scholarly journals European external quality assessments for identification, molecular typing and characterization of Staphylococcus aureus

2018 ◽  
Vol 73 (10) ◽  
pp. 2662-2666 ◽  
Author(s):  
Ariane Deplano ◽  
Magali Dodémont ◽  
Olivier Denis ◽  
Henrik Westh ◽  
Heidi Gumpert ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Typing ◽  
External Quality ◽  
Quality Assessments

scholarly journals Detection of enterotoxins and genotyping of Staphylococcus aureus strains isolated from Isfahan Educational Hospital, Iran

2017 ◽  
Vol 32 (3) ◽  
Author(s):  
Seyed Asghar Havaei ◽  
Nahid Rezaei ◽  
Roholla Havaei ◽  
Amirmorteza Ebrahimzadeh Namvar
Keyword(s):  
Staphylococcus Aureus ◽  
Multiplex Pcr ◽  
Molecular Typing ◽  
University Hospital ◽  
Pulse Field ◽  
Pulse Field Gel Electrophoresis ◽  
Typing Methods ◽  
Method Analysis ◽  
Sequence Types

<em>Background and aims:</em> <em>Staphylococcus aureus</em> is known as one of the most important nosocomial pathogens, which may lead to several infections. The aim of this study was determining the enterotoxins A, C, and TSST-1 and molecular characterization of <em>S.</em> <em>aureus</em> strains with PFGE and MLST typing methods. <br /><em>Materials and methods:</em> In the present study during the sixmonths sampling, fifty <em>S.</em> <em>aureus</em> strains were isolated from patients admitted to Al-Zahra university hospital. Antimicrobial susceptibility testing, Multiplex PCR for detection of enterotoxin A, C and TSST-1, pulse field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used for molecular typing. <br /><em>Results</em>: In antibiogram the highest and lowest percentage of resistance was belonged to tetracycline and rifampin respectively. Multiplex PCR indicated that 30% of the strains harbored sea and 34% harbored sec genes. However, only 4% of our collected isolates had tsst gene. In PFGE method analysis on all <em>S. aureus</em> strains, a total of 19 different patterns were identified. Nine various sequence types in 27 selected <em>S. aureus</em> isolates were identified by MLST. <br /><em>Conclusions</em>: Present study indicates a possible higher variability among our <em>S. aureus</em> strains by two different molecular typing methods; nevertheless four main common types (CT1, CT7, CT9, and CT11) with at least one toxin genes were determined.

scholarly journals Serological versus molecular typing of surface-associated immune evading polysaccharide antigens-based phenotypes of Staphylococcus aureus

2014 ◽  
Vol 63 (11) ◽  
pp. 1427-1431 ◽  
Author(s):  
Charlene B. Waryah ◽  
Jully Gogoi-Tiwari ◽  
Kelsi Wells ◽  
Paul Costantino ◽  
Hani Al-Salami ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Typing ◽  
Capsular Polysaccharide ◽  
Specific Primers ◽  
Serological Typing ◽  
Specific Antisera ◽  
Polysaccharide Antigens ◽  
Typing Methods ◽  
Identification And Characterization

The aim of this study was to compare the performance of serological versus molecular typing methods to detect capsular polysaccharide (CP) and surface-associated polysaccharide antigen 336 phenotypes of Staphylococcus aureus isolates. Molecular typing of CP types 1, 5 and 8 was carried out using PCR, whereas serological typing of CP1, 2, 5, 8 and antigen 336 was carried out by slide agglutination using specific antisera. By genotyping, 14/31 strains were CP8 positive, 12/31 strains were CP5 and the remaining 6/31 isolates were non-typable (NT). One isolate was positive for both CP5 and CP8 by PCR, but was confirmed as CP8 type serologically. Detection of CP2 and type 336 by PCR was not possible because specific primers were either not available or non-specific. Using serotyping, 14/31 strains were CP8 positive, 11/31 CP5 positive and 2/31 positive for antigen 336. The remaining four S. aureus isolates were serologically NT. However, three of four NT and two 336-positive S. aureus isolates were encapsulated as determined by light microscopy after capsular staining. This discovery was surprising and warrants further investigations on the identification and characterization of additional capsular phenotypes prevalent among S. aureus clinical isolates. It was concluded that serological typing was a better method than molecular typing for use in epidemiological investigations based upon the distribution of surface-associated polysaccharide antigens-based phenotypes.

scholarly journals Genotyping and characterization of prophage patterns in clinical isolates of Staphylococcus aureus

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Mahya Dini ◽  
Leili Shokoohizadeh ◽  
Farid Aziz Jalilian ◽  
Abbas Moradi ◽  
Mohammad Reza Arabestani
Keyword(s):  
Staphylococcus Aureus ◽  
Virulence Factors ◽  
Molecular Typing ◽  
Pcr Analysis ◽  
Potential Threat ◽  
Antibiotic Resistant ◽  
The World ◽  
Resistance Patterns ◽  
Mrsa Strains

Abstract Objective Staphylococcus aureus is considered an important pathogen with a variety of virulence factors in communities and hospitals all around the world. Prophage typing is a practical technique for categorizing this bacterium. In this study, we focused on the detection of prophage patterns in methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) strains based on their virulence factors, antimicrobial resistance patterns, and molecular typing by rep-PCR. Results Out of 126 S. aureus isolates, 45 (35.7%) were identified as MRSA. In total, 17 different prophage types were detected and 112 strains out of 126 strains contained at least one prophage. There was a statistically significant relationship between hld, hlg, eta and SGA, SGA, and SGFb, respectively. The results of the rep-PCR analysis revealed 14 different patterns among the MRSA and MSSA isolates. In conclusion, the presence of different prophage-encoded virulence factors and antibiotic-resistant genes among MRSA strains enables them to produce a broad range of diseases. Thus, diverse MRSA strains which have these prophages can be considered as a potential threat to the patient’s health in either the hospital or the community.

Phylogeny Characterization of Seb Gene Encoding Enterotoxins in Staphylococcus aureus Isolated from Raw Milk and Cheese

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Fatima N. Aziz ◽  
Laith Abdul Hassan Mohammed-Jawad
Keyword(s):  
Staphylococcus Aureus ◽  
Food Poisoning ◽  
Raw Milk ◽  
Staphylococcal Enterotoxin B ◽  
Human Pathogen ◽  
Conventional Pcr ◽  
Biochemical Tests ◽  
Specific Primers ◽  
Gene Encoding

Food poisoning due to the bacteria is a big global problem in economically and human's health. This problem refers to an illness which is due to infection or the toxin exists in nature and the food that use. Milk is considered a nutritious food because it contains proteins and vitamins. The aim of this study is to detect and phylogeny characterization of staphylococcal enterotoxin B gene (Seb). A total of 200 milk and cheese samples were screened. One hundred ten isolates of Staphylococcus aureus pre-confirmed using selective and differential media with biochemical tests. Genomic DNA was extracted from the isolates and the SEB gene detects using conventional PCR with specific primers. Three staphylococcus aureus isolates were found to be positive for Seb gene using PCR and confirmed by sequencing. Sequence homology showed variety range of identity starting from (100% to 38%). Phylogenetic tree analyses show that samples (6 and 5) are correlated with S. epidermidis. This study discovered that isolates (A6-RLQ and A5-RLQ) are significantly clustered in a group with non- human pathogen Staphylococcus agnetis.

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