DNA-binding activity of rat DNA topoisomerase II α C-terminal domain contributes to efficient DNA catenationin vitro

2015 ◽  
pp. mvv110 ◽  
Author(s):  
Shinji Kawano ◽  
Yuri Kato ◽  
Natsumi Okada ◽  
Kuniaki Sano ◽  
Ken Tsutsui ◽  
...  
Keyword(s):  
Dna Binding ◽  
Topoisomerase Ii ◽  
Binding Activity ◽  
Dna Topoisomerase Ii ◽  
Dna Topoisomerase ◽  
Terminal Domain ◽  
Dna Binding Activity ◽  
Topoisomerase Ii Α

The C-terminal domain of Saccharomyces cerevisiae DNA topoisomerase II

1994 ◽  
Vol 14 (5) ◽  
pp. 3197-3207
Author(s):  
P R Caron ◽  
P Watt ◽  
J C Wang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nuclear Localization ◽  
Topoisomerase Ii ◽  
Dna Topoisomerase Ii ◽  
Dna Topoisomerase ◽  
Mutant Proteins ◽  
Terminal Domain ◽  
Catalytically Active

A set of carboxy-terminal deletion mutants of Saccharomyces cerevisiae DNA topoisomerase II were constructed for studying the functions of the carboxyl domain in vitro and in vivo. The wild-type yeast enzyme is a homodimer with 1,429 amino acid residues in each of the two polypeptides; truncation of the C terminus to Ile-1220 has little effect on the function of the enzyme in vitro or in vivo, whereas truncations extending beyond Gln-1138 yield completely inactive proteins. Several mutant enzymes with C termini in between these two residues were found to be catalytically active but unable to complement a top2-4 temperature-sensitive mutation. Immunomicroscopy results suggest that the removal of a nuclear localization signal in the C-terminal domain is likely to contribute to the physiological dysfunction of these proteins; the ability of these mutant proteins to relax supercoiled DNA in vivo shows, however, that at least some of the mutant proteins are present in the nuclei in a catalytically active form. In contrast to the ability of the catalytically active mutant proteins to relax supercoiled intracellular DNA, all mutants that do not complement the temperature-dependent lethality and high frequency of chromosomal nondisjunction of top2-4 were found to lack decatenation activity in vivo. The plausible roles of the DNA topoisomerase II C-terminal domain, in addition to providing a signal for nuclear localization, are discussed in the light of these results.

scholarly journals DNA-binding Activity of the ERp57 C-terminal Domain Is Related to a Redox-dependent Conformational Change

2007 ◽  
Vol 282 (14) ◽  
pp. 10299-10310 ◽  
Author(s):  
Caterina Grillo ◽  
Chiara D'Ambrosio ◽  
Valerio Consalvi ◽  
Roberta Chiaraluce ◽  
Andrea Scaloni ◽  
...  
Keyword(s):  
Dna Binding ◽  
Conformational Change ◽  
Binding Activity ◽  
Terminal Domain ◽  
Dna Binding Activity

Interaction of Human DNA Topoisomerase II α with DNA:  Quantification by Surface Plasmon Resonance†

Biochemistry ◽  
2002 ◽  
Vol 41 (45) ◽  
pp. 13395-13402 ◽  
Author(s):  
Axelle Renodon-Cornière ◽  
Lars H. Jensen ◽  
John L. Nitiss ◽  
Peter B. Jensen ◽  
Maxwell Sehested
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Topoisomerase Ii ◽  
Dna Quantification ◽  
Dna Topoisomerase Ii ◽  
Dna Topoisomerase ◽  
Human Dna ◽  
Topoisomerase Ii Α

Atypical multidrug resistance may be associated with catalytically active mutants of human DNA topoisomerase II α

Gene ◽  
2001 ◽  
Vol 272 (1-2) ◽  
pp. 141-148 ◽  
Author(s):  
Yoshito Okada ◽  
Aki Tosaka ◽  
Yuji Nimura ◽  
Akihiko Kikuchi ◽  
Shonen Yoshida ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Topoisomerase Ii ◽  
Dna Topoisomerase Ii ◽  
Dna Topoisomerase ◽  
Human Dna ◽  
Catalytically Active ◽  
Topoisomerase Ii Α

scholarly journals Mitotic Phosphorylation of DNA Topoisomerase II α by Protein Kinase CK2 Creates the MPM-2 Phosphoepitope on Ser-1469

2000 ◽  
Vol 275 (44) ◽  
pp. 34710-34718 ◽  
Author(s):  
Alexandre E. Escargueil ◽  
Sergei Y. Plisov ◽  
Odile Filhol ◽  
Claude Cochet ◽  
Annette K. Larsen
Keyword(s):  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Topoisomerase Ii ◽  
Dna Topoisomerase Ii ◽  
Dna Topoisomerase ◽  
Topoisomerase Ii Α ◽  
Mitotic Phosphorylation ◽  
Kinase Ck2

scholarly journals The Impact of the Human DNA Topoisomerase II C-Terminal Domain on Activity

PLoS ONE ◽  
2008 ◽  
Vol 3 (3) ◽  
pp. e1754 ◽  
Author(s):  
Emma L. Meczes ◽  
Kathryn L. Gilroy ◽  
Katherine L. West ◽  
Caroline A. Austin
Keyword(s):  
Topoisomerase Ii ◽  
Dna Topoisomerase Ii ◽  
Dna Topoisomerase ◽  
Terminal Domain ◽  
Human Dna ◽  
The Impact
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