scholarly journals Paper Withdrawn: Quercetin attenuates high glucose-induced injury in human retinal pigment epithelial cell line ARPE-19 by up-regulation of miR-29b

2020 ◽  
Vol 168 (6) ◽  
pp. 677-677
Author(s):  
Xuejiao Wang ◽  
Hui Li ◽  
Hao Wang ◽  
Jingyun Shi
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
High Glucose ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment Epithelial Cell ◽  
Retinal Pigment ◽  
Epithelial Cell Line ◽  
Pigment Epithelial

scholarly journals Reversal of the Caspase-Dependent Apoptotic Cytotoxicity Pathway by Taurine fromLycium barbarum(Goji Berry) in Human Retinal Pigment Epithelial Cells: Potential Benefit in Diabetic Retinopathy

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
M. K. Song ◽  
B. D. Roufogalis ◽  
T. H. W. Huang
Keyword(s):  
Diabetic Retinopathy ◽  
Epithelial Cell ◽  
Cell Line ◽  
High Glucose ◽  
Retinal Pigment Epithelial ◽  
Caspase 3 ◽  
Retinal Pigment ◽  
Epithelial Cell Line ◽  
Cytoprotective Effect ◽  
Pigment Epithelial

Diabetic retinopathy is a preventable microvascular diabetic complication and a leading cause of vision loss. Retinal pigment epithelial cell apoptosis is an early event in diabetic retinopathy. Taurine is reportedly beneficial for diabetic retinopathy and is abundant in the fruit ofLycium barbarum(LB). We have investigated the effect of pure taurine and an extract of LB rich in taurine on a model of diabetic retinopathy, the retinal ARPE-19 cell line exposed to high glucose. We demonstrate for the first time that LB extract and the active ligand, taurine, dose dependently enhance cell viability following high glucose treatment in the ARPE-19 retinal epithelial cell line. This cytoprotective effect was associated with the attenuation of high glucose-induced apoptosis, which was shown by characteristic morphological staining and the dose-dependent decrease in the number of apoptotic cells, determined by flow cytometry. Moreover, we have shown that LB extract and taurine dose dependently downregulate caspase-3 protein expression and the enzymatic activity of caspase-3. We conclude that taurine, a major component of LB, and the LB extract, have a cytoprotective effect against glucose exposure in a human retinal epithelial cell line and may provide useful approaches to delaying diabetic retinopathy progression.

Sign in / Sign up

Export Citation Format

Share Document