Molecular Characterization of Plantain Class I Chitinase Gene and its Expression in Response to Infection by Gloeosporium musarum Cke and Massee and other Abiotic Stimuli

2007 ◽  
Vol 142 (5) ◽  
pp. 561-570 ◽  
Author(s):  
J. Fan ◽  
H. Wang ◽  
D. Feng ◽  
B. Liu ◽  
H. Liu ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Chitinase Gene ◽  
Class I ◽  
Abiotic Stimuli

Identification and Molecular Characterization of a Class I Chitinase Gene (Mhchit1) from Malus hupehensis

2011 ◽  
Vol 30 (3) ◽  
pp. 760-767 ◽  
Author(s):  
Ji-Yu Zhang ◽  
Zhong-Ren Guo ◽  
Shen-Chun Qu ◽  
Zhen Zhang
Keyword(s):  
Molecular Characterization ◽  
Chitinase Gene ◽  
Class I ◽  
Malus Hupehensis

scholarly journals Molecular Characterization of E. Histolytica Strains Based on the Serine Rich Entamoeba Histolytica Protein (Srehp) And Chitinase Genes.

2021 ◽  
Author(s):  
Renay Ngobeni ◽  
Amidou Samie
Keyword(s):  
South Africa ◽  
Molecular Characterization ◽  
Significant Influence ◽  
Genetic Heterogeneity ◽  
Chitinase Gene ◽  
Genetic Profile ◽  
Genetic Characteristics ◽  
Stool Samples ◽  
Chitinase Genes

Abstract BACKGROUND: Even though E. histolytica is recognized as an effective pathogen, what determines the outcome of this infection is still not well understood. The present study was carried out to determine the genetic characteristics of E. histolytica isolates from two different regions in South Africa. METHOD: Diarrheal and non-diarrheal stool samples were collected from patients of all ages from Giyani and Pretoria. Different PCR protocols were used to identify E. histolytica and amplify the serine rich E. histolytica protein (SREHP) and chitinase genes. The profiles obtained were compared among the different samples.RESULTS: Out of 111 stool samples collected, 51 were positive by either PCR or microscopy and 14 samples were positive by both methods. The serine- rich E. histolytica protein was amplified in 26 samples. Out of the 26 samples (19) different SREHP profiles were obtained. SREHP #2 was obtained in 5 different samples, 4 from Pretoria and 1 from Giyani (2 diarrheal and 3 non-diarrheal). The chitinase gene was amplified from 51 samples and 22 different chitinase profiles were obtained. Of all the profiles, profile #4 was found in 6 different isolates, 5 from Giyani and 1 from Pretoria (3 symptomatic and 3 asymptomatic). However, profile # 18 was only found in formed stools from Giyani. CONCLUSIONS. The results obtained in this study have further confirmed the genetic heterogeneity of E. histolytica for the SREHP and chitinase genes which might have a significant influence in the outcome of amebic infection, depending on the genetic profile of the infecting strain.

Molecular characterization of the porcine MHC class I region

2009 ◽  
Vol 25 (5) ◽  
pp. 357-359 ◽  
Author(s):  
U. Gaycken ◽  
M. Shabahang ◽  
J. N. Meyer ◽  
P. Glodek
Keyword(s):  
Molecular Characterization ◽  
Mhc Class I ◽  
Class I ◽  
Class I Region

Cloning and molecular characterization of the Hevea brasiliensis allergen Hev b 11, a class I chitinase

2002 ◽  
Vol 32 (3) ◽  
pp. 455-462 ◽  
Author(s):  
G. O'Riordain ◽  
C. Radauer ◽  
K. Hoffmann-Sommergruber ◽  
F. Adhami ◽  
C. K. Peterbauer ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Hevea Brasiliensis ◽  
Class I
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