Peripheral tissue levels and molecular species compositions of N-acyl-phosphatidylethanolamine and its metabolites in mice lacking N-acyl-phosphatidylethanolamine-specific phospholipase D

2017 ◽  
Vol 162 (6) ◽  
pp. 449-458 ◽  
Author(s):  
Manami Inoue ◽  
Kazuhito Tsuboi ◽  
Yoko Okamoto ◽  
Mayumi Hidaka ◽  
Toru Uyama ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Molecular Species ◽  
Peripheral Tissue ◽  
Tissue Levels

scholarly journals Alterations of the endocannabinoid system and circulating and peripheral tissue levels of endocannabinoids in sarcopenic rats

2021 ◽  
Author(s):  
Olivier Le Bacquer ◽  
Jérôme Salles ◽  
Fabiana Piscitelli ◽  
Phelipe Sanchez ◽  
Vincent Martin ◽  
...  
Keyword(s):  
Endocannabinoid System ◽  
Peripheral Tissue ◽  
Tissue Levels

scholarly journals Molecular species of phospholipids in a murine stem-cell line responsive to erythropoietin

1996 ◽  
Vol 314 (3) ◽  
pp. 861-867 ◽  
Author(s):  
Barbara S. BECKMAN ◽  
Conrad MALLIA ◽  
Sanda CLEJAN
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Phosphatidic Acid ◽  
Phospholipase D ◽  
Molecular Species ◽  
Direct Action ◽  
Stem Cell Line ◽  
Signalling Molecules ◽  
Lipid Signalling ◽  
Over Time

The generation of the lipid signalling molecules, diacylglycerol (DAG) and phosphatidic acid (PA), has been implicated in the transduction events essential for proliferation of murine B6SUt.EP stem cells responsive to erythropoietin (EPO). Some of the responses were rapid and transient while others were slower and sustained. In an attempt to better understand the biphasic nature of DAG and PA appearance in response to EPO, an analysis of the molecular species of DAG, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), and PA in control and EPO-treated B6SUt.EP cells was made by HPLC and TLC. Fifteen to eighteen species were identified, which were increased non-uniformly by 0.2 unit/ml EPO. Greater increases (×6) were observed in 16:0,20:4 and 18:0,20:4 DAGs than in other species. The molecular species profiles of the stimulated DAGs were compared with the profiles of molecular species contained in the phospholipids. Comparison of the increase in DAG species caused by EPO with the molecular species present in PC and PI showed both PI and PC as the source of the fast DAG accumulation and only PC as the source of the slow DAG accumulation. PE was involved in both phases. We found a consistent formation of ethanolamine over time, in larger amounts than choline, providing strong evidence that, in addition to PC, PE is a major substrate. In addition, changes in molecular species of PA in response to EPO suggest that PI cannot account for the mass of PA formed during the first 30 s incubation with EPO, nor for PA formed during 30 min with EPO. It is concluded that the majority of PA was formed by a direct action of phospholipase D on PC.

Dietary fatty acids augment tissue levels of n-acylethanolamines in n-acylphosphatidylethanolamine phospholipase D (NAPE-PLD) knockout mice

2018 ◽  
Vol 62 ◽  
pp. 134-142 ◽  
Author(s):  
Lin Lin ◽  
Adam H Metherel ◽  
Alex P Kitson ◽  
Shoug M Alashmali ◽  
Kathryn E Hopperton ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Phospholipase D ◽  
Knockout Mice ◽  
Dietary Fatty Acids ◽  
Tissue Levels

Trace Element Contents in Thyroid Cancer Investigated by Instrumental Neutron Activation Analysis

2018 ◽  
Vol 1 (1) ◽  
pp. 1-13
Keyword(s):  
Thyroid Cancer ◽  
Neutron Activation Analysis ◽  
Instrumental Neutron Activation Analysis ◽  
Activation Analysis ◽  
Neutron Activation ◽  
Trace Element ◽  
Thyroid Tissue ◽  
Tissue Levels ◽  
Trace Element Contents ◽  
Element Contents

Background: Thyroid cancer is an internationally important health problem. The aim of this exploratory study was to evaluate whether significant changes in the thyroid tissue levels of Ag, Co, Cr, Fe, Hg, Rb, Sb, Sc, Se, and Zn exist in the malignantly transformed thyroid. Methods: Thyroid tissue levels of ten trace elements were prospectively evaluated in 41 patients with thyroid malignant tumors and 105 healthy inhabitants. Measurements were performed using non-destructive instrumental neutron activation analysis with high resolution spectrometry of long-lived radionuclides. Tissue samples were divided into two portions. One was used for morphological study while the other was intended for trace element analysis. Results: It was found that contents of Ag, Co, Cr, Hg, and Rb were significantly higher (approximately 12.8, 1.4, 1.6, 19.6, and 1.7 times, respectively) in cancerous tissues than in normal tissues. Conclusions: There are considerable changes in trace element contents in the malignantly transformed tissue of thyroid.

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