scholarly journals HIV-1 drug resistance testing from dried blood spots collected in rural Tanzania using the ViroSeq HIV-1 Genotyping System

2010 ◽  
Vol 66 (2) ◽  
pp. 260-264 ◽  
Author(s):  
A. Johannessen ◽  
C. Garrido ◽  
N. Zahonero ◽  
E. Naman ◽  
C. de Mendoza
Keyword(s):  
Drug Resistance ◽  
Dried Blood Spots ◽  
Resistance Testing ◽  
Blood Spots ◽  
Drug Resistance Testing ◽  
Rural Tanzania ◽  
Hiv 1

HIV type-1 drug resistance testing on dried blood spots is feasible and reliable in patients who fail antiretroviral therapy in rural Tanzania

2010 ◽  
Vol 15 (7) ◽  
pp. 1003-1009 ◽  
Author(s):  
Asgeir Johannessen ◽  
Mona Holberg-Petersen ◽  
Gunilla Lövgaarden ◽  
Ezra Naman ◽  
Vidar Ormaasen ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antiretroviral Therapy ◽  
Dried Blood Spots ◽  
Resistance Testing ◽  
Blood Spots ◽  
Drug Resistance Testing ◽  
Rural Tanzania ◽  
Hiv Type 1

scholarly journals HIV-2 Drug Resistance Genotyping from Dried Blood Spots

2020 ◽  
Vol 59 (1) ◽  
pp. e02303-20
Author(s):  
Dana N. Raugi ◽  
Robert S. Nixon ◽  
Sally Leong ◽  
Khadim Faye ◽  
Jean Phillipe Diatta ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Viral Load ◽  
Plasma Viral Load ◽  
Mononuclear Cells ◽  
Dried Blood Spots ◽  
Antiretroviral Drugs ◽  
Resistance Testing ◽  
Peripheral Blood Mononuclear ◽  
Blood Spots ◽  
Drug Resistance Testing

ABSTRACTThe treatment of HIV-2 in resource-limited settings (RLS) is complicated by the limited availability of HIV-2-active antiretroviral drugs and inadequate access to HIV-2 viral load and drug resistance testing. Dried blood spots (DBS)-based drug resistance testing, widely studied for HIV-1, has not been reported for HIV-2 and could present an opportunity to improve care for HIV-2-infected individuals. We selected 150 DBS specimens from ongoing studies of antiretroviral therapy (ART) for HIV-2 infection in Senegal and subjected them to genotypic drug resistance testing. Total nucleic acid was extracted from DBS, reverse transcribed, PCR amplified, and analyzed by population-based Sanger sequencing, and major drug resistance-associated mutations (RAM) were identified. Parallel samples from plasma and peripheral blood mononuclear cells (PBMC) were also genotyped. We obtained 58 protease/reverse transcriptase genotypes. Plasma viral load was significantly correlated with genotyping success (P < 0.001); DBS samples with corresponding plasma viral load >250 copies/ml had a success rate of 86.8%. In paired DBS-plasma genotypes, 83.8% of RAM found in plasma were also found in DBS, and replicate DBS genotyping revealed that a single test detected 86.7% of known RAM. These findings demonstrate that DBS-based genotypic drug resistance testing for HIV-2 is feasible and can be deployed in RLS with limited infrastructure.

scholarly journals Antiretroviral Drug Resistance Testing in Adult HIV‐1 Infection: 2008 Recommendations of an International AIDS Society–USA Panel

2008 ◽  
Vol 47 (2) ◽  
pp. 266-285 ◽  
Author(s):  
Martin S. Hirsch ◽  
Huldrych F. Günthard ◽  
Jonathan M. Schapiro ◽  
Françoise Brun‐Vézinet ◽  
Bonaventura Clotet ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Resistance Testing ◽  
International Aids ◽  
Antiretroviral Drug Resistance ◽  
Drug Resistance Testing ◽  
Antiretroviral Drug ◽  
Hiv 1

Routine drug resistance testing in HIV-1 proviral DNA, using an automated next- generation sequencing assay

2019 ◽  
Vol 121 ◽  
pp. 104207 ◽  
Author(s):  
Enagnon Kazali Alidjinou ◽  
Pauline Coulon ◽  
Christophe Hallaert ◽  
Olivier Robineau ◽  
Agnès Meybeck ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Next Generation Sequencing ◽  
Resistance Testing ◽  
Next Generation ◽  
Proviral Dna ◽  
Drug Resistance Testing ◽  
Generation Sequencing ◽  
Hiv 1

Introduction of TaqMan HIV-1 Assay Increased Unnecessary Drug Resistance Testing

2010 ◽  
Vol 24 (4) ◽  
pp. 203-204 ◽  
Author(s):  
Hiroyuki Gatanaga ◽  
Minoru Oowa ◽  
Shinichi Oka
Keyword(s):  
Drug Resistance ◽  
Resistance Testing ◽  
Drug Resistance Testing ◽  
Hiv 1

Dried blood spots for HIV-1 drug resistance genotyping in decentralized settings in Senegal

2013 ◽  
Vol 86 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Abou Abdallah Malick Diouara ◽  
Halimatou Diop-Ndiaye ◽  
Khady Kebe-Fall ◽  
Edmond Tchiakpè ◽  
Ousseynou Ndiaye ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Drug Resistance Genotyping ◽  
Hiv 1
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