scholarly journals Comment on: Characterization of the embB gene in Mycobacterium tuberculosis isolates from Barcelona and rapid detection of main mutations related to ethambutol resistance using a low-density DNA array

2014 ◽  
Vol 69 (8) ◽  
pp. 2298-2299 ◽  
Author(s):  
C. U. Koser ◽  
J. M. Bryant ◽  
I. Comas ◽  
S. Feuerriegel ◽  
S. Niemann ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rapid Detection ◽  
Low Density ◽  
Dna Array ◽  
Embb Gene

Detection of streptomycin and quinolone resistance in Mycobacterium tuberculosis by a low-density DNA array

Tuberculosis ◽  
2013 ◽  
Vol 93 (5) ◽  
pp. 508-514 ◽  
Author(s):  
Raquel Moure ◽  
Griselda Tudó ◽  
Rebeca Medina ◽  
Eva Vicente ◽  
José María Caldito ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Quinolone Resistance ◽  
Low Density ◽  
Dna Array

scholarly journals Molecular Characterization of Isoniazid-Resistant Mycobacterium tuberculosis Isolates Collected in Australia

2005 ◽  
Vol 49 (10) ◽  
pp. 4068-4074 ◽  
Author(s):  
Caroline Lavender ◽  
Maria Globan ◽  
Aina Sievers ◽  
Helen Billman-Jacobe ◽  
Janet Fyfe
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rapid Detection ◽  
Molecular Basis ◽  
Regulatory Region ◽  
Genotypic Diversity ◽  
Resistance Mechanisms ◽  
Wild Type ◽  
Genotypic Group ◽  
Resistant Strains

ABSTRACT Elucidation of the molecular basis of isoniazid (INH) resistance in Mycobacterium tuberculosis has led to the development of different genotypic approaches for the rapid detection of INH resistance in clinical isolates. Mutations in katG, in particular the S315T substitution, are responsible for INH resistance in a large proportion of tuberculosis cases. However, the frequency of the katG S315T substitution varies with population samples. In this study, 52 epidemiologically unrelated clinical INH-resistant M. tuberculosis isolates collected in Australia were screened for mutations at katG codon 315 and the fabG1-inhA regulatory region. Importantly, 52 INH-sensitive isolates, selected to reflect the geographic and genotypic diversity of the isolates, were also included for comparison. The katG S315T substitution and fabG1-inhA −15 C-to-T mutation were identified in 34 and 13 of the 52 INH-resistant isolates, respectively, and none of the INH-sensitive isolates. Three novel katG mutations, D117A, M257I, and G491C, were identified in three INH-resistant strains with a wild-type katG codon 315, fabG1-inhA regulatory region, and inhA structural gene. When analyzed for possible associations between resistance mechanisms, resistance phenotype, and genotypic groups, it was found that neither the katG S315T nor fabG1-inhA −15 C-to-T mutation clustered with any one genotypic group, but that the −15 C-to-T substitution was associated with isolates with intermediate INH resistance and isolates coresistant to ethionamide. In total, 90.4% of unrelated INH-resistant isolates could be identified by analysis of just two loci: katG315 and the fabG1-inhA regulatory region.

Characterization of machined polystyrene foam

1989 ◽  
Vol 47 ◽  
pp. 372-373
Author(s):  
C. W. Price ◽  
E. F. Lindsey ◽  
R. M. Franks ◽  
M. A. Lane
Keyword(s):  
Surface Finish ◽  
Cell Walls ◽  
Surface Structures ◽  
Efficient Technique ◽  
Low Density ◽  
Polystyrene Foam ◽  
Planar Surfaces ◽  
Machining Characteristics

Diamond-point turning is an efficient technique for machining low-density polystyrene foam, and the surface finish can be substantially improved by grinding. However, both diamond-point turning and grinding tend to tear and fracture cell walls and leave asperities formed by agglomerations of fragmented cell walls. Vibratoming is proving to be an excellent technique to form planar surfaces in polystyrene, and the machining characteristics of vibratoming and diamond-point turning are compared.Our work has demonstrated that proper evaluation of surface structures in low density polystyrene foam requires stereoscopic examinations; tilts of + and − 3 1/2 degrees were used for the stereo pairs. Coating does not seriously distort low-density polystyrene foam. Therefore, the specimens were gold-palladium coated and examined in a Hitachi S-800 FESEM at 5 kV.

Characterization of Low Density Glass Filled Epoxies

2003 ◽  
Author(s):  
Matthew J. Quesenberry ◽  
Phillip H. Madison ◽  
Robert E. Jensen
Keyword(s):  
Low Density
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