scholarly journals Detection of mecC methicillin-resistant Staphylococcus aureus with a semi-selective enrichment broth

2014 ◽  
Vol 69 (10) ◽  
pp. 2864-2864 ◽  
Author(s):  
C. Ekenberg ◽  
K. Boye ◽  
K. Schonning ◽  
H. Westh ◽  
G. Lisby
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Enrichment Broth ◽  
Methicillin Resistant ◽  
Selective Enrichment ◽  
Selective Enrichment Broth

scholarly journals Evaluation of a Rapid Culture-Based Screening Test for Detection of Methicillin Resistant Staphylococcus aureus

2011 ◽  
Vol 60 (3) ◽  
pp. 265-268
Author(s):  
BOUSHRA FWITY ◽  
RALF LOBMANN ◽  
ANDREAS AMBROSCH
Keyword(s):  
Staphylococcus Aureus ◽  
Rapid Detection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Detection Method ◽  
Latex Agglutination ◽  
Enrichment Broth ◽  
Methicillin Resistant ◽  
Selective Enrichment ◽  
Vitek 2 ◽  
Selective Enrichment Broth

The performance of a culture based assay, BacLite Rapid MRSA for the rapid detection (5 hours) of methicillin resistant Staphylococcus aureus (MRSA) from specimens (n = 377) obtained from nares, throat, wounds and perineum was investigated. Compared to culture based reference methods (chromogenic MRSA ID (bioMerieux)), selective enrichment broth, PBP2' latex agglutination (Oxoid) and VITEK 2 identification (bioMerieux), an overall sensitivity of 71% with a 82% specificity and a negative predictive value (NPV) of 95% was provided. The Baclite test is rapid and easy to use and has the advantage of a culture-based detection method for MRSA.

A multipathogen selective enrichment broth for simultaneous growth ofSalmonella entericaserovar Enteritidis,Staphylococcus aureus, andListeria monocytogenes

2010 ◽  
Vol 56 (7) ◽  
pp. 585-597 ◽  
Author(s):  
Yi-Gang Yu ◽  
Hui Wu ◽  
Yuan-Yuan Liu ◽  
Su-Long Li ◽  
Xiao-Quan Yang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Individual Growth ◽  
Potassium Tellurite ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Growth Trial ◽  
Selective Enrichment Broth ◽  
Simultaneous Growth

A selective enrichment broth (SSL) was formulated to allow concurrent growth of 3 prominent food-borne pathogens: Salmonella enterica serovar Enteritidis, Staphylococcus aureus , and Listeria monocytogenes . Nalidixic acid, lithium chloride, and potassium tellurite were added as the selective agents, while sodium pyruvate and mannitol were employed as the supplemented elements. In the individual growth trial, the target pathogens were capable of growing in SSL to as high as 7–8 log10colony-forming units (CFU)/mL after 24 h incubation at 37 °C when being inoculated at 50–100 CFU/mL. In the simultaneous growth trial, the 3 combined target pathogens showed similar growth rates. The results show that SSL could support the successful simultaneous enrichment of 3 pathogens; however, SSL inhibited the growth of nontarget bacteria. In the artificial contaminated raw beef and ready-to-eat chicken, a high recovery of these 3 target pathogens was obtained in SSL. Finally, Salmonella Enteritidis, Staphylococcus aureus, and L. monocytogenes were detected from 710 suspicious food samples by SSL with real-time PCR, and no false-positive or -negative results were reported. In summary, SSL has been shown to be a suitable broth for the simultaneous detection of the 3 prominent food-borne pathogens by multipathogen detection on a single-assay platform.

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