Selection of the Western Predatory Mite, Metaseiulus occidentalis (Acari: Phytoseiidae), for Resistance to Abamectin

1989 ◽  
Vol 82 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Marjorie A. Hoy ◽  
Yu-Ling Ouyang
Keyword(s):  
Predatory Mite ◽  
Metaseiulus Occidentalis ◽  
Selection Of

Mitochondrial genome analysis of the predatory mite Phytoseiulus persimilis and a revisit of the Metaseiulus occidentalis mitochondrial genome

Genome ◽  
2010 ◽  
Vol 53 (4) ◽  
pp. 285-301 ◽  
Author(s):  
Wannes Dermauw ◽  
Bartel Vanholme ◽  
Luc Tirry ◽  
Thomas Van Leeuwen
Keyword(s):  
Mitochondrial Genome ◽  
Phytoseiulus Persimilis ◽  
Predatory Mite ◽  
12S Rrna ◽  
Closely Related Species ◽  
Protein Coding ◽  
Hybridization Data ◽  
Metaseiulus Occidentalis ◽  
Standard Set ◽  
Mt Genome

In this study we sequenced and analysed the complete mitochondrial (mt) genome of the Chilean predatory mite Phytoseiulus persimilis Athias-Henriot (Chelicerata: Acari: Mesostigmata: Phytoseiidae: Amblyseiinae). The 16 199 bp genome (79.8% AT) contains the standard set of 13 protein-coding and 24 RNA genes. Compared with the ancestral arthropod mtDNA pattern, the gene order is extremely reshuffled (35 genes changed position) and represents a novel arrangement within the arthropods. This is probably related to the presence of several large noncoding regions in the genome. In contrast with the mt genome of the closely related species Metaseiulus occidentalis (Phytoseiidae: Typhlodrominae) — which was reported to be unusually large (24 961 bp), to lack nad6 and nad3 protein-coding genes, and to contain 22 tRNAs without T-arms — the genome of P. persimilis has all the features of a standard metazoan mt genome. Consequently, we performed additional experiments on the M. occidentalis mt genome. Our preliminary restriction digests and Southern hybridization data revealed that this genome is smaller than previously reported. In addition, we cloned nad3 in M. occidentalis and positioned this gene between nad4L and 12S-rRNA on the mt genome. Finally, we report that at least 15 of the 22 tRNAs in the M. occidentalis mt genome can be folded into canonical cloverleaf structures similar to their counterparts in P. persimilis.

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