Four novel plasmids from Staphylococcus hyicus and CoNS that carry a variant of the spectinomycin resistance gene spd

2014 ◽  
Vol 70 (3) ◽  
pp. 948-949 ◽  
Author(s):  
Sarah Wendlandt ◽  
Kristina Kadlec ◽  
Stefan Schwarz
Keyword(s):  
Resistance Gene ◽  
Spectinomycin Resistance

The ancient small mobilizable plasmid pALWED1.8 harboring a new variant of the non-cassette streptomycin/spectinomycin resistance gene aadA27

Plasmid ◽  
2016 ◽  
Vol 84-85 ◽  
pp. 36-43 ◽  
Author(s):  
Anton Kurakov ◽  
Sofia Mindlin ◽  
Alexey Beletsky ◽  
Natalya Shcherbatova ◽  
Andrey Rakitin ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Spectinomycin Resistance ◽  
Mobilizable Plasmid ◽  
New Variant

scholarly journals New Gene Cassettes for Trimethoprim Resistance,dfr13, and Streptomycin-Spectinomycin Resistance,aadA4, Inserted on a Class 1 Integron

2000 ◽  
Vol 44 (2) ◽  
pp. 355-361 ◽  
Author(s):  
Peter V. Adrian ◽  
Christopher J. Thomson ◽  
Keith P. Klugman ◽  
Sebastian G. B. Amyes
Keyword(s):  
Resistance Gene ◽  
Dihydrofolate Reductase ◽  
Antibiotic Resistance Genes ◽  
Amino Acid Identity ◽  
Aminoglycoside Resistance ◽  
Class 1 Integron ◽  
Spectinomycin Resistance ◽  
Class 1 ◽  
Dihydrofolate Reductases ◽  
A Site

ABSTRACT In a previous survey of 357 trimethoprim-resistant isolates of aerobic gram-negative bacteria from commensal fecal flora, hybridization experiments showed that 25% (90 of 357) of the isolates failed to hybridize to specific oligonucleotide probes for dihydrofolate reductase types 1, 2b, 3, 5, 6, 7, 8, 9, 10, and 12. Subsequent cloning and sequencing of a plasmid-borne trimethoprim resistance gene from one of these isolates revealed a new dihydrofolate reductase gene, dfr13, which occurred as a cassette integrated in a site-specific manner in a class 1 integron. The gene product shared 84% amino acid identity with dfr12 and exhibited a trimethoprim inhibition profile similar to that ofdfr12. Gene probing experiments with an oligonucleotide probe specific for this gene showed that 12.3% (44 of 357) of the isolates which did not hybridize to probes for other dihydrofolate reductases hybridized to this probe. Immediately downstream ofdfr13, a new cassette, an aminoglycoside resistance gene of the class AADA [ANT(3")(9)-I], which encodes streptomycin-spectinomycin resistance, was identified. This gene shares 57% identity with the consensus aadA1(ant(3")-Ia) and has been called aadA4(ant(3")-Id). The 3′ end of the aadA4 cassette was truncated by IS26, which was contiguous with a truncated form of Tn3. On the same plasmid, pUK2381, a second copy of IS26 was associated with sul2, which suggests that both integrase and transposase activities have played major roles in the arrangement and dissemination of antibiotic resistance genesdfr13, aadA4, bla TEM-1, and sul2.

scholarly journals Versatile nourseothricin and streptomycin/spectinomycin resistance gene cassettes and their use in chromosome integration vectors

2016 ◽  
Vol 129 ◽  
pp. 8-13
Author(s):  
Stephanie S. Lehman ◽  
Katherine M. Mladinich ◽  
Angkana Boonyakanog ◽  
Takehiko Mima ◽  
RoxAnn R. Karkhoff-Schweizer ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Spectinomycin Resistance ◽  
Gene Cassettes

The aminoglycoside-resistance operon of the plasmid pSa: nucleotide sequence of the streptomycin-spectinomycin resistance gene

Gene ◽  
1985 ◽  
Vol 36 (1-2) ◽  
pp. 97-104 ◽  
Author(s):  
Robert C. Tait ◽  
Hans Rempel ◽  
Raymond L. Rodriguez ◽  
Clarence I. Kado
Keyword(s):  
Nucleotide Sequence ◽  
Resistance Gene ◽  
Aminoglycoside Resistance ◽  
Spectinomycin Resistance

scholarly journals Novel Streptomycin and Spectinomycin Resistance Gene as a Gene Cassette within a Class 1 Integron Isolated fromEscherichia coli

1999 ◽  
Vol 43 (12) ◽  
pp. 3036-3038 ◽  
Author(s):  
Dorthe Sandvang
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Bacterial Species ◽  
Gene Cassette ◽  
Class 1 Integron ◽  
Gram Negative ◽  
Spectinomycin Resistance ◽  
Class 1 ◽  
Genes Encoding ◽  
New Gene

ABSTRACT The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. The present study has revealed the sequence of a new gene, aadA5, integrated as a gene cassette together with the trimethoprim resistance genedfr7 in a class 1 integron. The integron was located on a plasmid and was identified in a pathogenic porcineEscherichia coli isolate.

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