scholarly journals A sensitive and reproducible in vivo imaging mouse model for evaluation of drugs against late-stage human African trypanosomiasis

2014 ◽  
Vol 70 (2) ◽  
pp. 510-517 ◽  
Author(s):  
H. Burrell-Saward ◽  
J. Rodgers ◽  
B. Bradley ◽  
S. L. Croft ◽  
T. H. Ward
Keyword(s):  
Mouse Model ◽  
Late Stage ◽  
In Vivo Imaging ◽  
Human African Trypanosomiasis ◽  
African Trypanosomiasis

scholarly journals Cross-Resistance to Nitro Drugs and Implications for Treatment of Human African Trypanosomiasis

2010 ◽  
Vol 54 (7) ◽  
pp. 2893-2900 ◽  
Author(s):  
Antoaneta Y. Sokolova ◽  
Susan Wyllie ◽  
Stephen Patterson ◽  
Sandra L. Oza ◽  
Kevin D. Read ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Human African Trypanosomiasis ◽  
Parent Drug ◽  
Cross Resistance ◽  
Pharmacokinetic Studies ◽  
African Trypanosomiasis ◽  
Trypanosoma Brucei Brucei ◽  
Resistant Lines

ABSTRACT The success of nifurtimox-eflornithine combination therapy (NECT) for the treatment of human African trypanosomiasis (HAT) has renewed interest in the potential of nitro drugs as chemotherapeutics. In order to study the implications of the more widespread use of nitro drugs against these parasites, we examined the in vivo and in vitro resistance potentials of nifurtimox and fexinidazole and its metabolites. Following selection in vitro by exposure to increasing concentrations of nifurtimox, Trypanosoma brucei brucei nifurtimox-resistant clones designated NfxR1 and NfxR2 were generated. Both cell lines were found to be 8-fold less sensitive to nifurtimox than parental cells and demonstrated cross-resistance to a number of other nitro drugs, most notably the clinical trial candidate fexinidazole (∼27-fold more resistant than parental cells). Studies of mice confirmed that the generation of nifurtimox resistance in these parasites did not compromise virulence, and NfxR1 remained resistant to both nifurtimox and fexinidazole in vivo. In the case of fexinidazole, drug metabolism and pharmacokinetic studies indicate that the parent drug is rapidly metabolized to the sulfoxide and sulfone form of this compound. These metabolites retained trypanocidal activity but were less effective in nifurtimox-resistant lines. Significantly, trypanosomes selected for resistance to fexinidazole were 10-fold more resistant to nifurtimox than parental cells. This reciprocal cross-resistance has important implications for the therapeutic use of nifurtimox in a clinical setting and highlights a potential danger in the use of fexinidazole as a monotherapy.

scholarly journals Multimodal In Vivo Imaging of Tumorigenesis and Response to Chemotherapy in a Transgenic Mouse Model of Mammary Cancer

2015 ◽  
Vol 18 (4) ◽  
pp. 617-626 ◽  
Author(s):  
Jean-Louis Alberini ◽  
Raphaël Boisgard ◽  
Stéphanie Guillermet ◽  
Karine Siquier ◽  
Benoît Jego ◽  
...  
Keyword(s):  
Mouse Model ◽  
Transgenic Mouse ◽  
In Vivo Imaging ◽  
Mammary Cancer ◽  
Transgenic Mouse Model ◽  
Response To Chemotherapy

scholarly journals Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Rhiannon P. Murrie ◽  
Freda Werdiger ◽  
Martin Donnelley ◽  
Yu-wei Lin ◽  
Richard P. Carnibella ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Lung Function ◽  
Mouse Model ◽  
Real Time ◽  
In Vivo Imaging ◽  
X Ray ◽  
Regional Lung Function ◽  
Regional Lung

scholarly journals In vivo imaging of activated microglia in a mouse model of focal cerebral ischemia by two-photon microscopy

2015 ◽  
Vol 6 (9) ◽  
pp. 3303 ◽  
Author(s):  
Seoyeon Bok ◽  
Taejun Wang ◽  
Chan-Ju Lee ◽  
Seong-Uk Jeon ◽  
Young-Eun Kim ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Mouse Model ◽  
In Vivo Imaging ◽  
Focal Cerebral Ischemia ◽  
Activated Microglia ◽  
Two Photon Microscopy ◽  
Two Photon

In vivo imaging reveals rapid morphological reactions of astrocytes towards focal lesions in an ALS mouse model

2011 ◽  
Vol 497 (2) ◽  
pp. 148-151 ◽  
Author(s):  
Payam Dibaj ◽  
Heinz Steffens ◽  
Jana Zschüntzsch ◽  
Frank Kirchhoff ◽  
Eike D. Schomburg ◽  
...  
Keyword(s):  
Mouse Model ◽  
In Vivo Imaging ◽  
Focal Lesions

In Vivo Imaging of Lymph Node Migration of MNP- and 111In-Labeled Dendritic Cells in a Transgenic Mouse Model of Breast Cancer (MMTV-Ras)

2011 ◽  
Vol 14 (2) ◽  
pp. 183-196 ◽  
Author(s):  
Cristina Martelli ◽  
Manuela Borelli ◽  
Luisa Ottobrini ◽  
Veronica Rainone ◽  
Anna Degrassi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dendritic Cells ◽  
Lymph Node ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
In Vivo Imaging ◽  
Transgenic Mouse Model

scholarly journals In Vitro and In Vivo Activities of 2-Aminopyrazines and 2-Aminopyridines in Experimental Models of Human African Trypanosomiasis

2012 ◽  
Vol 57 (2) ◽  
pp. 1012-1018 ◽  
Author(s):  
S. K. Vodnala ◽  
T. Lundback ◽  
B. Sjoberg ◽  
R. Svensson ◽  
M. E. Rottenberg ◽  
...  
Keyword(s):  
Human African Trypanosomiasis ◽  
Experimental Models ◽  
African Trypanosomiasis
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