scholarly journals New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw1

2018 ◽  
Vol 96 (9) ◽  
pp. 3928-3942 ◽  
Author(s):  
Gabriel O Ribeiro ◽  
Ajay Badhan ◽  
Jiangli Huang ◽  
Karen A Beauchemin ◽  
Wenzhu Yang ◽  
...  
Keyword(s):  
Fibrolytic Enzymes

scholarly journals Exogenous fibrolytic enzymes and recombinant bacterial expansins synergistically improve hydrolysis and in vitro digestibility of bermudagrass haylage

2019 ◽  
Vol 102 (9) ◽  
pp. 8059-8073 ◽  
Author(s):  
A.A. Pech-Cervantes ◽  
I. Muhammad ◽  
I.M. Ogunade ◽  
Y. Jiang ◽  
D.H. Kim ◽  
...  
Keyword(s):  
In Vitro Digestibility ◽  
Fibrolytic Enzymes

Influence of supplementary fibrolytic enzymes on the fermentation of corn and grass silages by mixed ruminal microorganisms in vitro.

2001 ◽  
Vol 79 (7) ◽  
pp. 1905 ◽  
Author(s):  
R J Wallace ◽  
S J Wallace ◽  
N McKain ◽  
V L Nsereko ◽  
G F Hartnell
Keyword(s):  
Fibrolytic Enzymes ◽  
Ruminal Microorganisms

scholarly journals Fibrolytic enzymes improve the nutritive value of high-moisture corn for finishing bulls

2020 ◽  
Vol 98 (2) ◽  
Author(s):  
Pedro Augusto Ribeiro Salvo ◽  
Viviane C Gritti ◽  
João Luiz Pratti Daniel ◽  
Leandro S Martins ◽  
Fernanda Lopes ◽  
...  
Keyword(s):  
Feed Efficiency ◽  
Nutritive Value ◽  
Energy Availability ◽  
Vitamin Supplement ◽  
Animal Performance ◽  
High Moisture ◽  
Fibrolytic Enzymes ◽  
High Moisture Corn

Abstract Exogenous fibrolytic enzymes (EFE) improve the energy availability of grains for nonruminant animals by reducing encapsulation of the endosperm nutrients within grain cell walls; however, these benefits are unknown in the treatment of corn-based silage for cattle. The objective of the present study was to evaluate the effects of adding EFE at ensiling on the nutritive value of high-moisture corn (HMC) and snaplage (SNAP) for finishing Nellore bulls. The EFE dose was 100 g/Mg fresh matter in both HMC and SNAP. Diets were 1) a SNAP + HMC control (without enzyme addition); 2) SNAP + HMC EFE (with enzymes); 3) a whole-plant corn silage (WPCS) + HMC control (without enzyme addition); and 4) WPCS + HMC EFE (with enzymes). In addition to the silages, the diets were also composed of soybean hulls, soybean meal, and mineral–vitamin supplement. The statistical design was a randomized complete block with a factorial arrangement of treatments, and the experiment lasted 122 d. For in situ and in vitro analyses, 2 cannulated dry cows were used. There was no interaction between the diets and EFE application (ADG, P = 0.92; DMI, P = 0.77; G:F, P = 0.70), and there was no difference between the SNAP and WPCS diets regarding the DMI (P = 0.53), ADG (P = 0.35), and feed efficiency (ADG:DMI, P = 0.83). Adding EFE to the HMC and SNAP at ensiling did not affect ADG but decreased DMI (P = 0.01), resulting in greater feed efficiency by 5.91% (P = 0.04) than that observed in animals fed diets without the addition of EFE. Addition of EFE to HMC resulted in reduced NDF content and increased in vitro and in situ DM digestibility compared with untreated HMC. No effects were found for the addition of EFE to SNAP. Fecal starch decreased with EFE application (P = 0.05). Therefore, the diet energy content (TDN, NEm, and NEg) calculated from animal performance increased (P = 0.01) with the addition of EFE to HMC. In conclusion, exchanging the NDF from WPCS with that from SNAP did not affect the performance of finishing cattle, whereas the addition of EFE to HMC at ensiling improved animal performance by increasing the energy availability of the grain.

scholarly journals Nutritional value, fermentation losses and aerobic stability of elephant grass (Pennisetum purpureum Schum.) silage treated with exogenous fibrolytic enzymes

2020 ◽  
Vol 42 ◽  
pp. e48272
Author(s):  
Maikon Figueredo Lemos ◽  
Alexandro Pereira Andrade ◽  
Pedro Henrique Ferreira da Silva ◽  
Camila Oliveira Santos ◽  
Caio Felipe Barros Souza ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Nutritional Value ◽  
Pennisetum Purpureum ◽  
Elephant Grass ◽  
Fibrolytic Enzymes ◽  
Grass Silage ◽  
Aerobic Stability ◽  
Pennisetum Purpureum Schum

The aim of this study was to evaluate nutritional value, fermentation losses, and aerobic stability of elephant grass silage (Pennisetum purpureum Schum.) treated with exogenous fibrolytic enzymes. The experiment was conducted in a completely randomized design with four replicates (experimental silos) and five levels of fibrolytic enzymes (0, 1.5, 3.0, 4.5 and 6.0%). For this, the elephant grass was ensiled at 70 days of age in plastic buckets with 20L capacity. Silos were opened 60 days after sealing. Analyses were made for chemical composition, in vitro dry matter digestibility (IVDMD), effluent losses (EL), gas losses (GL) and dry matter recovery (DMR), as well as the aerobic stability of the silage. Data were analyzed with PROC REG of SAS® University, at 5% probability. There was an increase in IVDMD content (p < 0.0001) and reduction in NDF and ADF contents (p < 0.0001) according to enzyme levels. These results were related to the increase in the degradation of fiber fractions. There were higher EL (p = 0.0062) as a function of enzyme levels and aerobic deterioration after silo opening, at all levels tested. Thus, it can be concluded that the exogenous fibrolytic enzymes change the chemical composition of elephant grass silage, and increase its digestibility and nutritional value. Moreover, when used alone as an additive, fibrolytic enzymes are not able to recover all dry matter of this silage (with effluent and gas losses), and are not able to maintain aerobic stability in the first hours after opening the silos.

scholarly journals In vitro degradability of coffee husks treated with doses of fibrolytic enzymes for use in ruminant nutrition

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2691
Author(s):  
Yasmin Haluan Porto Moura ◽  
Carmen Lucia De Souza Rech ◽  
Mauro Pereira de Figueiredo ◽  
José Luiz Rech ◽  
Yann Dos Santos Luz ◽  
...  
Keyword(s):  
Dry Matter ◽  
Animal Feed ◽  
Neutral Detergent Fiber ◽  
Agricultural Activity ◽  
State University ◽  
Fibrolytic Enzymes ◽  
Coffee Husk ◽  
Randomized Design ◽  
Ruminal Degradability

The agricultural activity has generated a progressive amount of waste, which needs a proper treatment to avoid negative environmental impacts. At the same time, values can be added to such waste, as its use in animal feed. This research was conducted at the laboratory of Animal Nutrition, State University of Southwestern Bahia, campuses of Vitória da Conquista and Itapetinga. The objective of this study was to evaluate the effect of coffee husks on ruminant feeds by increasing doses of fibrolytic enzymes, evaluating their effects on in vitro ruminal degradability of dry matter (DM), neutral detergent fiber (NDF), and acid detergent fiber (FDA) of the coffee husk (CH). The experiment was a completely randomized design in a 2x4 factorial scheme. It compounded the following treatments: Coffee husk (CH1): 1.5% enzymes (E) and 24 h enzymatic action (EA); CH2: 3.0% (E) and 24h (EA); CH3: 4.5% (E) and 24 h (EA); CH4: 6% (E) and 24 h (EA); CH5: 1.5% (E) 48 h (EA); CH6: 3% (E) and 48h (EA); CH7: 4.5% (E) and 48h (EA); and CH8: 6% (E) and 48 h (EA), all based on dry matter. An improvement in the degradability of the nutritional parameters MS, NDF, and FDA occurred with the addition of enzymes, with 3% enzyme addition being the best level, and 24 hours, as the best action time. In addition to that, as the EA on coffee husk increased, the degradation rate decreased. Therefore, the use of enzymes can improve the digestibility of the fibrous fraction, enabling the use of the coffee husk and possibly other agroindustrial residues, thus minimizing their adverse effects on nature.

scholarly journals Effects of fibrolytic enzymes and lactic acid bacteria on fermentation quality and in vitro digestibility of Napier grass silage

2019 ◽  
Vol 18 (1) ◽  
pp. 1438-1444 ◽  
Author(s):  
Smerjai Bureenok ◽  
Sioudome Langsoumechai ◽  
Nittaya Pitiwittayakul ◽  
Chalermpon Yuangklang ◽  
Kraisit Vasupen ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Napier Grass ◽  
In Vitro Digestibility ◽  
Fibrolytic Enzymes ◽  
Grass Silage ◽  
Fermentation Quality

scholarly journals In vitro gas production of foliage from three browse tree species treated with different dose levels of exogenous fibrolytic enzymes

2016 ◽  
Vol 100 (5) ◽  
pp. 920-928 ◽  
Author(s):  
D. López ◽  
J. F. Vázquez-Armijo ◽  
N. López-Villalobos ◽  
H. A. Lee-Rangel ◽  
A. Z. M. Salem ◽  
...  
Keyword(s):  
Tree Species ◽  
Gas Production ◽  
Fibrolytic Enzymes ◽  
In Vitro Gas Production ◽  
Dose Levels

Fiber degradability, chemical composition and conservation characteristics of alfalfa haylage ensiled with exogenous fibrolytic enzymes and a ferulic acid esterase-producing inoculant

2014 ◽  
Vol 94 (4) ◽  
pp. 697-704 ◽  
Author(s):  
J. P. Lynch ◽  
D. Prema ◽  
J. D. Van Hamme ◽  
J. S. Church ◽  
K. A. Beauchemin
Keyword(s):  
Chemical Composition ◽  
Ferulic Acid ◽  
Untreated Control ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
Ferulic Acid Esterase ◽  
Acid Detergent Fiber ◽  
Fibrolytic Enzymes ◽  
Effect Of Treatment

Lynch, J. P., Prema, D., Van Hamme, J. D., Church, J. S. and Beauchemin, K. A. 2014. Fiber degradability, chemical composition and conservation characteristics of alfalfa haylage ensiled with exogenous fibrolytic enzymes and a ferulic acid esterase-producing inoculant. Can. J. Anim. Sci. 94: 697–704. This study investigated the effects of two fibrolytic enzyme products, applied at baling alone or in combination with a ferulic acid esterase-producing bacterial additive, on the ensilage dynamics, chemical composition and digestibility of alfalfa haylage. Five replicate wrapped bales were produced with one of five treatments, including an untreated control, and one of two fibrolytic enzyme products (EN1 and EN2) applied either alone or in combination with a ferulic-acid producing bacterial additive (FAEI). No effect of treatment was observed on the neutral detergent fiber (NDF) (P=0.889) or acid detergent fiber (ADF) (P=0.065) concentrations of haylage after ensilage, but haylage produced using fibrolytic enzyme products underwent greater (P<0.018) increases in temperature following exposure to aerobic conditions. Haylages produced with fibrolytic enzyme products had a greater (P<0.001) in vitro NDF degradability (NDFD) than untreated haylage. The use of fibrolytic enzymes applied to alfalfa haylage at ensiling increased the NDFD, despite minimal effects on the chemical composition of the herbage. However, the greater aerobic deterioration of fibrolytic enzyme-treated bales indicates higher dry matter losses during aerobic exposure. The use of FAEI with fibrolytic enzymes did not further enhance the effects of fibrolytic-enzyme treatments.

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