scholarly journals Improved determination of Neisseria gonorrhoeae gyrase A genotype results in clinical specimens

2019 ◽  
Author(s):  
Lao-Tzu Allan-Blitz ◽  
Olivia L Ellis ◽  
Rachel Wee ◽  
Annie Truong ◽  
Samantha M Ebeyan ◽  
...  
Keyword(s):  
High Resolution ◽  
Neisseria Gonorrhoeae ◽  
Drug Resistant ◽  
High Resolution Melt ◽  
Clinical Specimens ◽  
High Resolution Melt Analysis ◽  
Gyrase A ◽  
Rapid Molecular Assays ◽  
Sydney Australia

Abstract Background The emergence of drug-resistant Neisseria gonorrhoeae has prompted the development of rapid molecular assays designed to determine antimicrobial susceptibility. One common assay uses high-resolution melt analysis to target codon 91 of the gyrase A gene (gyrA) to predict N. gonorrhoeae susceptibility to ciprofloxacin. Methods We extracted DNA from remnant clinical specimens that had previously tested positive for N. gonorrhoeae using the Aptima Combo 2 for CT/NG assay (Hologic, San Diego, CA, USA). We selected DNA extracts from specimens with indeterminate, WT and mutant gyrA genotype results from a previous study using high-resolution melt analysis to detect the gyrA codon 91 mutation. We re-tested those specimens using the recently CE-marked ResistancePlus GC (beta) assay (SpeeDx, Sydney, Australia). Results Of 86 specimens with indeterminate gyrA genotypes on high-resolution melt analysis, the ResistancePlus GC (beta) assay (SpeeDx) identified 30 (35%) WT, 22 (26%) mutant and 34 (40%) indeterminate gyrA genotypes. Conclusions The ResistancePlus GC (beta) assay showed improved N. gonorrhoeae gyrA genotype determination compared with a prior gyrA genotypic high-resolution melt assay.

scholarly journals High-resolution melt analysis to detect sequence variations in highly homologous gene regions: application to CYP2B6

2013 ◽  
Vol 14 (8) ◽  
pp. 913-922 ◽  
Author(s):  
Greyson P Twist ◽  
Roger Gaedigk ◽  
J Steven Leeder ◽  
Andrea Gaedigk
Keyword(s):  
High Resolution ◽  
Homologous Gene ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis ◽  
Sequence Variations

A rapid method for sex identification in Cannabis sativa using High Resolution Melt (HRM) analysis.

Botany ◽  
2021 ◽  
Author(s):  
Erin Jacqueline Gilchrist ◽  
Daniela Hegebarth ◽  
Shumin Wang ◽  
Teagen D. Quilichini ◽  
Jason Sawler ◽  
...  
Keyword(s):  
High Resolution ◽  
Rapid Method ◽  
X Chromosome ◽  
Cannabis Sativa ◽  
Sex Identification ◽  
Male Plant ◽  
High Resolution Melt ◽  
Male And Female ◽  
High Resolution Melt Analysis ◽  
Plant Sex

We report the identification of two SNPs in Cannabis sativa that are associated with female and male plant sex phenotypes, and are located on the top arm of the X chromosome. High Resolution Melt analysis was used to develop and validate a novel, rapid method for sex identification in medical/recreational cannabis as well as in hemp. This method can distinguish between dioecious male (XY) and dioecious female (XX) cannabis plants with 100% accuracy, and can also be used to differentiate between male and female Humulus lupulus (hop) plants.

High-Resolution Melt Analysis

2013 ◽  
pp. 409-422 ◽  
Author(s):  
Einar Berg ◽  
Tania Nolan
Keyword(s):  
High Resolution ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis

scholarly journals Detecting and Differentiating Phytoplasmas Belonging to Subgroups 16SrIV-A and 16SrIV-D Associated With Lethal Declines of Palms in Florida Using qPCR and High-Resolution Melt Analysis (HRMA)

Plant Disease ◽  
2017 ◽  
Vol 101 (8) ◽  
pp. 1449-1454 ◽  
Author(s):  
Brian W. Bahder ◽  
Ericka E. Helmick ◽  
Nigel A. Harrison
Keyword(s):  
High Resolution ◽  
Sequence Data ◽  
Management Strategies ◽  
Economic Losses ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis ◽  
Melting Temperatures ◽  
Detection And Identification ◽  
Lethal Yellowing ◽  
Sufficient Variation

Lethal yellowing (LY) and Texas Phoenix palm decline (TPPD) are two important phytoplasma diseases of palms in Florida. Both have been responsible for major economic losses historically and remain a constant threat to the sustainability of palm production in the landscaping and nursery industries in Florida. These two diseases cause rapid, lethal decline in afflicted palms, so rapid detection and identification is crucial to implement appropriate management strategies to reduce further spread and losses. In this study, a qPCR assay was developed to detect and identify the causal agents of LY and TPPD. Based on sequence data of the 16S gene for the 16SrIV-A phytoplasma (LY) and the 16SrIV-D phytoplasma (TPPD), two regions were identified in the gene that possessed sufficient variation to yield amplicons with measurable differences in melting temperature based on high resolution melt analysis (HRMA). One region was in the 5′ region and the other was located in the 3′ region of the gene. Products from both regions yielded amplicons with significantly different melting temperatures between the two phytoplasma strains. This research allows for the detection and identification of phytoplasmas in palms rapidly by eliminating many lengthy and post-PCR steps commonly used in phytoplasma identification.

Frequency of allotype “b” in human platelet antigen 1 to 29 systems among blood donors in Japan estimated using high‐resolution melt analysis

Transfusion ◽  
2020 ◽  
Vol 60 (11) ◽  
pp. 2702-2713
Author(s):  
Tomoya Hayashi ◽  
Ryota Aminaka ◽  
Hiroyuki Ishii ◽  
Yoshihiko Tani ◽  
Yoshihiro Fujimura ◽  
...  
Keyword(s):  
High Resolution ◽  
Blood Donors ◽  
Human Platelet ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis ◽  
Platelet Antigen ◽  
Human Platelet Antigen

Real-time PCR and high-resolution melt analysis methods for detection of pathogenic species of Brucella

2017 ◽  
Vol 41 (6) ◽  
Author(s):  
Faramarz Masjedian Jazi ◽  
Reza Mirnejad ◽  
Vahhab Piranfar ◽  
Noor Amir Mozafari ◽  
Taghi Zahraei Salehi ◽  
...  
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Serum Antibody ◽  
Clinical Samples ◽  
Species Differentiation ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis ◽  
Antibody Titers ◽  
Polymerase Chain ◽  
Animal Isolates

AbstractBackground:It is of great importance to quickly and accurately detectMethods:The current study describes a new method for the detection of brucellosis in clinical samples using real-time polymerase chain reaction (PCR) and high-resolution melt (HRM) curve analysis. This study was conducted on 70 human and 55 animal isolates with more than 1/80 serum antibody titers. Additionally, the accuracy and specificity of the methods were compared.Results:The mean range [cycles threshold±standard deviation (CConclusions:The results of HRM analysis can be used for species differentiation and bacterial genotyping according to nucleotide polymorphism. This molecular method could help in diagnosing

Sign in / Sign up

Export Citation Format

Share Document