjunction with FCVB should greatly facilitate the detection and monitoring of pyrethroid-resistant bed bugs in the field. The advantages of FCVB as an on-site resistance-monitoring tool are discussed.

Subsea Insulation Resistance Monitoring

2016 ◽  
Author(s):  
Martijn Wisselink ◽  
Kittipong Somboonpakdeekun ◽  
Suphawat Kiertkul
Keyword(s):  
Insulation Resistance ◽  
Resistance Monitoring
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