Characterization of the stability and dynamics of Tn6330 in an Escherichia coli strain by nanopore long reads

2019 ◽  
Vol 74 (7) ◽  
pp. 1807-1811 ◽  
Author(s):  
Ruichao Li ◽  
Kaichao Chen ◽  
Edward Wai-Chi Chan ◽  
Sheng Chen
Keyword(s):  
Escherichia Coli ◽  
Coli Strain ◽  
Long Reads ◽  
The Stability

scholarly journals Cloning and characterization of the eae gene from a dog attaching and effacing Escherichia coli strain 4221

2006 ◽  
Vol 148 (2) ◽  
pp. 239-245 ◽  
Author(s):  
Hongyan An ◽  
John M Fairbrother ◽  
J.Daniel Dubreuil ◽  
Josée Harel
Keyword(s):  
Escherichia Coli ◽  
Coli Strain

scholarly journals Characterization of a gne::IS629 O Rough:H7 Escherichia coli Strain from a Hemorrhagic Colitis Patient

2010 ◽  
Vol 76 (15) ◽  
pp. 5290-5291 ◽  
Author(s):  
Lydia V. Rump ◽  
Lothar Beutin ◽  
Markus Fischer ◽  
Peter C. H. Feng
Keyword(s):  
Escherichia Coli ◽  
Coli Strain ◽  
Hemorrhagic Colitis ◽  
Colitis Patient ◽  
Pathogenic Potential

ABSTRACT Shiga-toxigenic Escherichia coli strains that are O rough:H7 due to gne::IS629 were thought to be rare and to have unknown pathogenic potential. Recently, an O rough:H7 strain caused by gne::IS629 was isolated from a hemorrhagic colitis patient, suggesting that these strains are pathogenic and may not be as rare as anticipated.

scholarly journals Complete Circular Genome Sequence of a Multidrug-Resistant Escherichia coli Strain from Cuba Obtained with Nanopore and Illumina Hybrid Assembly

2019 ◽  
Vol 8 (48) ◽  
Author(s):  
Rosa Elena Hernández-Fillor ◽  
Michael Brilhante ◽  
Ivette Espinosa ◽  
Vincent Perreten
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Antibiotic Resistance Genes ◽  
Generation Cephalosporin ◽  
Multidrug Resistant ◽  
Coli Strain ◽  
Hybrid Assembly ◽  
Content Type ◽  
Long Reads ◽  
Cephalosporin Resistance

The complete genome sequence of a multidrug-resistant Escherichia coli strain isolated from a healthy pig in Cuba was determined using short and long reads. This strain carried four plasmids, including a 42,683-kb IncX1 plasmid, which contains the third-generation cephalosporin resistance gene bla CTX-M-32 together with other disinfectant and antibiotic resistance genes.

scholarly journals Isolation and Characterization of a PEG-degrading and Mo-reducing Escherichia coli strain Amr-13 in soils from Egypt

2021 ◽  
Vol 9 (2) ◽  
pp. 23-29
Author(s):  
Nubli Shuhaimi ◽  
M. Abd AbdEl-Mongy ◽  
N.A. Shamaan ◽  
Chaing Hin Lee ◽  
M.A. Syed ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Parameters ◽  
Sodium Molybdate ◽  
Gompertz Model ◽  
Coli Strain ◽  
Molybdenum Blue ◽  
Isolation And Characterization ◽  
Specific Growth Rates ◽  
Peg 600

Molybdenum is a pollutant that shows toxicity to spermatogenesis while polyethylene glycols (PEG) are used predominantly in detergents. The pollution of molybdenum and PEGs are reported worldwide. We have isolated ten molybdenum-reducing bacterial isolates from soil that can reduce molybdenum (sodium molybdate) into the colloidal molybdenum blue (Mo-blue). The screening of these isolates for PEG-degrading ability showed that one isolate was capable to utilize PEG 200, 300 and 600 for optimal conditions were pHs between 5.5 and 8.0, temperatures between 30 and 37 oC, phosphate at 5 mM, molybdate between 10 and 30 mM, and glucose as the electron donor. Biochemical analysis of the bacterium identifies it as Escherichia coli strain Amr-13. Growth was best supported by all PEGs at concentrations of between 600 and 1,000 mg/L. A complete degradation for PEG 200 and PEG 300 at 1,000 mg/L was observed on day four and five, respectively, while nearly 90% of PEG 600 was degraded on day six. The growth of this bacterium on these PEGs was modelled using the modified Gompertz model, and produced growth parameters values, which were maximum specific growth rates of 1.51, 1.45 and 1.18 d-1 and lag periods of 0.53, 0.87 and 1.02 day for PEG 200, PEG 300 and PEG 600, respectively. PEG 200 was the most preferred substrate for this bacterium, while PEG 600 was the least preferred.

scholarly journals Genomic and Proteomic Characterization of the Extended-Spectrum β-Lactamase (ESBL)-Producing Escherichia coli Strain CCUG 73778: A Virulent, Nosocomial Outbreak Strain

2020 ◽  
Vol 8 (6) ◽  
pp. 893 ◽  
Author(s):  
Daniel Jaén-Luchoro ◽  
Antonio Busquets ◽  
Roger Karlsson ◽  
Francisco Salvà-Serra ◽  
Christina Åhrén ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Coli Strain ◽  
University Hospital ◽  
Hospital Environment ◽  
Resistance Factors ◽  
E Coli ◽  
Extended Spectrum ◽  
Liquid Chromatography Tandem Mass

Escherichia coli strain CCUG 78773 is a virulent extended-spectrum β-lactamase (ESBL)-producing ST131-O25b type strain isolated during an outbreak at a regional university hospital. The complete and closed genome sequence, comprising one chromosome (5,076,638 bp) and six plasmids (1718–161,372 bp), is presented. Characterization of the genomic features detected the presence of 59 potential antibiotic resistance factors, including three prevalent β-lactamases. Several virulence associated elements were determined, mainly related with adherence, invasion, biofilm formation and antiphagocytosis. Twenty-eight putative type II toxin-antitoxin systems were found. The plasmids were characterized, through in silico analyses, confirming the two β-lactamase-encoding plasmids to be conjugative, while the remaining plasmids were mobilizable. BLAST analysis of the plasmid sequences showed high similarity with plasmids in E. coli from around the world. Expression of many of the described virulence and AMR factors was confirmed by proteomic analyses, using bottom-up, liquid chromatography-tandem mass spectrometry (LC-MS/MS). The detailed characterization of E. coli strain CCUG 78773 provides a reference for the relevance of genetic elements, as well as the characterization of antibiotic resistance and the spread of bacteria harboring ESBL genes in the hospital environment.

scholarly journals Cloning and characterization of the iron uptake gene iutA from avian Escherichia coli

2008 ◽  
Vol 51 (3) ◽  
pp. 473-482 ◽  
Author(s):  
Dorismey Vieira Tokano ◽  
Marisa Emiko Kawaichi ◽  
Emerson José Venâncio ◽  
Marilda Carlos Vidotto
Keyword(s):  
Escherichia Coli ◽  
Biological Activity ◽  
Iron Uptake ◽  
Expression Vector ◽  
Iron Acquisition ◽  
Coli Strain ◽  
High Yield ◽  
High Similarity ◽  
E Coli

The aim of this work was to isolate, clone and characterize the iron uptake gene iutA from avian pathogenic E. coli (APEC). The iutA gene was isolated from the strain APEC 9, serotype O2:H9, which was cloned in the expression vector pET101/D-TOPO. The gene of 2.2 Kb was sequenced (AY602767, which showed high similarity to the iutA gene from three plasmids, two from APEC, pAPEC-02-ColV (AY545598.4) and pTJ100 (AY553855.1), and one from a human invasive E. coli strain, the pColV K30. The recombinant protein IutA was over expressed in E. coli BL21(DE-3) and was solubilized with urea and purified by Ni-NTA column. This method produced a relatively high yield of r-IutA of approximately 74kDa, which was used to produce the antibody anti-IutA. This anti-IutA reacted with the protein r-IutA and native IutA of APEC 9, as demonstrated by Western blot, showing that the r-IutA conserved epitopes and its antigenicity was preserved. The anti-IutA IgY was able to inhibit the IutA biological activity, inhibiting the sensitivity to cloacin DF13 of APEC9. However, it did not inhibit the growth of APEC9 in M9 and did not protect the chickens inoculated with the APEC, suggesting that the APEC possessed another iron acquisition mechanism distinct of aerobactin.

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