Effect of Forced Expression of Basic Fibroblast Growth Factor in Human Bone Marrow-Derived Mesenchymal Stromal Cells

2007 ◽  
Vol 142 (6) ◽  
pp. 741-748 ◽  
Author(s):  
M. J. Go ◽  
C. Takenaka ◽  
H. Ohgushi
Keyword(s):  
Bone Marrow ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Fibroblast Growth

Age-dependent decrease in the chondrogenic potential of human bone marrow mesenchymal stromal cells expanded with fibroblast growth factor-2

Cytotherapy ◽  
2013 ◽  
Vol 15 (9) ◽  
pp. 1062-1072 ◽  
Author(s):  
Masami Kanawa ◽  
Akira Igarashi ◽  
Veronica Sainik Ronald ◽  
Yukihito Higashi ◽  
Hidemi Kurihara ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Human Bone ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Chondrogenic Potential ◽  
Age Dependent

Long-Term Culture of Human Bone Marrow Stromal Cells in the Presence of Basic Fibroblast Growth Factor

1990 ◽  
Vol 3 (3) ◽  
pp. 231-236 ◽  
Author(s):  
Lisa I. Oliver ◽  
Daniel B. Rifkin ◽  
Janice Gabrilove ◽  
Melanie-Jane Hannocks ◽  
E. Lynette Wilson
Keyword(s):  
Bone Marrow ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Fibroblast Growth

scholarly journals Fibroblast Growth Factor 2 Regulates High Mobility Group A2 Expression in Human Bone Marrow-Derived Mesenchymal Stem Cells

2016 ◽  
Vol 117 (9) ◽  
pp. 2128-2137 ◽  
Author(s):  
Stefanos Kalomoiris ◽  
Andrew C. Cicchetto ◽  
Kinga Lakatos ◽  
Jan A. Nolta ◽  
Fernando A. Fierro
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
High Mobility ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth

scholarly journals Remodeling of Human Osteochondral Defects via rAAV-Mediated Co-Overexpression of TGF-β and IGF-I from Implanted Human Bone Marrow-Derived Mesenchymal Stromal Cells

2019 ◽  
Vol 8 (9) ◽  
pp. 1326
Author(s):  
Stephanie Morscheid ◽  
Jagadeesh Kumar Venkatesan ◽  
Ana Rey-Rico ◽  
Gertrud Schmitt ◽  
Magali Cucchiarini
Keyword(s):  
Bone Marrow ◽  
Growth Factor ◽  
Gene Transfer ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Human Model ◽  
Cartilage Lesions ◽  
Igf I

The application of chondrogenic gene sequences to human bone marrow-derived mesenchymal stromal cells (hMSCs) is an attractive strategy to activate the reparative activities of these cells as a means to enhance the processes of cartilage repair using indirect cell transplantation procedures that may improve the repopulation of cartilage lesions. In the present study, we examined the feasibility of co-delivering the highly competent transforming growth factor beta (TGF-β) with the insulin-like growth factor I (IGF-I) in hMSCs via recombinant adeno-associated virus (rAAV) vector-mediated gene transfer prior to implantation in a human model of osteochondral defect (OCD) ex vivo that provides a microenvironment similar to that of focal cartilage lesions. The successful co-overexpression of rAAV TGF-β/IGF-I in implanted hMSCs promoted the durable remodeling of tissue injury in human OCDs over a prolonged period of time (21 days) relative to individual gene transfer and the control (reporter lacZ gene) treatment, with enhanced levels of cell proliferation and matrix deposition (proteoglycans, type-II collagen) both in the lesions and at a distance, while hypertrophic, osteogenic, and catabolic processes could be advantageously delayed. These findings demonstrate the value of indirect, progenitor cell-based combined rAAV gene therapy to treat human focal cartilage defects in a natural environment as a basis for future clinical applications.

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