27 Composition and in Situ Gene Expression of Rumen Wall Microbial Communities.

2018 ◽  
Vol 96 (suppl_2) ◽  
pp. 14-15
Author(s):  
S Schmitz-Esser
Keyword(s):  
Gene Expression ◽  
Microbial Communities ◽  
In Situ Gene Expression

In Situ Gene Expression in Mixed-Culture Biofilms: Evidence of Metabolic Interactions between Community Members

1998 ◽  
Vol 64 (2) ◽  
pp. 721-732 ◽  
Author(s):  
Søren Møller ◽  
Claus Sternberg ◽  
Jens Bo Andersen ◽  
Bjarke Bak Christensen ◽  
Juan Luis Ramos ◽  
...  
Keyword(s):  
Gene Expression ◽  
Microbial Communities ◽  
Pure Culture ◽  
Fluorescent Protein ◽  
Confocal Laser ◽  
Specific Gene ◽  
Community Members ◽  
Metabolic Interactions ◽  
Green Fluorescent

ABSTRACT Microbial communities growing in laboratory-based flow chambers were investigated in order to study compartmentalization of specific gene expression. Among the community members studied, the focus was in particular on Pseudomonas putida and a strain of anAcinetobacter sp., and the genes studied are involved in the biodegradation of toluene and related aromatic compounds. The upper-pathway promoter (Pu) and themeta-pathway promoter (Pm) from the TOL plasmid were fused independently to the gene coding for the green fluorescent protein (GFP), and expression from these promoters was studied inP. putida, which was a dominant community member. Biofilms were cultured in flow chambers, which in combination with scanning confocal laser microscopy allowed direct monitoring of promoter activity with single-cell spatial resolution. Expression from thePu promoter was homogeneously induced by benzyl alcohol in both community and pure-culture biofilms, while the Pmpromoter was induced in the mixed community but not in a pure-culture biofilm. By sequentially adding community members, induction ofPm was shown to be a consequence of direct metabolic interactions between an Acinetobacter species and P. putida. Furthermore, in fixed biofilm samples organism identity was determined and gene expression was visualized at the same time by combining GFP expression with in situ hybridization with fluorescence-labeled 16S rRNA targeting probes. This combination of techniques is a powerful approach for investigating structure-function relationships in microbial communities.

The endothelin system in human and monkey ovaries: in situ gene expression of the different components

1999 ◽  
Vol 295 (1) ◽  
pp. 101-109 ◽  
Author(s):  
Habib Karam ◽  
Olivier Valdenaire ◽  
Marie-France Belair ◽  
Caroline Prigent-Sassy ◽  
Annette Rakotosalama ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelin System ◽  
In Situ Gene Expression

scholarly journals Analyzing in situ gene expression in the mouse brain with image registration, feature extraction and block clustering

2007 ◽  
Vol 8 (Suppl 10) ◽  
pp. S5 ◽  
Author(s):  
Manjunatha Jagalur ◽  
Chris Pal ◽  
Erik Learned-Miller ◽  
R Thomas Zoeller ◽  
David Kulp
Keyword(s):  
Gene Expression ◽  
Feature Extraction ◽  
Image Registration ◽  
Mouse Brain ◽  
Block Clustering ◽  
In Situ Gene Expression
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