scholarly journals Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis

2013 ◽  
Vol 69 (2) ◽  
pp. 441-444 ◽  
Author(s):  
A. Martin ◽  
B. Imperiale ◽  
P. Ravolonandriana ◽  
A. Y. Coban ◽  
A. Akgunes ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Rapid Detection ◽  
Drug Resistant ◽  
Drug Resistant Tuberculosis ◽  
Resistant Tuberculosis ◽  
Extensively Drug Resistant ◽  
Nitrate Reductase Assay

scholarly journals Evaluation of Nitrate Reductase Assay for Rapid Detection of Drug Resistant Tuberculosis

2012 ◽  
Vol 02 (04) ◽  
pp. 138-141
Author(s):  
Ranjit Kumar Sah ◽  
Dwij Raj Bhatta ◽  
Gokarna Raj Ghimire ◽  
Jeevan Bahadur Sherchand
Keyword(s):  
Nitrate Reductase ◽  
Rapid Detection ◽  
Drug Resistant ◽  
Drug Resistant Tuberculosis ◽  
Resistant Tuberculosis ◽  
Nitrate Reductase Assay

scholarly journals Evaluation of Nitrate Reductase Assay (NRA) for rapid detection of druc resistant tuberculosis at National Tuberculosis Centre, Nepal

1970 ◽  
Vol 7 (1) ◽  
pp. 26-30
Author(s):  
PK Mandal ◽  
S Basnyat ◽  
DK Khadka ◽  
DR Bhatta
Keyword(s):  
Drug Resistance ◽  
Nitrate Reductase ◽  
Sensitivity And Specificity ◽  
Drug Susceptibility ◽  
Drug Resistant ◽  
Drug Resistant Tuberculosis ◽  
Resistant Tuberculosis ◽  
National Tuberculosis ◽  
Nitrate Reductase Assay ◽  
Hiv Aids

Background: Treatment of drug-resistant tuberculosis is often based on drug susceptibility testing results. Thus simple, rapid and economic test is very important for diagnosis of drug resistant tuberculosis and such method aids in TB control effectively. One such method is a Nitrate Reductase Assay (NRA). Objective: To evaluate feasibility and performance of Nitrate Reductase Assay in the screening of drug-resistant tuberculosis. Setting: National Tuberculosis Centre and SAARC TB and HIV/AIDS Centre, Thimi, Bhaktapur, Nepal from April 2008 to March 2009. Methods: A prospective study comparing the sensitivity and specificity of the Nitrate Reductase Assay with the gold standard Lowenstein Jensen proportion method in determining drug susceptibility pattern to four primary anti-tubercular drugs i.e. isoniazid, rifampicin, streptomycin and ethambutal among clinical isolates.Results: Among 121 specimens , the sensitivity and specificity of the Nitrate Reductase Assay for detection of Isoniazid resistance was 100% and 91%, for rifampicin was 100% and 98.95%, for streptomycin was 96% and 91.66% and for ethambutal was 100% and 98% respectively.Conclusions: The Nitrate Reductase Assay is sensitive and specific enough for the detection of drug resistant tuberculosis. It is rapid, easy to use and inexpensive, making it suitable for developing countries. Its usefulness for national drug resistance surveys should be assessed. Keywords: diagnosis; drug resistance; sensitivity; specificity; NRADOI: 10.3126/saarctb.v7i1.3960SAARC J. TUBER. LUNG DIS. HIV/AIDS 2010 VII(1) 26-30

scholarly journals Evaluation of Nitrate Reductase Assay For Rapid Detection of Drug Resistant Tuberculosis

2019 ◽  
Vol 3 (1) ◽  
pp. 44-46
Author(s):  
Ranjit Kumar Sah ◽  
Dwij Raj Bhatta ◽  
Gokarna Raj Ghimire ◽  
Bhuwaneswor Prasad Kandel ◽  
Bishnu Raj Tiwari ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Sensitivity And Specificity ◽  
Susceptibility Testing ◽  
Reference Laboratory ◽  
Drug Resistant ◽  
Drug Resistant Tuberculosis ◽  
Resistant Tuberculosis ◽  
Nitrate Reductase Assay ◽  
Mdr Tb ◽  
Proportion Method

Emergence of multidrug-resistant tuberculosis (MDR-TB) urgently demands for simple, rapid and inexpensive methods of its detection for the effective treatment of drug resistant tuberculosis, particularly in low-income countries. This prospective study was carried out at National Tuberculosis Reference Laboratory and South Asian Association of Regional Cooperation (SAARC) Tuberculosis and HIV/AIDS Centre, Thimi, Bhaktapur, Nepal, from November 2009 to May 2010 to evaluate nitrate reductase assay (NRA) efficacy for rapid determination of streptomycin, isoniazid, rifampicin and ethambutol susceptibility of Mycobacterium tuberculosis strains. A total of 113 clinical isolates of M. tuberculosis were tested for four first line antitubercular drugs by nitrate reductase assay and were compared with standard proportion method. Results were available in 7-14 days by NRA as compared to proportion method which generally takes 4-6 weeks. The sensitivity and specificity of NRA were 98.1% and 100% for isoniazid, 95.1% and 98.6% for rifampicin, 91.4% and 94.9% for streptomycin, and 78.6% and 97.9% for ethambutol respectively. Agreement between NRA and proportion method were 99.1%, 97.3%, 93.8%, 95.6% for isoniazid, rifampicin, streptomycin and ethambutol, respectively. NRA is easier, inexpensive and reliable method for susceptibility testing of Mycobacterum tuberculosis for isoniazid and rifampicin, the two most important drugs for the treatment of tuberculosis. The reduction in susceptibility testing time, and higher sensitivity and specificity of NRA method is of fundamental importance in detecting MDR-TB. Key words: Drug susceptibility, MDR-TB, NRA, proportion method

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