scholarly journals The effect of orexin B on steroidogenic acute regulatory protein, P450 side-chain cleavage enzyme, and 3β-hydroxysteroid dehydrogenase gene expression, and progesterone and androstenedione secretion by the porcine uterus during early pregnancy and the estrous cycle1

2018 ◽  
Vol 97 (2) ◽  
pp. 851-864 ◽  
Author(s):  
Katarzyna Kisielewska ◽  
Edyta Rytelewska ◽  
Marlena Gudelska ◽  
Marta Kiezun ◽  
Kamil Dobrzyn ◽  
...  
Keyword(s):  
Gene Expression ◽  
Early Pregnancy ◽  
Regulatory Protein ◽  
Hydroxysteroid Dehydrogenase ◽  
Steroidogenic Acute Regulatory Protein ◽  
Side Chain ◽  
Chain Cleavage ◽  
Dehydrogenase Gene ◽  
Cleavage Enzyme ◽  
Steroidogenic Acute Regulatory

scholarly journals Growth Differentiation Factor 9 Reverses Activin A Suppression of Steroidogenic Acute Regulatory Protein Expression and Progesterone Production in Human Granulosa-Lutein Cells

2010 ◽  
Vol 24 (8) ◽  
pp. 1676-1677
Author(s):  
Feng-Tao Shi ◽  
Anthony P. Cheung ◽  
Christian Klausen ◽  
He-Feng Huang ◽  
Peter C. K. Leung
Keyword(s):  
Regulatory Protein ◽  
Hydroxysteroid Dehydrogenase ◽  
Activin A ◽  
Inhibin B ◽  
Steroidogenic Acute Regulatory Protein ◽  
Side Chain ◽  
Progesterone Production ◽  
Chain Cleavage ◽  
Cleavage Enzyme ◽  
Steroidogenic Acute Regulatory

Abstract Background: We have reported that growth differentiation factor (GDF) 9 can enhance activin A (βAβA)-induced inhibin B (αβB) secretion in human granulosa-lutein (hGL) cells, but its effects on steroidogenic acute regulatory protein (StAR), ovarian steroidogenic enzymes, and progesterone production are unknown. We undertook this study to further evaluate GDF9 in this regard. Methods: hGL cells from women undergoing in vitro fertilization treatment were cultured with and without small interfering RNA (siRNA) transfection targeted at inhibin α-subunit or GDF9 before treatment with GDF9, activin A, FSH, or combinations. We compared StAR, P450 side-chain cleavage enzyme, and 3β-hydroxysteroid dehydrogenase expression in hGL cells and progesterone levels in culture media after these treatments. mRNA, protein, and hormone levels were assessed with real-time RT-PCR, immunoblotting, and ELISA, respectively. Data were analyzed by ANOVA followed by Tukey’s test. Results: Activin A alone reduced basal and FSH-induced progesterone production by decreasing the expression of StAR protein, which regulates the rate-limiting step in steroidogenesis but not P450 side-chain cleavage enzyme and 3β-hydroxysteroid dehydrogenase. GDF9 attenuated these activin A effects on StAR and progesterone. After transfection of a-subunit siRNA, activin A level increased (P < 0.001), whereas basal and activin A-induced inhibin B levels (with and without GDF9) decreased. Furthermore, the effects of GDF9 in reversing activin A suppression of progesterone production were attenuated (P < 0.001). Transfection of GDF9 siRNA decreased GDF9 as expected and led to lower StAR expression and progesterone secretion than those observed with activin A treatment alone. Conclusion: GDF9 attenuates the suppressive effects of activin A on StAR expression and progesterone production by increasing the expression of inhibin B, which acts as an activin A competitor.

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