scholarly journals 175 Effects of Bacillus spp. probiotics on growth performance, diarrhea, and systemic immunity of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli.

2018 ◽  
Vol 96 (suppl_3) ◽  
pp. 297-297
Author(s):  
Y He ◽  
K Kim ◽  
C Jinno ◽  
Z Wu ◽  
R Whelan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Enterotoxigenic Escherichia Coli ◽  
Weaned Pigs ◽  
Systemic Immunity ◽  
Bacillus Spp

478 Dietary Butyrate and Valerate Glycerides: Effects on Growth and Systemic Immunity of Weanling Piglets Coinfected with F4 and F18 Escherichia coli

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 211-212
Author(s):  
Lauren L Kovanda ◽  
Jungjae Park ◽  
Yijie He ◽  
Sangwoo Park ◽  
Ruochen Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Block Design ◽  
Enterotoxigenic Escherichia Coli ◽  
Post Inoculation ◽  
Weaned Pigs ◽  
Fecal Shedding ◽  
Systemic Immunity ◽  
Randomized Complete Block Design ◽  
Potential Benefits ◽  
Weanling Piglets

Abstract Enterotoxigenic Escherichia coli (ETEC) F4 and F18 are the two most dominant pathogenic strains in weaned pigs. The objective of this experiment was to test the effects of dietary monobutyrin and monovalerin on performance and systemic immunity of weanling piglets coinfected with F4/F18 ETEC. Twenty weaned pigs (8.21 ± 1.23 kg) were individually housed and were randomly allotted to one of three diets: control (n = 6), 0.1% monobutyrin (n = 7), or 0.1% monovalerin (n = 7). The experiment was conducted 14 days, including 7 days’ adaption and 7 days post-inoculation (PI). On d 0, d 1, and d 2 PI, pigs were inoculated with 0.5 × 109 CFU/1.5 mL each of F4 and F18 ETEC for three consecutive days. Diarrhea score was recorded daily to determine frequency of diarrhea. Piglets and feeders were weighed throughout the trial to analyze growth performance. Fecal cultures from pigs on d 0, 2, and 4 PI were inspected to identify the absence or presence of hemolytic coliforms. Blood was collected on d 0, 4, and 7 PI for complete blood cells count. All data were analyzed by the Proc Mixed of SAS with randomized complete block design. Pigs supplemented with monovalerin and monobutyrin had numerically higher ADG (249 and 282 g/day) from d 0 to d 7 PI than pigs in control (198 g/day). Supplementation of monovalerin reduced (P < 0.05) frequency of diarrhea throughout the experiment. Pigs fed monovalerin had lower (P < 0.05) neutrophil counts on d 4 PI compared with control. Hemolytic coliforms were observed in all fecal cultures from d 2 and d 4 PI, confirming fecal shedding of ETEC. Results of this study indicate the potential benefits of monovalerin supplementation on performance and disease resistance of weaned pigs coinfected with F4 and F8 ETEC.

Fructooligosaccharides improve growth performance and intestinal epithelium function in weaned pigs exposed to enterotoxigenic Escherichia coli

2020 ◽  
Vol 11 (11) ◽  
pp. 9599-9612
Author(s):  
Lei Liu ◽  
Daiwen Chen ◽  
Bing Yu ◽  
Heng Yin ◽  
Zhiqing Huang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Intestinal Epithelium ◽  
Intestinal Injury ◽  
Enterotoxigenic Escherichia Coli ◽  
Weaned Pigs ◽  
Protective Potential

This study was conducted to explore the protective potential of fructooligosaccharides (FOS) against enterotoxigenic Escherichia coli (ETEC)-induced inflammation and intestinal injury in weaned pigs.

226 Soluble fiber improved growth performance of weaned pigs challenged with enterotoxigenic Escherichia coli

2017 ◽  
Vol 95 (suppl_2) ◽  
pp. 108-109
Author(s):  
Q. Li ◽  
C. L. Loving ◽  
N. K. Gabler ◽  
E. R. Burrough ◽  
J. F. Patience
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Enterotoxigenic Escherichia Coli ◽  
Soluble Fiber ◽  
Weaned Pigs

scholarly journals Fructooligosaccharides improves growth performance and intestinal epithelium function in weaned pigs exposure to Enterotoxigenic  Escherichia coli

2020 ◽  
Author(s):  
Lei Liu ◽  
daiwen Chen ◽  
Bing Yu ◽  
Yin Heng ◽  
Zhiqing Huang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Growth Performance ◽  
Intestinal Epithelium ◽  
Amino Acid Transporter ◽  
Enterotoxigenic Escherichia Coli ◽  
Villus Height ◽  
Weaned Pigs ◽  
Glucose Transporter 2 ◽  
Acid Transporter

Abstract Background This study was conducted to explore the protective potential of Fructooligosaccharides (FOS) against Enterotoxigenic Escherichia coli (ETEC)-induced inflammation and intestinal injury in weaned pigs. Methods Twenty-four weaned pigs were randomly assigned into three groups: (1) non-challenge (CON), (2) ETEC-challenge (ECON), and (3) ETEC challenge + 250 mg/kg FOS (EFOS). On day 19, non-challenged pigs were orally infused with sterilized culture while pigs in other groups were orally infused with ETEC (2.5 × 1010 colony-forming units). After 3 days, pigs were slaughtered for sample collection. Results We show that ETEC-challenged significantly reduced average daily gain (ADG); however, FOS improved the ADG (P < 0.05) and apparent digestibility of crude protein (CP), gross energy (GE), and ash in the ETEC-challenged pigs (P < 0.05). FOS reduced plasma concentrations of IL-1β and TNF-α, and elevated the concentrations of immunoglobulin A (IgA) and immunoglobulin M (IgM) (P < 0.05). Interestingly, FOS elevated villus height in duodenum, and elevated the ratio of villus height to crypt depth in the duodenum and ileum in the ETEC-challenged pigs (P < 0.05). Moreover, FOS increased lactase activity in the duodenum and ileum (P < 0.05). The activities of sucrase and alkaline phosphatase (AKP) were higher in EFOS group than in the ECON group (P < 0.05). Importantly, FOS up-regulated the expressions of critical genes in intestinal epithelium function such as zonula occludens-1 (ZO-1), L-type amino acid transporter-1 (LAT1), and cationic amino acid transporter-1 (CAT1) in the duodenum and the expressions of ZO-1 and glucose transporter-2 (GLUT2) in the jejunum (P < 0.05). FOS also up-regulated the expressions of occludin, fatty acid transporter-4 (FATP4), sodium glucose transport protein 1 (SGLT1), and GLUT2 in the ileum (P < 0.05). FOS significantly increased the concentrations of acetic acid, propionic acid and butyric acid in the cecal digesta. Additionally, FOS reduced the populations of Escherichia coli, but elevated the populations of Bacillus and Bifidobacterium in the caecal digesta (P < 0.05). Conclusions These results suggested that FOS can improve the growth performance and intestinal health in weaned pigs upon ETEC challenge, which was associated with suppressed inflammatory responses and improved intestinal epithelium functions and microbiota.

scholarly journals 131 Effects of Bacillus spp. probiotics on systemic immunity and intestinal health of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 74-75
Author(s):  
Yijie He ◽  
Kwangwook Kim ◽  
Cynthia Jinno ◽  
Zhaohai Wu ◽  
Rose Whelan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Control Diet ◽  
Enterotoxigenic Escherichia Coli ◽  
Jejunal Mucosa ◽  
Post Inoculation ◽  
Intestinal Health ◽  
Weaned Pigs ◽  
Systemic Immunity ◽  
E Coli ◽  
Bacillus Spp

Abstract The experiment was conducted to investigate the effects of Bacillus spp. on systemic immunity and intestinal health of weaned pigs infected with F18 E. coli. Weaned pigs (n = 36, 7.61 ± 0.40 kg BW) were randomly allotted to one of three treatments: a control diet and two diets supplemented with 500 mg/kg of Bacillus spp. strain 1 (PRO1) or strain 2 (PRO2). The experiment was conducted for 28 d, including 7 d before and 21 d after the first E. coli inoculation (d 0). The doses of F18 E. coli inoculum were 1010 cfu/3 mL oral dose daily for 3 consecutive days. Serum samples were collected on d 0 before inoculation, and d 3, 7, 14, and 21 post-inoculation (PI) to measure inflammatory meditators. All pigs were euthanized on d 21 PI to collect tissue samples for gut morphology, bacterial translocation, and gene expression analysis. Data were analyzed using the Mixed Procedure of SAS. Pigs in PRO1 had reduced (P < 0.05) serum haptoglobin on d 3 and d 6 PI compared with pigs in control. Supplementation of PRO1 or PRO2 reduced (P < 0.05) total coliforms in mesenteric lymph nodes on d 21 PI. Pigs in PRO1 had greater (P < 0.05) ileal villi height than pigs in control. Pigs in PRO2 had greater (P < 0.05) sulfomucin percentage in duodenal villi and greater (P < 0.05) sialomucin percentage in jejunal villi than pigs in control. Supplementation of PRO1 also up-regulated (P < 0.05) MUC2 gene expression in jejunal mucosa but reduced (P < 0.05) COX2 and IL1B gene expression in ileal mucosa on d 21 PI, compared with control. In conclusion, supplementation of Bacillus spp. may reduce systemic inflammation and enhance intestinal health of weaned pigs infected with F18 E. coli.

scholarly journals Comparative Evaluation of the Antimicrobial and Mucus Induction Properties of Selected Bacillus Strains against Enterotoxigenic Escherichia coli

Antibiotics ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 849
Author(s):  
Natalia Bravo Santano ◽  
Erik Juncker Boll ◽  
Lena Catrine Capern ◽  
Tomasz Maciej Cieplak ◽  
Enver Keleszade ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Functional Foods ◽  
Animal Health ◽  
Enterotoxigenic Escherichia Coli ◽  
Mucus Production ◽  
Ht29 Cells ◽  
Bacillus Spp ◽  
Mucin Release ◽  
Strain Dependent ◽  
Human Intestinal Cells

Probiotics have been shown to bind to host receptors, which are important for pathogen adhesion and induce the host’s production of defence factors. They can activate the goblet-cell-derived production of mucins, a major component of the mucus layer and a physical barrier participating in limiting the proximity of microorganisms to the epithelial layer. In the last decade, Bacillus spp. strains have gained interest in human and animal health due to their tolerance and stability under gastrointestinal tract conditions. Moreover, Bacillus spp. strains can also produce various antimicrobial peptides that can support their use as commercial probiotic supplements and functional foods. The present study aimed to evaluate and determine the ability of selected Bacillus spp. strains to inhibit the growth of enterotoxigenic Escherichia coli (ETEC) F4 and to reduce binding of ETEC F4 to HT29-16E (mucus-secreting and goblet-like) human intestinal cells. Moreover, mucus production in the HT29 cells in the presence of the Bacillus spp. strains was quantified by ELISA. Bacillus spp. strains (CHCC 15076, CHCC 15516, CHCC 15541, and CHCC 16872) significantly inhibited the growth of ETEC F4. Moreover, the ability of the probiotic Bacillus spp. strains to stimulate mucin release was highly strain dependent. The treatment with Bacillus subtilis CHCC 15541 resulted in a significant increase of both MUC2 and MUC3 in HT29-16E cells. Therefore, this strain could be an up-and-coming candidate for developing commercial probiotic supplements to prevent infections caused by ETEC F4 and, potentially, other pathogens.

Effects of dietary β-glucan supplementation on growth performance and immunological and metabolic parameters of weaned pigs administered with Escherichia coli lipopolysaccharide

2018 ◽  
Vol 9 (6) ◽  
pp. 3338-3343 ◽  
Author(s):  
Cheng Wu ◽  
Qin Xu ◽  
Ru Wang ◽  
Linlin Qin ◽  
Xie Peng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Blood Parameters ◽  
Metabolic Parameters ◽  
Weaned Pigs ◽  
Escherichia Coli Lipopolysaccharide

The objective of this study was to determine the effects of dietary β-glucan (BG) supplementation on growth performance and blood parameters in weaned pigs administered with Escherichia coli lipopolysaccharide (LPS).

Sign in / Sign up

Export Citation Format

Share Document