Development and Characterization of a Novel Fusion Protein Composed of a Human IgG1 Heavy Chain Constant Region and a Single-Chain Fragment Variable Antibody against Venezuelan Equine Encephalitis Virus

2003 ◽  
Vol 133 (1) ◽  
pp. 59-66 ◽  
Author(s):  
W.-G. Hu
Keyword(s):  
Fusion Protein ◽  
Constant Region ◽  
Venezuelan Equine Encephalitis ◽  
Single Chain ◽  
Chain Fragment ◽  
Equine Encephalitis Virus ◽  
Single Chain Fragment Variable ◽  
Heavy Chain Constant Region ◽  
Human Igg1

Isolation and characterization of anti ROR1 single chain fragment variable antibodies using phage display technique

2017 ◽  
Vol 25 (1-2) ◽  
pp. 57-63 ◽  
Author(s):  
Leili Aghebati-Maleki ◽  
Vahid Younesi ◽  
Farhad Jadidi-Niaragh ◽  
Behzad Baradaran ◽  
Jafar Majidi ◽  
...  
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Chain Fragment ◽  
Phage Display Technique ◽  
Isolation And Characterization ◽  
Single Chain Fragment Variable ◽  
Display Technique ◽  
Single Chain Fragment

Characterization of a CC49-Based Single-Chain Fragment−β-Lactamase Fusion Protein for Antibody-Directed Enzyme Prodrug Therapy (ADEPT)

2006 ◽  
Vol 17 (2) ◽  
pp. 410-418 ◽  
Author(s):  
Ralph F. Alderson ◽  
Brian E. Toki ◽  
Martin Roberge ◽  
Wei Geng ◽  
Joshua Basler ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Single Chain ◽  
Enzyme Prodrug Therapy ◽  
Chain Fragment ◽  
Single Chain Fragment

scholarly journals Generation and characterization of a human single-chain fragment variable (scFv) antibody against cytosine deaminase from Yeast

2008 ◽  
Vol 8 (1) ◽  
pp. 68 ◽  
Author(s):  
Alessandra Mallano ◽  
Silvia Zamboni ◽  
Giulia Carpinelli ◽  
Filippo Santoro ◽  
Michela Flego ◽  
...  
Keyword(s):  
Cytosine Deaminase ◽  
Scfv Antibody ◽  
Single Chain ◽  
Chain Fragment ◽  
Single Chain Fragment Variable ◽  
Single Chain Fragment

scholarly journals In Vitro and In Vivo Characterization of a Novel Antibody-Like Single-Chain TCR Human IgG1 Fusion Protein

2005 ◽  
Vol 174 (7) ◽  
pp. 4381-4388 ◽  
Author(s):  
Luis A. Mosquera ◽  
Kimberlyn F. Card ◽  
Shari A. Price-Schiavi ◽  
Heather J. Belmont ◽  
Bai Liu ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Single Chain ◽  
Human Igg1 ◽  
In Vivo Characterization

scholarly journals Characterization of upFc, a fragment of human immunoglobulin G1 produced by pepsin in urea

1976 ◽  
Vol 157 (3) ◽  
pp. 535-540 ◽  
Author(s):  
D M Parr ◽  
T Hofmann ◽  
G E Connell
Keyword(s):  
Amino Acid ◽  
Light Chains ◽  
Constant Region ◽  
Amino Acid Sequencing ◽  
Chain Fragment ◽  
Heavy Chains ◽  
Myeloma Proteins ◽  
Peptic Digestion ◽  
Human Igg1

The digestion of human IgG1/K myeloma proteins with pepsin in the presence of 8 M-urea produces fragments that differ from those produced by aqueous peptic digestion, and from other characteristic immunoglobulin fragments. Fb′2, the larger urea/pepsin fragment, was previously shown to consist of the constant regions of the light chains, and the CH1 domains and hinge regions of the heavy chains. The smaller fragment, upFc, has now been characterized. After reduction, three peptides were released from fragment upFc. Amino acid sequencing, N- and C-terminal determinations and amino acid compositions have enabled these peptides to be identified as residues Ile-253 to Leu-306, residues Thr-307 to Asp-376 and residues Thr-411 to Gly-446 of the heavy chain. Fragment upFc therefore contains the entire Fc region, beginning at residue Ile-253, except for a 34-residue section from within the CH3-domain disulphide loop. Peptic digestion of IgG1/K proteins in 8M-urea therefore provides a method for isolating from gamma1 heavy chains five homogeneous peptides in good yield, which account for almost the entire constant region. Characterization of fragments Fb′2 and upFc has shown that the action of pepsin in urea is entirely different from that of aqueous pepsin. Two gamma1 heavy chains have been shown to differ in sequence at three positions from the sequence reported for protein Eu.

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