Efficient expression and purification of recombinant human m-calpain using an Escherichia coli expression system at low temperature

2012 ◽  
Vol 151 (4) ◽  
pp. 417-422 ◽  
Author(s):  
S. Hata ◽  
M. Ueno ◽  
F. Kitamura ◽  
H. Sorimachi
Keyword(s):  
Escherichia Coli ◽  
Low Temperature ◽  
Expression System ◽  
Expression And Purification ◽  
Efficient Expression ◽  
Escherichia Coli Expression

Escherichia coli expression and purification of recombinant dog albumin, a cross-reactive animal allergen

2000 ◽  
Vol 105 (2) ◽  
pp. 279-285 ◽  
Author(s):  
Budhi Pandjaitan ◽  
Ines Swoboda ◽  
Franziska Brandejsky-Pichler ◽  
Helmut Rumpold ◽  
Rudolf Valenta ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Expression And Purification ◽  
Escherichia Coli Expression ◽  
Animal Allergen

scholarly journals Construction of an antibody Fv-Epo receptor chimera extracellular domainusing Escherichia coli expression system

2000 ◽  
Vol 40 (supplement) ◽  
pp. S186
Author(s):  
Takeshi Kitamura ◽  
Kouhei Tsumoto ◽  
Masahiro Kawahara ◽  
Hiroshi Ueda ◽  
Teruyuki Nagamune ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Expression System ◽  
Epo Receptor ◽  
Escherichia Coli Expression

Expression of adenylate kinase fused mouse ubiquitin active enzyme in Escherichia coli and its application in ubiquitination

2021 ◽  
Author(s):  
Xiaoliang Liu ◽  
Ling Hu ◽  
Yuan Zhang ◽  
Hongtao Li
Keyword(s):  
Escherichia Coli ◽  
Catalytic Activity ◽  
Chemical Synthesis ◽  
Adenylate Kinase ◽  
Energy Supply ◽  
Expression System ◽  
Synthesis Process ◽  
Cellular Functions ◽  
Escherichia Coli Expression

Abstract Ubiquitination, is involved in the regulation of numerous cellular functions. Researches in the ubiquitin realm rely heavily on ubiquitination assays in vitro and require large amounts of ubiquitin-activating enzyme (UBA1) and keep ATP supplies. But UBA1 is hard to be obtained with large quantities using reported methods. We fused Escherichia coli adenylate kinase (adk) and mouse UBA1 obtained fusion protein adk-mUBA1. The expression level of adk-mUBA1 increased about 8-fold than that of mUBA1 in Escherichia coli expression system, and adk-mUBA1 was easily purified to 90% purity via two purification steps. The purified adk-mUBA1 protein was functional for ubiquitination and could use ATP in addition to ADP as energy supply and had a higher catalytic activity than mUBA1 in cell lysis. Adk-mUBA1 can be applied to preparing ubiquitin modified substrates and kinds of ubiquitin chains in chemical synthesis process and is preferable application than mUBA1 in vitro ubiquitination.

Cotranslational Incorporation of a Structurally Diverse Series of Proline Analogues in an Escherichia coli Expression System

ChemBioChem ◽  
2004 ◽  
Vol 5 (7) ◽  
pp. 928-936 ◽  
Author(s):  
Wookhyun Kim ◽  
Anna George ◽  
Melissa Evans ◽  
Vincent P. Conticello
Keyword(s):  
Escherichia Coli ◽  
Expression System ◽  
Escherichia Coli Expression

Effect of rhBMP-2 produced by Escherichia coli expression system on bone formation in rat calvarial defects

2009 ◽  
Vol 39 (1) ◽  
pp. 77
Author(s):  
Suk-Hoon Kwon ◽  
Hyun-Chang Lim ◽  
Kyung-Hee Choi ◽  
Min-Soo Kim ◽  
Ji-Hyun Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bone Formation ◽  
Expression System ◽  
Calvarial Defects ◽  
Escherichia Coli Expression
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