scholarly journals Influence of bull age, ejaculate number, and season of collection on semen production and sperm motility parameters in Holstein Friesian bulls in a commercial artificial insemination centre

2018 ◽  
Vol 96 (6) ◽  
pp. 2408-2418 ◽  
Author(s):  
Edel M Murphy ◽  
Alan K Kelly ◽  
Ciara O’Meara ◽  
Bernard Eivers ◽  
Patrick Lonergan ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Holstein Friesian ◽  
Motility Parameters

scholarly journals Effect of Post -Thaw Addition of Insemination Media and Prostatic Fluid on Dog Sperm Motility Parameters

2018 ◽  
Vol 75 (2) ◽  
pp. 236
Author(s):  
Violeta IGNA ◽  
Ramona FENEȘ ◽  
Ileana BRUDIU
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Sperm Parameters ◽  
Sperm Velocity ◽  
Progressive Motility ◽  
Prostatic Fluid ◽  
Current Requirement ◽  
Negative Effect ◽  
A Current ◽  
Motility Parameters

Improving sperm parameters with a role in fertilization and keeping them as long as possible at optimal levels is still a current requirement for frozen-thawed semen. The aim of the study was to evaluate the effect of the addition of an artificial insemination media (M) and prostatic fluid (PF), post thawing, on dog sperm motility parameters. The sperm rich fraction from nine dog ejaculates was cryopreserved. After thawing, three experimental variants were carried out: 1) semen diluted 1:1 with CaniPlus Enhance–media 2) with the PF and 3) semen without any addition. Samples were incubated at 37°C and sperm motility parameters were assessed after 10, 25, 40 and 50 minutes. Samples supplemented with the insemination media recorded the highest values of total motility, progressive motility and sperm velocity throughout incubation. Addition of PF had a negative effect on the total and progressive motility values and caused a slight increase in velocity.

scholarly journals Influence of Coconut Powder Water-Based Conservation Medium (APC-102c) for Maintaining Mitochondrial Activity of Cryopreserved Ram Sperm

2019 ◽  
Vol 47 (1) ◽  
Author(s):  
Bruna Farias Brito ◽  
Bárbara Mara Bandeira Santos ◽  
Leonardo Alves Rodrigues Cabral ◽  
David Baruc Cruvinel Lima ◽  
Cristiane Clemente De Melo Salgueiro ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Mitochondrial Activity ◽  
Plasma Membrane Integrity ◽  
Significant Difference ◽  
Motility Parameters

Background:  Semen extenders are required to protect and preserve semen, and the development of suitable extenders is key for artificial insemination. Although the use of Tris-based diluent is widespread, new diluents such as powdered coconut water have been developed for better sperm protection. One way to evaluate the effectiveness of diluents is through microscopic analyses that evaluate sperm motility, vigor, and concentration. However, these analyses are limited, and may not provide accurate results. New evaluation techniques have been studied, and one of the tests that can be used to add reliability to these analyses is mitochondrial activity evaluation, which can sum all the parameters, and provide a more accurate evaluation. Thus, the present study aimed to evaluate the efficacy of ACP-102c in cryopreserved ram semen.Materials, Methods & Results: Five semen samples were collected from two ram breeders using artificial vagina (n = 10). Each ejaculate was divided into the following two treatments: T1 - ACP-102c + 20% egg yolk + 7% glycerol and T2 - TRIS + 20% egg yolk + 7% glycerol. Extended semen samples were then packed in 0.5 mL plastic straws, subjected through the refrigeration curve up to 4°C (0.35° C/min), and equilibrated for 2 h at 4°C. Subsequently, the straws were placed at 4 cm above liquid nitrogen level (-60°C) for 15 min, immersed, and then finally stored in the liquid nitrogen at -196°C. Both fresh and thawed samples were evaluated for total and progressive sperm motility using conventional microscopy (40x), and the same evaluator on each occasion. For plasma membrane integrity (IMP), the smear staining technique with the Eosin-Nigrosin staining was used; 200 sperms were counted and classified as whole (unstained) and unhealthy (stained). Mitochondrial activity was evaluated using a cytochemical technique based on the oxidation of 3,3'-diaminobenzidine (DAB); 200 sperms were counted, and classified into four classes (I, II, III, and IV) according to the degree of coloration of the intermediate part. Fresh semen showed no significant difference (P > 0.05) between treatments with respect to motility parameters; however, T2 showed significantly inferior results regarding plasma membrane integrity. After thawing, T2 was significantly higher in sperm motility parameters compared to T1. The mitochondrial activity and plasma membrane integrity parameters did not show any significant difference between the treatments.Discussion: The TRIS-based diluent showed higher motility values than ACP-102c; however, motility rates in ACP-102c diluent, although lower, are considered satisfactory for insemination, which requires semen with minimal progressive motility of 30%. Notably, the cryopreservation protocol used in this study is the standard for TRIS-based diluent, and it is known that the optimal rate of refrigeration and cryopreservation may differ according to the composition of the storage medium; therefore, we may assume that the protocol used is not yet appropriate for the ACP-102c diluent, and further studies are required. IMP is an essential attribute for fertilization, and cryopreservation can affect the plasma membrane as observed in this study. Cryopreserved semen reduced the percentage of class I mitochondrial reaction sperms in both treatments, demonstrating that cryopreservation affects the mitochondrial activity of the intermediate portion of the sperm; however, there was no difference between treatments in thawed semen. Thus, we concluded that the ACP-102c conservation medium maintains seminal quality after thawing, and it can be used in artificial insemination processes.

Comparison of Computer-assisted Sperm Motility Analysis Parameters in Semen from Belgian Blue and Holstein?Friesian Bulls

2007 ◽  
Vol 42 (2) ◽  
pp. 153-161 ◽  
Author(s):  
G Hoflack ◽  
G Opsomer ◽  
T Rijsselaere ◽  
A Van Soom ◽  
D Maes ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Computer Assisted ◽  
Holstein Friesian

Cooled semen for fixed-time artificial insemination in beef cattle

2016 ◽  
Vol 28 (7) ◽  
pp. 1004 ◽  
Author(s):  
Juliana C. Borges-Silva ◽  
Márcio R. Silva ◽  
Daniel B. Marinho ◽  
Eriklis Nogueira ◽  
Deiler C. Sampaio ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Egg Yolk ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Reproductive Efficiency ◽  
Sperm Abnormalities ◽  
Hypoosmotic Swelling Test ◽  
Interesting Approach

This study evaluated the use of cooled semen in a fixed-time artificial insemination (FTAI) program compared with frozen–thawed semen to improve pregnancy rates in beef cattle. Ejaculates of three bulls were collected and divided into two treatments: (1) frozen–thawed semen and (2) cooled semen. Egg-yolk extender without glycerol was used for the cooled semen treatment. Straws (25 × 106 spermatozoa) were submitted to cooling for preservation at 5°C for 24 h, after which FTAI was performed. Nelore cows (n = 838) submitted to FTAI were randomly inseminated using frozen–thawed semen or cooled semen. There was a 20% increase in the pregnancy per AI (P AI–1) using cooled semen compared with frozen–thawed semen (59.9 ± 4.7 vs 49.4 ± 5.0%; P < 0.005). There was no difference in P AI–1 among the bulls (P = 0.40). The frozen–thawed semen had fewer functional spermatozoa than did the cooled semen when evaluated by sperm motility (61.7 vs 81.0%), slow thermoresistance test (41.7 vs 66.7%) and hypoosmotic swelling test (38.3 vs 53.7%; P < 0.05). The percentage of sperm abnormalities did not differ between the freeze–thawing and cooling processes (18.6 vs 22.1%; P > 0.05). Because there was less damage to spermatozoa and improvement in P AI–1, the use of cooled semen instead of frozen–thawed semen is an interesting approach to increase reproductive efficiency in cattle submitted to a FTAI protocol.

Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus

2017 ◽  
Vol 29 (7) ◽  
pp. 1319 ◽  
Author(s):  
Olga Bondarenko ◽  
Borys Dzyuba ◽  
Marek Rodina ◽  
Jacky Cosson
Keyword(s):  
Sperm Motility ◽  
Seminal Fluid ◽  
Mature Male ◽  
Sperm Maturation ◽  
Wolffian Duct ◽  
Testicular Spermatozoa ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus ◽  
Motility Parameters

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.

scholarly journals Characteristics of Siberian sturgeon and sterlet sperm motility parameters compared using CASA

2012 ◽  
Vol 20 (2) ◽  
Author(s):  
Piotr Sieczyński ◽  
Jan Glogowski ◽  
Beata Cejko ◽  
Cezary Grygoruk
Keyword(s):  
Sperm Motility ◽  
Siberian Sturgeon ◽  
Motility Parameters

Effect of albumin and casein supplementation on the common carp Cyprinus carpio L. sperm motility parameters measured by CASA

2013 ◽  
Vol 22 (1) ◽  
pp. 123-129 ◽  
Author(s):  
Radosław Kajetan Kowalski ◽  
Beata Irena Cejko ◽  
Sławomir Krejszeff ◽  
Beata Sarosiek ◽  
Sylwia Judycka ◽  
...  
Keyword(s):  
Common Carp ◽  
Sperm Motility ◽  
Cyprinus Carpio ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
The Common ◽  
Motility Parameters

scholarly journals Qualities of goat semen in Tris-Citrate-Glucose extender containing glutathione

1970 ◽  
Vol 27 (2) ◽  
pp. 46-55 ◽  
Author(s):  
B Mishra ◽  
MGS Alam ◽  
MAMY Khandokar ◽  
S Mazumder ◽  
MN Munsi
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Phase Contrast ◽  
Phase Contrast Microscope ◽  
Contrast Microscope ◽  
And Control ◽  
Control Samples

Glutathione (GSH) 0 (control), 2, 4 and 8 mM was used in the preservation of chilled goat semen. Treated and control samples were kept at 4 – 5°C up to seven days. Sperm motility and acrosome abnormality were assessed daily under phase contrast microscope. The sperm motility was significantly (P<0.01) higher in the semen treated with 8 mM GSH. Optimum sperm motility (≥50%) for artificial insemination was retained for three days with 2 and 4 mM GSH and up to four days with 8 mM GSH. Acrosomal damage was significantly (P<0.01) reduced to ≤ 1.0% after addition of 8 mM GSH. It is suggested that GSH may be used as an antioxidant for better preservation of goat semen for artificial insemination. DOI: 10.3329/bvet.v27i2.7554 Bangl. vet. 2010. Vol. 27, No. 2, 46 – 55

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