scholarly journals Heterogeneity of penicillin-non-susceptible group B streptococci isolated from a single patient in Germany

2019 ◽  
Vol 75 (2) ◽  
pp. 296-299 ◽  
Author(s):  
Mark van der Linden ◽  
Rafael Mamede ◽  
Natascha Levina ◽  
Peter Helwig ◽  
Pedro Vila-Cerqueira ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Genomic Analysis ◽  
Amino Acid Sequences ◽  
Capsular Type ◽  
Group B Streptococci ◽  
The Us ◽  
Type Ia ◽  
The Usa ◽  
Group B ◽  
Host Evolution

Abstract Objectives Streptococcus agalactiae [group B streptococci (GBS)] have been considered uniformly susceptible to penicillin. However, increasing reports from Asia and North America are documenting penicillin-non-susceptible GBS (PRGBS) with mutations in pbp genes. Here we report, to the best of our knowledge, the first two PRGBS isolates recovered in Europe (AC-13238-1 and AC-13238-2), isolated from the same patient. Methods Two different colony morphologies of GBS were noted from a surgical abscess drainage sample. Both were serotyped and antimicrobial susceptibility testing was performed by different methodologies. High-throughput sequencing was done to compare the isolates at the genomic level, to identify their capsular type and ST, to evaluate mutations in the pbp genes and to compare the isolates with the genomes of other PRGBS isolates sharing the same serotype and ST. Results Isolates AC-13238-1 and AC-13238-2 presented MICs above the EUCAST and CLSI breakpoints for penicillin susceptibility. Both shared the capsular type Ia operon and ST23. Genomic analysis uncovered differences between the two isolates in seven genes, including altered pbp genes. Deduced amino acid sequences revealed critical substitutions in PBP2X in both isolates. Comparison with serotype Ia clonal complex 23 PRGBS from the USA reinforced the similarity between AC-13238-1 and AC-13238-2, and their divergence from the US strains. Conclusions Our results support the in-host evolution of β-lactam-resistant GBS, with two PRGBS variants being isolated from one patient.

scholarly journals Isolation, chemical composition, and molecular size of extracellular type II and type Ia polysaccharides of group B streptococci.

1983 ◽  
Vol 41 (2) ◽  
pp. 527-534 ◽  
Author(s):  
B J De Cueninck ◽  
T F Greber ◽  
T K Eisenstein ◽  
R M Swenson ◽  
G D Shockman
Keyword(s):  
Chemical Composition ◽  
Molecular Size ◽  
Type Ii ◽  
Group B Streptococci ◽  
Type Ia ◽  
Group B

Cross protection in mice with the Smith diffuse strain of Staphylococcus aureus versus a type Ia strain of group B streptococci

1982 ◽  
Vol 28 (7) ◽  
pp. 726-732 ◽  
Author(s):  
Yoshitoshi Ichiman ◽  
Kosaku Yoshida
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Surface ◽  
Active Immunization ◽  
Cell Vaccine ◽  
Group B Streptococci ◽  
Type Ia ◽  
Whole Cell Vaccine ◽  
Surface Polysaccharides ◽  
Surface Polysaccharide ◽  
Group B

Active immunization of mice with whole cell vaccine or cell surface polysaccharide from either the Smith diffuse strain of Staphylococcus aureus or SS-615 (type Ia of group B streptococci) protected against challenge by either the homologous or heterologous strains. In the peritoneal cavity of mice immunized with either of these organisms rapid phagocytosis and reduction of the viable cells was observed at 6 h after the challenge. Cell surface polysaccharides extracted from strains Smith diffuse and SS-615, both prepared by the same procedure as that of the Smith surface antigen, were capable of absorbing the protective antibody in rabbit hyperimmune sera prepared with homologous or heterologous strains.

scholarly journals Prevalence and molecular characterization of group B streptococcus in pregnant women from hospitals in Ohangwena and Oshikoto regions of Namibia

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Erastus Lafimana Haimbodi ◽  
Munyaradzi Mukesi ◽  
Sylvester Rodgers Moyo
Keyword(s):  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Group B Streptococcus ◽  
Tetracycline Resistance ◽  
Geographic Location ◽  
Molecular Characteristics ◽  
Capsular Type ◽  
Type Ia ◽  
Positive Rate ◽  
Group B

Abstract Background The main purpose of this study was to investigate the prevalence rate, antimicrobial susceptibility patterns and molecular characteristics of Streptococcus agalactiae isolated from pregnant women at 35 weeks of gestation and above, who attended antenatal screening at selected hospitals in Ohangwena and Oshikoto regions of Namibia. Results Out of 210 women screened for Group B Streptococcus (GBS), 12 (5.7%) were colonised of which 25.0% were colonised rectovaginally, 58.0% vaginally and 17.0% rectally. No significant association was reported between GBS colonisation and maternal age, geographic location, marital status, education, employment, parity, still births and miscarriages (P values > 0.05). Antimicrobial susceptibility was reported at 100% for ampicillin, penicillin & ceftriaxone which are commonly used for empiric treatment of infection with GBS. Resistance to tetracycline was reported at 100%. Tetracycline resistance gene tet(M) was present in 88.9% of the isolates only and none of the isolates presented with tet(O). Polysaccharide capsular type Ia was found in 9(50%) and Ib was found in 1(5.5%) of the total isolates. The remaining isolates were not typeable using PCR. Conclusion Streptococcus agalactiae’s positive rate was 5.7% among the pregnant women examined. Socio-demographic and obstetric factors had no influence on GBS colonisation (P values > 0.05). No resistance was reported to ampicillin, penicillin and ceftriaxone. No sensitivity was reported to tetracycline. Fifty percent of the isolates were capsular type Ia, 5.5% were type Ib and 44.4% were not typeable using PCR. The study provides crucial information for informing policy in screening of GBS in pregnant women.

scholarly journals Lethal synergism induced in mice by influenza type A virus and type Ia group B streptococci.

1983 ◽  
Vol 41 (2) ◽  
pp. 618-623 ◽  
Author(s):  
W T Jones ◽  
J H Menna ◽  
D E Wennerstrom
Keyword(s):  
Type A ◽  
Group B Streptococci ◽  
Influenza Type ◽  
Type Ia ◽  
Group B

scholarly journals TWO SEROLOGICAL TYPES OF GROUP B HEMOLYTIC STREPTOCOCCI WITH RELATED, BUT NOT IDENTICAL, TYPE-SPECIFIC SUBSTANCES

1938 ◽  
Vol 67 (1) ◽  
pp. 25-40 ◽  
Author(s):  
Rebecca C. Lancefield
Keyword(s):  
Group B Streptococci ◽  
Passive Protection ◽  
Cross Reactions ◽  
Precipitin Reaction ◽  
Specific Substance ◽  
Type Ia ◽  
Preliminary Study ◽  
The Cross ◽  
Group B ◽  
Identical Type

1. Among group B hemolytic streptococci one serological type previously described as homogeneous has been shown, instead, to contain two closely related types, distinguishable by reciprocal absorption experiments. These streptococci are designated Types Ia and Ib. 2. Homologous organisms in each case absorb all antibodies from their respective antisera, while organisms of the heterologous related type absorb only the antibody responsible for the cross reactions. Group B streptococci of other types do not absorb the antibodies responsible for the cross reactions between these two related types. The precipitin reaction and passive protection tests in mice were employed in this analysis. 3. The type-specific substance of Type Ia is a polysaccharide. Preliminary study indicates that this is also true of Type Ib. While no data are available concerning the chemical relationships of these substances, it seems probable that the two types elaborate polysaccharides, related chemically as well as serologically.

scholarly journals 895 ANTIBODY-INDEPENDENT CLASSICAL PATHWAY (CP)-MEDIATED OPSONOPHAGOCYTOSIS (OP) OF TYPE Ia GROUP B STREPTOCOCCI (IaGBS)

1981 ◽  
Vol 15 ◽  
pp. 592-592
Author(s):  
Carol J Baker ◽  
Morven S Edwards ◽  
Bette J Webb ◽  
Dennis L Kasper
Keyword(s):  
Classical Pathway ◽  
Group B Streptococci ◽  
Type Ia ◽  
Group B

scholarly journals Prevalence and Molecular Characterization of Group B Streptococcus in Pregnant Women from Hospitals in Ohangwena and Oshikoto Regions of Namibia.

2020 ◽  
Author(s):  
Sylvester Rodgers Moyo ◽  
Erastus Lafimana Haimbodi ◽  
Munyaradzi Mukesi
Keyword(s):  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Group B Streptococcus ◽  
Tetracycline Resistance ◽  
Molecular Characteristics ◽  
Capsular Type ◽  
P Values ◽  
Type Ia ◽  
Positive Rate ◽  
Group B

Abstract BackgroundThe main purpose of this study was to investigate the prevalence rate, antimicrobial susceptibility patterns and molecular characteristics of Streptococcus agalactiae isolated from pregnant women at 35 weeks of gestation and above, who attended antenatal screening at selected hospitals in Ohangwena and Oshikoto regions of Namibia.ResultsTwelve (5.7%) of women screened were colonised by GBS, of which 25.0% were colonised rectovaginally, 58.0% vaginally and 17.0% rectally. No significant association was reported between GBS colonisation and maternal age, habitat, marital status, education, employment, parity, still births and miscarriages (P values >0.05). Antimicrobial susceptibility was reported at 100% for ampicillin, penicillin & ceftriaxone. Resistance to tetracycline was reported at 100%. Tetracycline resistance gene tet(M) was present in 88.9% of the isolates only and none of the isolates presented with tet(O). Polysaccharide capsular type Ia was found in 9(50%) and Ib was found in 1(5.5%) of the total isolates. The remaining isolates were not typeable using PCR.ConclusionStreptococcus agalactiae’s positive rate was 5.7% among the pregnant women examined. Socio-demographic and obstetric factors had no influence on GBS colonisation (P values >0.05). No resistance was reported to ampicillin, penicillin and ceftriaxone. No sensitivity was reported to tetracycline. Fifty (50%) of the isolates were capsular type Ia, 5.5% were type Ib and 44.4% were not typeable using PCR.

Antibody-Independent Activation of C1 by Type Ia Group B Streptococci

1982 ◽  
Vol 146 (5) ◽  
pp. 665-672 ◽  
Author(s):  
M. E. Eads ◽  
N. J. Levy ◽  
D. L. Kasper ◽  
C. J. Baker ◽  
A. Nicholson-Weller
Keyword(s):  
Group B Streptococci ◽  
Type Ia ◽  
Group B
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