scholarly journals Molecular characterization and phylogenetic analysis of quinolone resistance-determining regions (QRDRs) of gyrA, gyrB, parC and parE gene loci in viridans group streptococci isolated from adult patients with cystic fibrosis

2010 ◽  
Vol 66 (3) ◽  
pp. 476-486 ◽  
Author(s):  
Y. Maeda ◽  
M. Murayama ◽  
C. E. Goldsmith ◽  
W. A. Coulter ◽  
C. Mason ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Adult Patients ◽  
Quinolone Resistance ◽  
Viridans Group Streptococci

scholarly journals Molecular Characterization of Fluoroquinolone-Resistant Aeromonas spp. Isolated from Imported Shrimp

2012 ◽  
Vol 78 (22) ◽  
pp. 8137-8141 ◽  
Author(s):  
Zakiya Shakir ◽  
Saeed Khan ◽  
Kidon Sung ◽  
Sangeeta Khare ◽  
Ashraf Khan ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Epithelial Cells ◽  
Nalidixic Acid ◽  
Molecular Characterization ◽  
Point Mutation ◽  
Quinolone Resistance ◽  
Missense Mutations ◽  
Content Type ◽  
Toxin Genes

ABSTRACTSixty-three nalidixic acid-resistantAeromonassp. isolates were obtained from imported shrimp. Phylogenetic analysis ofgyrBsequences indicated that 18 wereA. enteropelogenes, 26 wereA. caviae, and 19 wereA. sobria. Double missense mutations in the quinolone resistance-determining region (QRDR) ofgyrAat codon 83 (Ser→Val/Ile) and codon 92 (Leu→Met) coupled with a point mutation ofparCat codon 80 (Ser→Ile/Phe) conferred high levels of quinolone resistance in the isolates. A majority ofA. enteropelogenesandA. caviaestrains harbored toxin genes, whereas only a fewA. sobriastrains harbored these genes. The fluoroquinolone-resistantAeromonasspp. exhibited higher cytotoxicity than fluoroquinolone-sensitive, virulentAeromonasspp. to rat epithelial cells.

Morphological, histological and molecular characterization of Myxidium cf. rhodei infecting the kidney of Rutilus rutilus

2020 ◽  
Vol 141 ◽  
pp. 39-46
Author(s):  
MD Dorjievna Batueva ◽  
X Pan ◽  
J Zhang ◽  
X Liu ◽  
W Wei ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Rutilus Rutilus ◽  
Longitudinal Axis ◽  
Suture Line ◽  
Present Species ◽  
Supplementary Data

In the present study, we provide supplementary data for Myxidium cf. rhodei Léger, 1905 based on morphological, histological and molecular characterization. M. cf. rhodei was observed in the kidneys of 918 out of 942 (97%) roach Rutilus rutilus (Linnaeus, 1758). Myxospores of M. cf. rhodei were fusiform with pointed ends, measuring 12.7 ± 0.1 SD (11.8-13.4) µm in length and 4.6 ± 0.1 (3.8-5.4) µm in width. Two similar pear-shaped polar capsules were positioned at either ends of the longitudinal axis of the myxospore: each of these capsules measured 4.0 ± 0.1 (3.1-4.7) µm in length and 2.8 ± 0.1 (2.0-4.0) µm in width. Polar filaments were coiled into 4 to 5 turns. Approximately 18-20 longitudinal straight ridges were observed on the myxospore surface. The suture line was straight and distinctive, running near the middle of the valves. Histologically, the plasmodia of the present species were found in the Bowman’s capsules, and rarely in the interstitium of the host. Phylogenetic analysis revealed that M. cf. rhodei was sister to M. anatidum in the Myxidium clade including most Myxidium species from freshwater hosts.

scholarly journals Etiological Work-Up for Adults with Bronchiectasis: A Predictive Diagnostic Score for Primary Ciliary Dyskinesia and Cystic Fibrosis

2021 ◽  
Vol 10 (16) ◽  
pp. 3478
Author(s):  
Frederic Schlemmer ◽  
Agnes Hamzaoui ◽  
Sonia Zebachi ◽  
Aurelie Le Thuaut ◽  
Gilles Mangiapan ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Primary Ciliary Dyskinesia ◽  
Clinical Score ◽  
Adult Patients ◽  
Operating Characteristics ◽  
Diagnostic Score ◽  
Receiver Operating Characteristics Curve ◽  
Diagnostic Work ◽  
Work Up ◽  
Ciliary Dyskinesia

Background: etiological investigations are not done for all adult patients with bronchiectasis because of the availability and interpretation of tests. The aim of the study was to elaborate a score to identify patients at high risk of having cystic fibrosis or primary ciliary dyskinesia (CF/PCD), which require appropriate management. Methods: diagnostic work-ups were carried out on a French monocenter cohort, and results were subjected to logistic-regression analyses to identify the independent factors associated with CF/PCD diagnosis and, thereby, elaborate a score to validate in a second cohort. Results: among 188 patients, 158 had no obvious diagnosis and were enrolled in the algorithm-construction group. In multivariate analyses, age at symptom onset (8.69 (2.10–35.99); p = 0.003), chronic ENT symptoms or diagnosed sinusitis (10.53 (1.26–87.57); p = 0.03), digestive symptoms or situs inversus (5.10 (1.23–21.14); p = 0.025), and Pseudomonas. aeruginosa and/or Staphylococcus aureus isolated from sputum (11.13 (1.34–92.21); p = 0.02) are associated with CF or PCD. Receiver operating characteristics curve analysis, using a validation group of 167 patients with bronchiectasis, confirmed the score’s performance with AUC 0.92 (95% CI: 0.84–0.98). Conclusions: a clinical score may help identify adult patients with bronchiectasis at higher risk of having CF or PCD.

scholarly journals P096 Long-term follow-up of adolescent and adult patients with cystic fibrosis: a single centre's experience

2021 ◽  
Vol 20 ◽  
pp. S68
Author(s):  
A.I. Yilmaz ◽  
G. Ünal ◽  
B.S. Kibar ◽  
P. Sevgi ◽  
O. Eĝil ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Adult Patients ◽  
Long Term Follow Up

scholarly journals 205 Folate deficiency in adult patients at a regional cystic fibrosis (CF) centre

2014 ◽  
Vol 13 ◽  
pp. S99
Author(s):  
L. Cassidy ◽  
A. Morton ◽  
C. Etherington ◽  
D. Peckham
Keyword(s):  
Cystic Fibrosis ◽  
Folate Deficiency ◽  
Adult Patients

scholarly journals Molecular Characterization of Achromobacter Isolates from Cystic Fibrosis and Non-Cystic Fibrosis Patients in Madrid, Spain

2013 ◽  
Vol 51 (6) ◽  
pp. 1927-1930 ◽  
Author(s):  
L. Barrado ◽  
P. Branas ◽  
M. A. Orellana ◽  
M. T. Martinez ◽  
G. Garcia ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Molecular Characterization
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