Genetic Sexing Systems in Anopheles arabiensis Patton (Diptera: Culicidae)

1985 ◽  
Vol 78 (4) ◽  
pp. 848-851 ◽  
Author(s):  
J. D. Lines ◽  
C. F. Curtis
Keyword(s):  
Anopheles Arabiensis ◽  
Genetic Sexing ◽  
Sexing Systems

Development of a genetic sexing strain of Anopheles arabiensis for KwaZulu-Natal, South Africa

2017 ◽  
Vol 32 (1) ◽  
pp. 61-69 ◽  
Author(s):  
L. C. DANDALO ◽  
G. MUNHENGA ◽  
M. L. KAISER ◽  
L. L. KOEKEMOER
Keyword(s):  
South Africa ◽  
Anopheles Arabiensis ◽  
Genetic Sexing ◽  
Kwazulu Natal ◽  
Genetic Sexing Strain

scholarly journals Male crossing over and genetic sexing systems in the Australian sheep blowfly Lucilia cuprina

Heredity ◽  
1991 ◽  
Vol 67 (3) ◽  
pp. 365-371 ◽  
Author(s):  
Geoffrey G Foster ◽  
Gaye L Weller ◽  
Geoffrey M Clarke
Keyword(s):  
Lucilia Cuprina ◽  
Crossing Over ◽  
Genetic Sexing ◽  
Australian Sheep ◽  
Sexing Systems

scholarly journals Exploiting a Y chromosome-linked Cas9 for sex selection and gene drive

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Stephanie Gamez ◽  
Duverney Chaverra-Rodriguez ◽  
Anna Buchman ◽  
Nikolay P. Kandul ◽  
Stelia C. Mendez-Sanchez ◽  
...  
Keyword(s):  
Genetic Control ◽  
Y Chromosome ◽  
Population Control ◽  
Sex Selection ◽  
Specific Gene ◽  
Gene Drive ◽  
Genetic Sexing ◽  
Animal Populations ◽  
Sexing Systems ◽  
Heterogametic Sex

AbstractCRISPR-based genetic engineering tools aimed to bias sex ratios, or drive effector genes into animal populations, often integrate the transgenes into autosomal chromosomes. However, in species with heterogametic sex chromsomes (e.g. XY, ZW), sex linkage of endonucleases could be beneficial to drive the expression in a sex-specific manner to produce genetic sexing systems, sex ratio distorters, or even sex-specific gene drives, for example. To explore this possibility, here we develop a transgenic line of Drosophila melanogaster expressing Cas9 from the Y chromosome. We functionally characterize the utility of this strain for both sex selection and gene drive finding it to be quite effective. To explore its utility for population control, we built mathematical models illustrating its dynamics as compared to other state-of-the-art systems designed for both population modification and suppression. Taken together, our results contribute to the development of current CRISPR genetic control tools and demonstrate the utility of using sex-linked Cas9 strains for genetic control of animals.

scholarly journals Additional evidence on the efficacy of different Akirin vaccines assessed on Anopheles arabiensis (Diptera: Culicidae)

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Blaženka D. Letinić ◽  
Marinela Contreras ◽  
Yael Dahan-Moss ◽  
Ingrid Linnekugel ◽  
José de la Fuente ◽  
...  
Keyword(s):  
Vector Control ◽  
Aedes Albopictus ◽  
Malaria Vector ◽  
Vaccine Efficacy ◽  
Anopheles Arabiensis ◽  
Vaccine Development ◽  
Target Antigen ◽  
Control Methods ◽  
Protective Response ◽  
Current Vector

Abstract Background Anopheles arabiensis is an opportunistic malaria vector that rests and feeds outdoors, circumventing current indoor vector control methods. Furthermore, this vector will readily feed on both animals and humans. Targeting this vector while feeding on animals can provide an additional intervention for the current vector control activities. Previous results have displayed the efficacy of using Subolesin/Akirin ortholog vaccines for the control of multiple ectoparasite infestations. This made Akirin a potential antigen for vaccine development against An. arabiensis. Methods The efficacy of three antigens, namely recombinant Akirin from An. arabiensis, recombinant Akirin from Aedes albopictus, and recombinant Q38 (Akirin/Subolesin chimera) were evaluated as novel interventions for An. arabiensis vector control. Immunisation trials were conducted based on the concept that mosquitoes feeding on vaccinated balb/c mice would ingest antibodies specific to the target antigen. The antibodies would interact with the target antigen in the arthropod vector, subsequently disrupting its function. Results All three antigens successfully reduced An. arabiensis survival and reproductive capacities, with a vaccine efficacy of 68–73%. Conclusions These results were the first to show that hosts vaccinated with recombinant Akirin vaccines could develop a protective response against this outdoor malaria transmission vector, thus providing a step towards the development of a novel intervention for An. arabiensis vector control. Graphic Abstract

scholarly journals Genetic analysis of medfly populations in an area of sterile insect technique applications

2021 ◽  
Author(s):  
Rubén Sancho ◽  
Ana Guillem-Amat ◽  
Elena López-Errasquín ◽  
Lucas Sánchez ◽  
Félix Ortego ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Sterile Insect Technique ◽  
Ceratitis Capitata ◽  
Natural Populations ◽  
Low Frequency ◽  
Fruit Fly ◽  
Wild Populations ◽  
Genetic Sexing ◽  
Environmental Implications ◽  
Genetic Sexing Strains

AbstractThe sterile insect technique (SIT) is widely used in integrated pest management programs for the control of the Mediterranean fruit fly (medfly), Ceratitis capitata. The genetic interactions between the released individuals from the genetic sexing strains (GSS), used for SIT applications worldwide, and wild individuals have not been studied. Under the hypothesis that a number of Vienna GSS individuals released to the field might not be completely sterile and may produce viable offspring, we have analyzed medfly Spanish field populations to evaluate the presence of Vienna strain genetic markers. To this goal, we have used contrasted nuclear and mitochondrial genetic markers, and two novel sets of nuclear polymorphisms with the potential to be markers to discriminate between Vienna and wild individuals. Nuclear Vienna markers located on the 5th chromosome of Vienna males have been found in 2.2% (19 from 875) of the Spanish wild medfly females captured at the area where SIT is applied. In addition, a female-inherited mitochondrial Vienna marker has been found in two from the 19 females showing nuclear Vienna markers. The detection of several of these markers in single individuals represents evidence of the introgression of Vienna strain into natural populations. However, alternative explanations as their presence at low frequency in wild populations in the studied areas cannot be fully discarded. The undesired release of non-fully sterile irradiated GSS individuals into the field and their interactions with wild flies, and the potential environmental implications should be taken into account in the application of the SIT.

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