scholarly journals Influence of feeding thermally peroxidized soybean oil on growth performance, digestibility, gut integrity, and oxidative stress in nursery pigs

2020 ◽  
Vol 98 (2) ◽  
Author(s):  
Brian J Kerr ◽  
Stephanie C Lindblom ◽  
Martin F Overholt
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Soybean Oil ◽  
Growth Performance ◽  
Nursery Pigs ◽  
Lipid Peroxidation Products ◽  
Thermally Processed ◽  
Markers Of Oxidative Stress ◽  
Effect Of Feeding ◽  
Lipid Digestibility

Abstract The objectives of the current experiments were to evaluate the effect of feeding soybean oil (SO) with different levels of peroxidation on lipid, N, and GE digestibility, gut integrity, oxidative stress, and growth performance in nursery pigs. Treatments consisted diets containing 10% fresh SO (22.5 °C) or thermally processed SO (45 °C for 288 h, 90 °C for 72 h, or 180 °C for 6 h), each with an air infusion of 15 L/min, with postprocessing peroxide values of 7.6, 11.5, 19.1, and 13.4 mEq/kg and p-anisidine values of 1.92, 6.29, 149, and 159, for the 22.5 °C, 45 °C, 90 °C and 180 °C processed SO, respectively. In experiment 1, 64 barrows (7.1 ± 0.9 kg initial BW) were randomly allotted into 2 rooms of 32 pens and individually fed their experimental diets for 21 d, with a fresh fecal sample collected on day 20 for determination of GE and lipid digestibility. In experiment 2, 56 barrows (BW 9.16 ± 1.56 kg) were placed into individual metabolism crates for assessment of GE, lipid, and N digestibility and N retention. Urinary lactulose to mannitol ratio was assessed to evaluate in vivo small intestinal integrity, and urine and plasma were collected to analyze for markers of oxidative stress. Pigs were subsequently euthanized to obtain liver weights and analyze the liver for markers of oxidative stress. In experiment 1, pigs fed the SO thermally processed at 90 °C had reduced ADG (P = 0.01) and ADFI (P = 0.04) compared to pigs fed the other SO treatment groups, with no differences noted among pigs fed the 22.5 °C, 45 °C, and 180 °C SO treatments. No effects of feeding thermally processing SO on dietary GE or lipid digestibility (P > 0.10) were noted in either experiment. In experiment 2, there was no dietary effect of feeding peroxidized SO on the DE:ME ratio, N digestibility, or N retained as a percent of N digested, on the urinary ratio of lactulose to mannitol, on serum, urinary, or liver thiobarbituric acid reactive substances, on plasma protein carbonyls, or on urinary or liver 8-OH-2dG (P > 0.10). In experiment 2, pigs fed the SO thermally processed at 90 °C had the greatest isoprostane concentrations in the serum (P ≤ 0.01) and urine (P ≤ 0.05) compared to pigs fed the unprocessed SO. These results indicate that the change in fatty acid composition and/or the presence of lipid peroxidation products in peroxidized SO may reduce ADG and ADFI in nursery pigs, but appears to have no impact on GE, lipid, or N digestibility, or gut permeability. These data suggest that the presence of lipid peroxidation products may affect certain markers of oxidative stress.

246 Effects of Feeding Thermally Processed Spray-dried Egg Whites on Growth Performance, Apparent Digestibility, and Oxidative Stress in Nursery Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 91-92
Author(s):  
Victoria C Wilson ◽  
Brian J Kerr
Keyword(s):  
Oxidative Stress ◽  
Growth Performance ◽  
Liver Tissue ◽  
Protein Carbonyls ◽  
Nursery Pigs ◽  
Thermally Processed ◽  
Markers Of Oxidative Stress ◽  
Dietary Treatments ◽  
And Oxidative Stress ◽  
Spray Dried

Abstract The objectives of this study were to determine if feeding thermally processed (TP, heated at 100°C for 120 h) spray-dried egg whites (SDEW) to nursery pigs would impact growth performance, apparent total tract digestibility (ATTD) of GE, N, and S, and oxidative stress. Thirty-two barrows, (initial BW 7.1 kg) were randomly assigned to dietary treatments with 1 pig per pen. In a preliminary study, thermally processing SDEW at 100°C for 120 h increased protein carbonyls (PC) from 6 µmol/g to 19.4 µmol/g (P ≤ 0.01). Diets included either 12% SDEW, 6% TP-SDEW plus 6% SDEW, or 12% TP-SDEW. The experiment lasted 24 d for collection of growth performance data, while plasma was collected on d 21 and liver tissue harvested on d 24 to analyze for markers of oxidative stress. Feces were collected on d 22 for measures of ATTD. Daily gain, daily feed intake, feed efficiency, and ATTD of GE were not found to be different among dietary treatments (P ≥ 0.57). In contrast, ATTD of N (P = 0.11) and S (P = 0.03) were found to increase with increasing protein oxidation in the diet. There was no change in the plasma or liver F2-isoprostanes and 8-hydroxy-2’-deoxyguanosine among dietary treatments (P ≥ 0.28). An increase in plasma PC (P = 0.02) was observed in pigs fed 12% TP-SDEW compared to pigs fed 12% SDEW and pigs fed 6% TP-SDEW. In contrast, a decrease in liver tissue PC (P = 0.04) was observed in pigs fed 6% TP-SDEW compared to pigs fed 12% SDEW and 12% TP-SDEW. These results indicate that feeding TP-SDEW does not affect growth performance, ATTD of GE, and oxidative stress as indicated by F2-isoprostanes or 8-hydroxy-2’-deoxyguanosine; but appeared to have variable effects on oxidative stress as measured by PC.

scholarly journals Effects of Fumonisin-Contaminated Corn on Growth Performance of 9 to 28 kg Nursery Pigs

Toxins ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 604
Author(s):  
Zhong-Xing Rao ◽  
Mike D. Tokach ◽  
Jason C. Woodworth ◽  
Joel M. DeRouchey ◽  
Robert D. Goodband ◽  
...  
Keyword(s):  
Growth Performance ◽  
Linear Response ◽  
Animal Feed ◽  
Average Daily Gain ◽  
Fumonisin B1 ◽  
Nursery Pigs ◽  
Feed Production ◽  
Dietary Treatments ◽  
Daily Gain ◽  
Effect Of Feeding

Fumonisin contamination in corn is an emerging issue in animal feed production. Fumonisin disrupts the metabolism of sphingolipids and reduces growth performance. This experiment was conducted to determine the effect of feeding fumonisin-contaminated corn on growth performance and sphinganine (SA) to sphingosine (SO) ratios of 9 to 28 kg pigs. A total of 350 pigs, were used with 5 pigs/pen and 14 pens/treatment. Dietary treatments contained fumonisin-contaminated corn (50 mg/kg of fumonisin B1 + B2) blended with low fumonisin corn (10 mg/kg of fumonisin B1 + B2) to provide dietary fumonisin concentrations of 7.2, 14.7, 21.9, 32.7, and 35.1 mg/kg. From day 0 to 28, increasing fumonisin concentration decreased (linear, p < 0.001) average daily gain, average daily feed intake (linear, p = 0.055), and gain:feed ratio (linear, p = 0.016). Although these response criteria tested linear, the greatest reduction in performance was in pigs fed with 32.7 and 35.1 mg/kg of fumonisin (B1 + B2). Increasing fumonisin concentration increased the serum SA:SO ratio (linear, p < 0.001) on day 14 and 28. In summary, for 9 to 28 kg nursery pigs, increasing fumonisin linearly decreased average daily gain and gain:feed ratio. However, despite the linear response, diets containing up to 21.9 mg/kg of fumonisin did not have as dramatic a decrease in growth performance as those fed more than 32.7 mg/kg. Further research is warranted to determine the effect of fumonisin concentrations between 21.9 and 32.7 mg/kg.

scholarly journals Local and systemic oxidative stress in chronic suppurative otitis media

2021 ◽  
pp. 148-156
Author(s):  
I. D. Dubinets ◽  
M. Yu. Korkmazov ◽  
A. I. Sinitskii ◽  
E. I. Danshova ◽  
I. N. Skirpichnikov ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Surgical Treatment ◽  
Temporal Bone ◽  
Otitis Media ◽  
Local Level ◽  
Chronic Suppurative Otitis Media ◽  
Suppurative Otitis Media ◽  
Lipid Peroxidation Products ◽  
Systemic Level

Introduction. According to the literature, oxidative stress is described as one of the main factors in the pathogenesis of chronic suppurative otitis media, supporting the inflammatory process at the local level. The transition of inflammatory mediators to the systemic level is associated with the risk of developing ear purulent-destructive complications. The study of the products of lipid peroxidation in comparison with morphological changes in the structures of the temporal bone will justify the tactics of the operation.Aim. Comparison of the levels of lipid peroxidation products at the local and systemic levels in chronic suppurative otitis media, depending on the nature of pathomorphological changes in the structures of the temporal bone.Materials and methods. A prospective study of 130 patients with chronic suppurative otitis media at the age of 20-62 years with a verified diagnosis of chronic suppurative otitis media, admitted for surgical treatment, was carried out. To study the indicators of oxidative stress at the systemic level, the blood serum of patients was used; at the local level, the bone biomaterial obtained from patients during the surgical treatment of chronic suppurative otitis media was used. The quantitative determination of the primary, secondary and final products of peroxidation was carried out in the groups of patients with separate registration of lipoperoxides in the heptane and isopropanol phases of the lipid extract by spectrophotometry.Results and discussion. In the observation of patients with morphological signs of purulent destruction of the temporal bone, not only a local level of inflammation activity, but also a systemic level of an unfavorable outcome was revealed in two variants: osteoproliferation or osteonecrosis of the bone tissue of the temporal bone in chronic purulent otitis media with a constant threat to the patient's life due to intracranial purulent complications.Conclusion. The appearance in low concentrations of lipid peroxidation products in serum in patients with chronic purulent otitis media substantiates the need for a behind-the-ear approach in reconstructive-sanitizing otosurgery even with minimal clinical manifestations and CT scan data, since at the preclinical level it confirms the osteonecrotic type of bone remodeling with the risk of delayed death.

Biochemical markers of oxidative stress in maize seedlings exposed to rose-grass aphids

2021 ◽  
Vol 53 (1) ◽  
pp. 23-34
Author(s):  
I. Łukasik ◽  
S. Goławska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Anion ◽  
Biochemical Markers ◽  
Carbonyl Groups ◽  
Maize Seedlings ◽  
Aphid Infestation ◽  
Markers Of Oxidative Stress ◽  
Aphid Feeding ◽  
Superoxide Anion Radicals

We studied the influence of rose-grass aphid (Metopolophium dirhodum L.) infestation on the biochemical markers of oxidative stress in seedlings of two maize (Zea mays L.) varieties (relatively resistant Ambrozja and resistant Plomyk). We compared the generation of superoxide anion radicals O2-, level of hydrogen peroxide (H2O2), lipid peroxidation products (MDA) as well as markers of protein damage (protein-bound carbonyl groups). The studied parameters were measured at 24, 48, 72 and 96 h post-initial aphid infestation compared to the non-infested control seedlings. Our studies indicated that the rose-grass aphid feeding evoked oxidative stress in the maize seedlings. Investigated Z. mays cultivars exhibited excessive generation of superoxide anion radicals in response to insect treatments. Rose-grass aphid feeding increased the H2O2 level in maize tissues with similar levels observed at most periods post-infestation with M. dirhodum, also increased lipid peroxidation products with the maximal levels at 48 and 72 h for Ambrozja and 48, 72 and 96 h post-infestation for Plomyk varieties. Further at 48 and 72 h post-initial aphid infestation, there was an increase in protein bound carbonyl groups content in the maize seedlings after infestation with aphids.

475 The Effect of Increasing Total Sulfur Amino Acids to Lysine Level and Methionine Source on Growth Performance and Oxidative Parameters of Nursery Pigs Fed Oxidized Soybean Oil

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 211-211
Author(s):  
Jesus A Acosta ◽  
Ju Xing Chen ◽  
Deana Hancock ◽  
Mercedes Vásquez-Añón
Keyword(s):  
Amino Acids ◽  
Soybean Oil ◽  
Growth Performance ◽  
Total Antioxidant Status ◽  
Positive Control ◽  
Oxidative Status ◽  
Total Sulfur ◽  
Sulfur Amino Acids ◽  
Nursery Pigs ◽  
Oxidative Challenge

Abstract Total sulfur amino acids (TSAA): Lys level and Met source effects on growth performance and oxidative status were evaluated in nursery pigs fed oxidized soybean-oil. A total of 240 weaned pigs (BW=5.84± 0.96kg) were blocked by BW and randomly allotted to 60 pens. Treatments included a positive control containing fresh soybean-oil (PC) and 4 peroxidized soybean-oil treatments in a 2×2 factorial of 2 Met sources (DL-Met vs. 2-hydroxy-4-(methylthio)butanoate [HMTBa]) and 2 standardized ileal digestible TSAA: Lys levels (L1=0.58 vs. L2=0.65). Diets were fed for 42d. One pig per pen was euthanized at d42 for tissue analyses. Data were analyzed using PROC MIXED procedure of SAS. TSAA: Lys ratio did not affect ADG and ADFI. Met source did not affect ADG. HMTBa tended to increase ADFI compared to DL-Met (P = 0.08). There was a tendency for interaction between TSAA: Lys level and Met source for G: F (P = 0.07); pigs fed HMTBa-L1 tended to have decreased G: F compared to HMTBa-L2 and DL-Met-L1-L2. HMTBa had increased liver total antioxidant status (TAS) compared to DL-Met (P = 0.02). There was no TSAA:Lys level effect on TAS. Comparing PC to peroxidized treatments: pigs fed HMTBa-L1 and DL-Met-L1 tended to have decreased ADG (P ≤ 0.10) and DL-Met-L2 had decreased ADG (P &lt; 0.05) than PC. HMTBa-L2 was similar to PC. Pigs fed DL-Met-L1 had decreased (P &lt; 0.05) and DL-Met-L2 tended to have decreased ADFI (P ≤ 0.10) than PC. HMTBa-L1-L2 were similar to PC. All peroxidized treatments had similar G:F compared to PC. Pigs fed DL-Met-L2 tended to have decreased TAS compared to PC (P ≤ 0.10), but PC was similar to pigs fed DL-Met-L1 and HMTBa-L1-L2. In conclusion, under an oxidative challenge, HMTBa, at a TSAA: Lys ratio of 0.65, maintained nursery pigs’ growth performance and oxidative status.

Lipid Peroxidation, Protein Oxidation, Gelatinases, and Their Inhibitors in a Group of Adults with Obesity

2019 ◽  
Vol 51 (06) ◽  
pp. 389-395 ◽  
Author(s):  
Gregorio Caimi ◽  
Baldassare Canino ◽  
Maria Montana ◽  
Caterina Urso ◽  
Vincenzo Calandrino ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Protein Oxidation ◽  
Thiobarbituric Acid ◽  
Protein Carbonyl ◽  
The Body ◽  
Control Group ◽  
The Matrix ◽  
Markers Of Oxidative Stress ◽  
Obese Subjects

AbstractThe association between obesity and cardiovascular diseases has a multifactorial pathogenesis, including the synthesis of inflammatory molecules, the increase in oxidative stress and the dysregulation of the matrix metalloprotease (MMP) concentration and activity. In a group of adults with obesity, divided in 2 subgroups according to the body mass index (BMI), we examined lipid peroxidation, expressed as thiobarbituric acid-reactive substances (TBARS), protein oxidation, expressed as protein carbonyl groups (PCs), plasma gelatinases (MMP-2 and MMP-9), and their tissue inhibitors (TIMP-1 and TIMP-2). In the whole group, as well as in the 2 subgroups (with BMI 30–35 or BMI>35) of obese subjects, we observed an increase in TBARS, PCs, MMP-2, and MMP-9, and also TIMP-1 and TIMP-2 in comparison with the control group. A positive correlation between TBARS and PCs emerged in obese subjects and persisted after dividing obese subjects according to BMI. The correlation between MMP-2 and TIMP-2 was not statistically significant, while a significant correlation was present between MMP-9 and TIMP-1. The correlations between the markers of oxidative stress (TBARS and PCs) and those of the MMP/TIMP profile indicated a more marked influence of protein oxidation on MMPs and TIMPs in comparison with TBARS. The innovative aspect of our study was the simultaneous evaluation of oxidative stress markers and MMP/TIMP profile in adult obese subjects. We observed significant alterations and correlations that may negatively influence the clinical course of the disease.

Antioxidant Responses and Lipid Peroxidation of Penaeus Indicus Postlarvae Subjected to Sublethal Copper Exposure

Crustaceana ◽  
2011 ◽  
Vol 84 (10) ◽  
pp. 1197-1210 ◽  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Copper Toxicity ◽  
Sod Activity ◽  
Lipid Peroxidation Products ◽  
Coastal Marine ◽  
Potential Biomarker ◽  
Penaeus Indicus

AbstractThe objective of this study was to determine the effect of sublethal copper concentrations on certain antioxidant enzymes and lipid peroxidation products in the postlarvae (PL) of Penaeus indicus when subjected to short- and long-term exposure in the laboratory. The PL of P. indicus were exposed to 0.1641 ppm (sublethal) copper for a period of 30 days along with a parallel control. Sampling was carried out at six different time intervals, i.e., 24, 48, and 96 hrs (shortterm), and 10, 20, and 30 days (long-term). Variations in the activity of the antioxidant enzymes, namely, catalase (CAT) and superoxide dismutase (SOD), as well as lipid peroxidation products (LPP) were measured as biomarkers of metal toxicity. Our results showed a significant (P < 0.05) increase in LPP (indicating oxidative stress) and CAT activity (indicating an adaptive response of the PL for protection against oxidative stress) in the exposed PL for all periods of exposure. However, SOD activity significantly (P < 0.05) decreased on 20 and 30 days exposure, indicating susceptibility of the PL to oxidative stress upon long-term exposure. Therefore, CAT can serve as a better biomarker of oxidative stress than SOD to long-term copper toxicity. Our results indicate that copper contamination causes oxidative stress even at sublethal doses in Penaeus indicus PL, which can thus be used as a potential biomarker of copper toxicity for long-term monitoring of coastal marine ecosystems.

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