Characterization of Type I Collagen Fibril Formation Using Thioflavin T Fluorescent Dye

2009 ◽  
Vol 145 (5) ◽  
pp. 677-684 ◽  
Author(s):  
K. Morimoto ◽  
K. Kawabata ◽  
S. Kunii ◽  
K. Hamano ◽  
T. Saito ◽  
...  
Keyword(s):  
Collagen Fibril ◽  
Type I Collagen ◽  
Fibril Formation ◽  
Fluorescent Dye ◽  
Thioflavin T ◽  
Type I

scholarly journals The Mammalian Chitinase-like Lectin, YKL-40, Binds Specifically to Type I Collagen and Modulates the Rate of Type I Collagen Fibril Formation

2006 ◽  
Vol 281 (30) ◽  
pp. 21082-21095 ◽  
Author(s):  
Heather F. Bigg ◽  
Robin Wait ◽  
Andrew D. Rowan ◽  
Tim E. Cawston
Keyword(s):  
Collagen Fibril ◽  
Type I Collagen ◽  
Fibril Formation ◽  
Type I

scholarly journals Osteopontin regulates type I collagen fibril formation in bone tissue

2020 ◽  
Author(s):  
Baptiste Depalle ◽  
Catriona M. McGilvery ◽  
Sabah Nobakhti ◽  
Nouf Aldegaither ◽  
Sandra J. Shefelbine ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Collagen Fibril ◽  
Type I Collagen ◽  
Fibril Formation ◽  
Type I

Effect of UV irradiation on type I collagen fibril formation in neutral collagen solutions

2001 ◽  
Vol 17 (3) ◽  
pp. 114-120 ◽  
Author(s):  
Julian M. Menter ◽  
Abrienne M. Patta ◽  
Robert M. Sayre ◽  
John Dowdy ◽  
Isaac Willis
Keyword(s):  
Uv Irradiation ◽  
Collagen Fibril ◽  
Type I Collagen ◽  
Fibril Formation ◽  
Type I

scholarly journals Structure–function studies on Hsp47: pH-dependent inhibition of collagen fibril formation in vitro

2000 ◽  
Vol 349 (3) ◽  
pp. 877-883 ◽  
Author(s):  
Christy A. THOMSON ◽  
Vettai S. ANANTHANARAYANAN
Keyword(s):  
Endoplasmic Reticulum ◽  
Collagen Fibril ◽  
Type I Collagen ◽  
Fibril Formation ◽  
Molar Ratio ◽  
Functional Assay ◽  
Type I ◽  
Ph Dependent ◽  
Ph Range

Hsp47, a 47kDa heat shock protein whose expression level parallels that of collagen, has been regarded as a collagen-specific molecular chaperone. Studies from other laboratories have established the association of Hsp47 with the nascent as well as the triple-helical procollagen molecule in the endoplasmic reticulum and its dissociation from procollagen in the Golgi. One of several roles suggested for Hsp47 in collagen biosynthesis is the prevention of aggregation of procollagen in the endoplasmic reticulum. However, no experimental evidence has been available to verify this suggestion. In the present study we have followed the aggregation of mature triple-helical collagen molecules into fibrils by using turbidimetric measurements in the absence and presence of Hsp47. In the pH range 6–7, fibril formation of type I collagen, as monitored by turbidimetry, proceeds with a lag of approx. 10min and levels off by approx. 60min. The addition of Hsp47 at pH 7 effectively inhibits fibril formation at and above a 1:1 molar ratio of Hsp47 to triple-helical collagen. This inhibition is markedly pH-dependent, being significantly diminished at pH 6. CD and fluorescence spectral data of Hsp47 in the pH range 4.2–7.4 reveal a significant alteration in its structure at pH values below 6.2, with a decrease in α-helix and an increase in β-structure. This conformational change is likely to be the basis of the decreased binding of Hsp47 to collagen in vitro at pH 6.3 as well as its inability to inhibit collagen fibril formation at this pH. Our results also provide a functional assay for Hsp47 that can be used in studies on collagen and Hsp47 interactions.

scholarly journals Structural Property of Decorin from Small Intestine and its Effect of Type I Collagen Fibril Formation

1998 ◽  
Vol 69 (8) ◽  
pp. 779-791
Author(s):  
Yoshihiro NOMURA ◽  
Takahiro MIZUTANI ◽  
Yasuhiro ISHII ◽  
Kunio SHIRAI
Keyword(s):  
Small Intestine ◽  
Structural Property ◽  
Collagen Fibril ◽  
Type I Collagen ◽  
Fibril Formation ◽  
Type I

Detection of type I collagen fibrils formation and dissociation by a fluorescence method based on thioflavin T

2016 ◽  
Vol 92 ◽  
pp. 1175-1182 ◽  
Author(s):  
Meilian Zou ◽  
Huan Yang ◽  
Haibo Wang ◽  
Haiyin Wang ◽  
Juntao Zhang ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Collagen Fibrils ◽  
Fluorescence Method ◽  
Thioflavin T ◽  
Type I
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