scholarly journals Detection of the plasmid-encoded fosfomycin resistance gene fosA3 in Escherichia coli of food-animal origin

2012 ◽  
Vol 68 (4) ◽  
pp. 766-770 ◽  
Author(s):  
J. Hou ◽  
X. Yang ◽  
Z. Zeng ◽  
L. Lv ◽  
T. Yang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Animal Origin ◽  
Food Animal ◽  
Fosfomycin Resistance

scholarly journals Lytic and Lysogenic Infection of DiverseEscherichia coli and Shigella Strains with a Verocytotoxigenic Bacteriophage

2001 ◽  
Vol 67 (9) ◽  
pp. 4335-4337 ◽  
Author(s):  
Chloe E. James ◽  
Karen N. Stanley ◽  
Heather E. Allison ◽  
Harry J. Flint ◽  
Colin S. Stewart ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Plaque Assay ◽  
Kanamycin Resistance ◽  
Lytic Infection ◽  
Lytic Cycle ◽  
Animal Origin ◽  
Kanamycin Resistance Gene ◽  
E Coli ◽  
Infected Cells

ABSTRACT A verocytotoxigenic bacteriophage isolated from a strain of enterohemorrhagic Escherichia coli O157, into which a kanamycin resistance gene (aph3) had been inserted to inactivate the verocytotoxin gene (vt2 ), was used to infect Enterobacteriaceae strains. A number ofShigella and E. coli strains were susceptible to lysogenic infection, and a smooth E. coli isolate (O107) was also susceptible to lytic infection. The lysogenized strains included different smooth E. coli serotypes of both human and animal origin, indicating that this bacteriophage has a substantial capacity to disseminate verocytotoxin genes. A novel indirect plaque assay utilizing an E. coli recA441 mutant in which phage-infected cells can enter only the lytic cycle, enabling detection of all infective phage, was developed.

scholarly journals Coexistence of SFO-1 and NDM-1 β-lactamase genes and fosfomycin resistance gene fosA3 in an Escherichia coli clinical isolate

2015 ◽  
Vol 362 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Jing-yi Zhao ◽  
Yuan-qi Zhu ◽  
Yan-nian Li ◽  
Xiao-dong Mu ◽  
Li-ping You ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Clinical Isolate ◽  
Fosfomycin Resistance

scholarly journals Identification of the Multi-Resistance Gene cfr in Escherichia coli Isolates of Animal Origin

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e102378 ◽  
Author(s):  
Hui Deng ◽  
Jian Sun ◽  
Jun Ma ◽  
Liang Li ◽  
Liang-Xing Fang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Animal Origin

Prevalence of Plasmid-Mediated Fosfomycin Resistance Gene fosA3 Among CTX-M-Producing Escherichia coli Isolates from Chickens in China

2017 ◽  
Vol 14 (4) ◽  
pp. 210-218 ◽  
Author(s):  
Wei Jiang ◽  
Shuai Men ◽  
Linghan Kong ◽  
Suzhen Ma ◽  
Yongqiang Yang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Fosfomycin Resistance

scholarly journals Escherichia coli ST131-H22 as a Foodborne Uropathogen

mBio ◽  
2018 ◽  
Vol 9 (4) ◽  
Author(s):  
Cindy M. Liu ◽  
Marc Stegger ◽  
Maliha Aziz ◽  
Timothy J. Johnson ◽  
Kara Waits ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Resolution ◽  
Human Exposure ◽  
The United States ◽  
Animal Origin ◽  
Content Type ◽  
E Coli ◽  
Food Animal ◽  
Food Animals ◽  
Human Infections

ABSTRACT Escherichia coli sequence type 131 (ST131) has emerged rapidly to become the most prevalent extraintestinal pathogenic E. coli clones in circulation today. Previous investigations appeared to exonerate retail meat as a source of human exposure to ST131; however, these studies focused mainly on extensively multidrug-resistant ST131 strains, which typically carry allele 30 of the fimH type 1 fimbrial adhesin gene (ST131-H30). To estimate the frequency of extraintestinal human infections arising from foodborne ST131 strains without bias toward particular sublineages or phenotypes, we conducted a 1-year prospective study of E. coli from meat products and clinical cultures in Flagstaff, Arizona. We characterized all isolates by multilocus sequence typing, fimH typing, and core genome phylogenetic analyses, and we screened isolates for avian-associated ColV plasmids as an indication of poultry adaptation. E. coli was isolated from 79.8% of the 2,452 meat samples and 72.4% of the 1,735 culture-positive clinical samples. Twenty-seven meat isolates were ST131 and belonged almost exclusively (n = 25) to the ST131-H22 lineage. All but 1 of the 25 H22 meat isolates were from poultry products, and all but 2 carried poultry-associated ColV plasmids. Of the 1,188 contemporaneous human clinical E. coli isolates, 24 were ST131-H22, one-quarter of which occurred in the same high-resolution phylogenetic clades as the ST131-H22 meat isolates and carried ColV plasmids. Molecular clock analysis of an international ST131-H22 genome collection suggested that ColV plasmids have been acquired at least six times since the 1940s and that poultry-to-human transmission is not limited to the United States. IMPORTANCE E. coli ST131 is an important extraintestinal pathogen that can colonize the gastrointestinal tracts of humans and food animals. Here, we combined detection of accessory traits associated with avian adaptation (ColV plasmids) with high-resolution phylogenetics to quantify the portion of human infections caused by ST131 strains of food animal origin. Our results suggest that one ST131 sublineage—ST131-H22—has become established in poultry populations around the world and that meat may serve as a vehicle for human exposure and infection. ST131-H22 is just one of many E. coli lineages that may be transmitted from food animals to humans. Additional studies that combine detection of host-associated accessory elements with phylogenetics may allow us to quantify the total fraction of human extraintestinal infections attributable to food animal E. coli strains.

scholarly journals Identification of the novel tigecycline resistance gene tet(X6) and its variants in Myroides, Acinetobacter and Proteus of food animal origin

2020 ◽  
Vol 75 (6) ◽  
pp. 1428-1431 ◽  
Author(s):  
Dejun Liu ◽  
Weishuai Zhai ◽  
Huangwei Song ◽  
Yulin Fu ◽  
Stefan Schwarz ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Binding Capacity ◽  
Homology Modelling ◽  
Bacterial Species ◽  
Animal Origin ◽  
The Novel ◽  
Food Animal ◽  
Genomic Environment ◽  
Pig Farm ◽  
Acinetobacter Spp

Abstract Objectives To report a novel tigecycline resistance gene, tet(X6), and its variants in four bacterial species isolated from chickens and pigs in China. Methods WGS was conducted to identify the suspected resistance genes in the tigecycline-resistant Myroides phaeus 18QD1AZ29W. Functional cloning, homology modelling and molecular docking were performed to compare the function with other Tet(X) variants. Retrospective screening for tet(X6) was conducted for 80 isolates in our WGS data collection, and all genomic environments of tet(X6)-positive isolates were analysed. Results The tigecycline-resistant M. phaeus 18QD1AZ29W isolated from a pig farm in Shandong in 2018 was positive for tet(X2) and a novel tet(X) gene, designated tet(X6). Tet(X6) could increase the MICs of all tested tetracyclines/glycylcyclines for Escherichia coli only 2- to 4-fold, which was possibly due to a lower tetracycline binding capacity of Tet(X6) compared with that of other Tet(X) variants. Retrospective screening showed that seven other isolates (7/80, 8.8%), comprising four Proteus spp. and three Acinetobacter spp. from chickens and pigs in Shandong and Guangdong, were positive for three different variants of tet(X6). The analysis of the genomic environment revealed that two tet(X6)-positive isolates from M. phaeus and Proteus cibarius, respectively, contained ISCR2, which may play a role in tet(X6) transmission. Conclusions This study identified a novel type of tigecycline resistance gene, tet(X6), in Myroides, Acinetobacter and Proteus from chickens and swine. Tet(X6) conferred lower tetracycline/glycylcycline MICs than other Tet(X) variants, and ISCR2 may play a role in the transmission of tet(X6).

scholarly journals Identification of fosA10, a Novel Plasmid-Mediated Fosfomycin Resistance Gene of Klebsiella pneumoniae Origin, in Escherichia coli

2020 ◽  
Vol Volume 13 ◽  
pp. 1273-1279 ◽  
Author(s):  
Ying Huang ◽  
Qingqing Lin ◽  
Qiaoli Zhou ◽  
Luchao Lv ◽  
Miao Wan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Resistance Gene ◽  
Fosfomycin Resistance

scholarly journals Plasmids of Diverse Inc Groups Disseminate the Fosfomycin Resistance Gene fosA3 among Escherichia coli Isolates from Pigs, Chickens, and Dairy Cows in Northeast China

2017 ◽  
Vol 61 (9) ◽  
Author(s):  
Xiu-Mei Wang ◽  
Zhimin Dong ◽  
Stefan Schwarz ◽  
Yao Zhu ◽  
Xin Hua ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dairy Cows ◽  
Resistance Gene ◽  
Northeast China ◽  
Sequence Type ◽  
Content Type ◽  
E Coli ◽  
Conjugative Plasmids ◽  
Fosfomycin Resistance

ABSTRACT Thirty-nine fosfomycin-resistant Escherichia coli isolates carrying fosA3 were obtained from pigs, chickens, dairy cows, and staff in four northeastern provinces of China between June 2015 and April 2016. The fosA3 gene was colocated with bla CTX-M genes on conjugative plasmids of the incompatibility groups IncN (n = 12), IncN-F33:A−:B−(n = 2), IncF33:A−:B−(n = 14), IncF14:A−:B−(n = 2), and IncI1/sequence type 136 (ST136) (n = 9). Four different genetic contexts of fosA3 were detected among the 39 E. coli isolates. Three potential epidemic plasmids circulated among E. coli strains from this region.

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