scholarly journals A real-time PCR assay for direct characterization of theNeisseria gonorrhoeaeGyrA 91 locus associated with ciprofloxacin susceptibility

2015 ◽  
Vol 71 (2) ◽  
pp. 353-356 ◽  
Author(s):  
Cameron Buckley ◽  
Ella Trembizki ◽  
Basil Donovan ◽  
Marcus Chen ◽  
Kevin Freeman ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay

scholarly journals Characterization of Plasmodium ovale curtisi and P. ovale wallikeri in Western Kenya Utilizing a Novel Species-specific Real-time PCR Assay

2015 ◽  
Vol 9 (1) ◽  
pp. e0003469 ◽  
Author(s):  
Robin H. Miller ◽  
Clifford O. Obuya ◽  
Elizabeth W. Wanja ◽  
Bernhards Ogutu ◽  
John Waitumbi ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Novel Species ◽  
Plasmodium Ovale ◽  
Pcr Assay ◽  
Western Kenya ◽  
Plasmodium Ovale Curtisi ◽  
Species Specific

scholarly journals Characterization of a universal screening approach for congenital CMV infection based on a highly-sensitive, quantitative, multiplex real-time PCR assay

PLoS ONE ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. e0227143
Author(s):  
Angela Nagel ◽  
Emmanouela Dimitrakopoulou ◽  
Norbert Teig ◽  
Peter Kern ◽  
Thomas Lücke ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Universal Screening ◽  
Cmv Infection ◽  
Pcr Assay ◽  
Screening Approach ◽  
Highly Sensitive ◽  
Congenital Cmv Infection ◽  
Congenital Cmv

scholarly journals A Real-Time PCR Assay for Simultaneous Detection and Differentiation of Four Common Entamoeba Species That Infect Humans

2020 ◽  
Vol 59 (1) ◽  
pp. e01986-20
Author(s):  
Ibne Karim M. Ali ◽  
Shantanu Roy
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Small Subunit ◽  
Clinical Samples ◽  
Pcr Assay ◽  
Content Type ◽  
Rdna Sequences ◽  
Appropriate Treatment

ABSTRACTThere are over 40 species within the genus Entamoeba, eight of which infect humans. Of these, four species (Entamoeba histolytica, E. dispar, E. moshkovskii, and E. bangladeshi) are morphologically indistinguishable from each other, and yet differentiation is important for appropriate treatment decisions. Here, we developed a hydrolysis probe-based tetraplex real-time PCR assay that can simultaneously detect and differentiate these four species in clinical samples. In this assay, multicopy small-subunit (SSU) ribosomal DNA (rDNA) sequences were used as targets. We determined that the tetraplex real-time PCR can detect amebic DNA corresponding to as little as a 0.1 trophozoite equivalent of any of these species. We also determined that this assay can detect E. histolytica DNA in the presence of 10-fold more DNA from another Entamoeba species in mixed-infection scenarios. With a panel of more than 100 well-characterized clinical samples diagnosed and confirmed using a previously published duplex real-time PCR (capable of detecting E. histolytica and E. dispar), our tetraplex real-time PCR assay demonstrated levels of sensitivity and specificity comparable with those demonstrated by the duplex real-time PCR assay. The advantage of our assay over the duplex assay is that it can specifically detect two additional Entamoeba species and can be used in conventional PCR format. This newly developed assay will allow further characterization of the epidemiology and pathogenicity of the four morphologically identical Entamoeba species, especially in low-resource settings.

scholarly journals Genomic Characterization of Canine Circovirus Detected in Red Foxes (Vulpes vulpes) from Italy using a New Real-Time PCR Assay

2020 ◽  
Vol 56 (1) ◽  
pp. 239 ◽  
Author(s):  
Stefano De Arcangeli ◽  
Andrea Balboni ◽  
Elisa Kaehler ◽  
Lorenza Urbani ◽  
Ranieri Verin ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Vulpes Vulpes ◽  
Pcr Assay ◽  
Red Foxes ◽  
Genomic Characterization
Sign in / Sign up

Export Citation Format

Share Document