scholarly journals HIV-1 genotypic resistance testing using the Vela automated next-generation sequencing platform

2018 ◽  
Vol 73 (5) ◽  
pp. 1152-1157 ◽  
Author(s):  
Stéphanie Raymond ◽  
Florence Nicot ◽  
Romain Carcenac ◽  
Caroline Lefebvre ◽  
Nicolas Jeanne ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Resistance Testing ◽  
Next Generation ◽  
Genotypic Resistance ◽  
Sequencing Platform ◽  
Next Generation Sequencing Platform ◽  
Genotypic Resistance Testing ◽  
Generation Sequencing ◽  
Hiv 1

scholarly journals Comparison of anIn VitroDiagnostic Next-Generation Sequencing Assay with Sanger Sequencing for HIV-1 Genotypic Resistance Testing

2018 ◽  
Vol 56 (6) ◽  
Author(s):  
Philip L. Tzou ◽  
Pramila Ariyaratne ◽  
Vici Varghese ◽  
Charlie Lee ◽  
Elian Rakhmanaliev ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Sanger Sequencing ◽  
Low Frequency ◽  
Antiretroviral Drugs ◽  
Resistance Testing ◽  
Next Generation ◽  
Genotypic Resistance ◽  
Genotypic Resistance Testing ◽  
Generation Sequencing ◽  
Hiv 1

ABSTRACTThe ability of next-generation sequencing (NGS) technologies to detect low frequency HIV-1 drug resistance mutations (DRMs) not detected by dideoxynucleotide Sanger sequencing has potential advantages for improved patient outcomes. We compared the performance of anin vitrodiagnostic (IVD) NGS assay, the Sentosa SQ HIV genotyping assay for HIV-1 genotypic resistance testing, with Sanger sequencing on 138 protease/reverse transcriptase (RT) and 39 integrase sequences. The NGS assay used a 5% threshold for reporting low-frequency variants. The level of complete plus partial nucleotide sequence concordance between Sanger sequencing and NGS was 99.9%. Among the 138 protease/RT sequences, a mean of 6.4 DRMs was identified by both Sanger and NGS, a mean of 0.5 DRM was detected by NGS alone, and a mean of 0.1 DRM was detected by Sanger sequencing alone. Among the 39 integrase sequences, a mean of 1.6 DRMs was detected by both Sanger sequencing and NGS and a mean of 0.15 DRM was detected by NGS alone. Compared with Sanger sequencing, NGS estimated higher levels of resistance to one or more antiretroviral drugs for 18.2% of protease/RT sequences and 5.1% of integrase sequences. There was little evidence for technical artifacts in the NGS sequences, but the G-to-A hypermutation was detected in three samples. In conclusion, the IVD NGS assay evaluated in this study was highly concordant with Sanger sequencing. At the 5% threshold for reporting minority variants, NGS appeared to attain a modestly increased sensitivity for detecting low-frequency DRMs without compromising sequence accuracy.

scholarly journals Correction: First evaluation of the Next-Generation Sequencing platform for the detection of HIV-1 drug resistance mutations in Belgium

PLoS ONE ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. e0211587
Author(s):  
Géraldine Dessilly ◽  
Léonie Goeminne ◽  
Anne-thérèse Vandenbroucke ◽  
François E. Dufrasne ◽  
Anandi Martin ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Next Generation Sequencing ◽  
Next Generation ◽  
Resistance Mutations ◽  
Sequencing Platform ◽  
Drug Resistance Mutations ◽  
Next Generation Sequencing Platform ◽  
Generation Sequencing ◽  
Hiv 1

scholarly journals First evaluation of the Next-Generation Sequencing platform for the detection of HIV-1 drug resistance mutations in Belgium

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0209561 ◽  
Author(s):  
Géraldine Dessilly ◽  
Léonie Goeminne ◽  
Anne-thérèse Vandenbroucke ◽  
Francois E. Dufrasne ◽  
Anandi Martin ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Next Generation Sequencing ◽  
Next Generation ◽  
Resistance Mutations ◽  
Sequencing Platform ◽  
Drug Resistance Mutations ◽  
Next Generation Sequencing Platform ◽  
Generation Sequencing ◽  
Hiv 1

HIV-1 drug resistance mutations detection and HIV-1 subtype G report by using next-generation sequencing platform

2020 ◽  
Vol 146 ◽  
pp. 104221
Author(s):  
Mohammad Gholami ◽  
NeginHosseini Rouzbahani ◽  
SiamakMirab Samiee ◽  
Katayoun Tayeri ◽  
Khodayar Ghorban ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Next Generation Sequencing ◽  
Next Generation ◽  
Resistance Mutations ◽  
Sequencing Platform ◽  
Drug Resistance Mutations ◽  
Next Generation Sequencing Platform ◽  
Generation Sequencing ◽  
Hiv 1

Routine drug resistance testing in HIV-1 proviral DNA, using an automated next- generation sequencing assay

2019 ◽  
Vol 121 ◽  
pp. 104207 ◽  
Author(s):  
Enagnon Kazali Alidjinou ◽  
Pauline Coulon ◽  
Christophe Hallaert ◽  
Olivier Robineau ◽  
Agnès Meybeck ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Next Generation Sequencing ◽  
Resistance Testing ◽  
Next Generation ◽  
Proviral Dna ◽  
Drug Resistance Testing ◽  
Generation Sequencing ◽  
Hiv 1
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