scholarly journals A 63 kb genomic resistance island found in a multidrug-resistant Acinetobacter baumannii isolate of European clone I from 1977

2010 ◽  
Vol 65 (9) ◽  
pp. 1915-1918 ◽  
Author(s):  
Lenka Krizova ◽  
Alexandr Nemec
Keyword(s):  
Acinetobacter Baumannii ◽  
Multidrug Resistant ◽  
Resistance Island ◽  
Baumannii Isolate

scholarly journals Complete Genome Sequence of a Multidrug-Resistant Acinetobacter baumannii Isolate Obtained from a Mexican Hospital (Sequence Type 422)

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Semiramis Castro-Jaimes ◽  
Abraham David Salgado-Camargo ◽  
Lucía Graña-Miraglia ◽  
Luis Lozano ◽  
Paola Bocanegra-Ibarias ◽  
...  
Keyword(s):  
Intensive Care ◽  
Acinetobacter Baumannii ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Hematologic Malignancies ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Nosocomial Pathogen ◽  
Baumannii Isolate

Acinetobacter baumannii has emerged as a dangerous nosocomial pathogen, particularly for severely ill patients in intensive care units and patients with hematologic malignancies. Here, we present the complete genome sequence of a multidrug-resistant A. baumannii isolate, recovered from a Mexican hospital and classified as sequence type 422 according to the multilocus sequence typing Pasteur scheme.

scholarly journals Real-Time Sequencing To Decipher the Molecular Mechanism of Resistance of a Clinical Pan-Drug-Resistant Acinetobacter baumannii Isolate from Marseille, France

2012 ◽  
Vol 57 (1) ◽  
pp. 592-596 ◽  
Author(s):  
Jean-Marc Rolain ◽  
Seydina M. Diene ◽  
Marie Kempf ◽  
Gregory Gimenez ◽  
Catherine Robert ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Molecular Mechanisms ◽  
Multidrug Resistant ◽  
Drug Resistant ◽  
Two Component System ◽  
Genome Sequences ◽  
Component System ◽  
Content Type ◽  
Two Component ◽  
Baumannii Isolate

ABSTRACTWe compare the whole-genome sequences of two multidrug-resistant clinicalAcinetobacter baumanniiisolates recovered in the same patient before (ABIsac_ColiS susceptible to colistin and rifampin only) and after (ABIsac_ColiR resistant to colistin and rifampin) treatment with colistin and rifampin. We decipher all the molecular mechanisms of antibiotic resistance, and we found mutations in therpoBgene and in the PmrAB two-component system explaining resistance to rifampin and colistin in ABIsac_ColiR, respectively.

scholarly journals First Genome Sequence of a Mexican Multidrug-Resistant Acinetobacter baumannii Isolate

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Lucía Graña-Miraglia ◽  
Luis Lozano ◽  
Semiramis Castro-Jaimes ◽  
Miguel A. Cevallos ◽  
Patricia Volkow ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Mexico City ◽  
Genome Sequence ◽  
Draft Genome ◽  
Tertiary Hospital ◽  
Multidrug Resistant ◽  
Genomic Diversity ◽  
Nosocomial Pathogen ◽  
Starting Point ◽  
Baumannii Isolate

Acinetobacter baumannii has emerged as an important nosocomial pathogen worldwide. Here, we present the draft genome of the first multidrug-resistant A. baumannii isolate, sampled from a tertiary hospital in Mexico City. This genome will provide a starting point for studying the genomic diversity of this species in Mexico.

scholarly journals Genomic Analysis of the Multidrug-Resistant Acinetobacter baumannii Strain MDR-ZJ06 Widely Spread in China

2011 ◽  
Vol 55 (10) ◽  
pp. 4506-4512 ◽  
Author(s):  
Hua Zhou ◽  
Tongwu Zhang ◽  
Dongliang Yu ◽  
Borui Pi ◽  
Qing Yang ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Genomic Analysis ◽  
Multidrug Resistant ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Resistance Island

ABSTRACTWe previously reported that the multidrug-resistant (MDR)Acinetobacter baumanniistrain MDR-ZJ06, belonging to European clone II, was widely spread in China. In this study, we report the whole-genome sequence of this clinically important strain. A 38.6-kb AbaR-type genomic resistance island (AbaR22) was identified in MDR-ZJ06. AbaR22 has a structure similar to those of the resistance islands found inA. baumanniistrains AYE and AB0057, but it contained only a few antibiotic resistance genes. The region of resistant gene accumulation as previously described was not found in AbaR22. In the chromosome of the strain MDR-ZJ06, we identified the geneblaoxa-23in a composite transposon (Tn2009). Tn2009shared the backbone with otherA. baumanniitransponsons that harborblaoxa-23, but it was bracketed by two ISAba1elements which were transcribed in the same orientation. MDR-ZJ06 also expressed thearmAgene on its plasmid pZJ06, and this gene has the same genetic environment as thearmAgene of theEnterobacteriaceae. These results suggest variability of resistance acquisition even in closely relatedA. baumanniistrains.

scholarly journals Class 1 integron element in Thai Acinetobacter baumannii reveals a linkage to the European clone I

2012 ◽  
Vol 1 (1) ◽  
pp. 24-28
Author(s):  
B Thapa ◽  
C Tribuddharat ◽  
S Srifuengfung ◽  
C Dhiraputra
Keyword(s):  
Acinetobacter Baumannii ◽  
Blot Hybridization ◽  
Variable Region ◽  
Southern Blot Hybridization ◽  
Multidrug Resistant ◽  
Dot Blot ◽  
Class 1 Integron ◽  
Integrase Gene ◽  
Class 1 ◽  
Resistance Island

BACKGROUND: Class 1 integron element is innate to most of the multidrug resistant Acinetobacter baumannii and its spread is common among international clones worldwide. The aim of this study was to document the presence of blaVEB-1 harboring class 1 integron element and its gene cassettes in Thai A. baumannii in relation to A. baumannii European clone I, AYE strain. MATERIALS AND METHODS: Thirty seven carbapenem resistant A. baumannii isolates identified in routine microbiology laboratory of Siriraj Hospital, Bangkok were studied. The dot blot hybridization was performed to detect class 1 integron element integrase gene. PCR was used to amplify blaVEB-1, arr2, cmlA, blaOXA-10 resistance cassettes, and variable region of class 1 integron element. blaVEB-1 gene was localized by southern blot hybridization. RESULTS: The prevalence of class 1 integron element was 86.48% in the isolates studied. The blaVEB-1 was present in 7 isolates however the location of blaVEB-1 gene was different in different isolate. Four isolates (Ab03-168, Ab04-28, Ab08-20, and Ab08-22) harbored calss 1 integron element variable region sized 5.5 kb as described in strain AYE. However, blaVEB-1 was only amplified from Ab03-168. The cassette organization in this isolate was 5’CS-aadB-blaVEB-1-arr2-cmlA-blaOXA- 10-aadA1-3’CS. The class 1 integron element similar to the element identified in genomic resistance island, AbaRI of European clone I, AYE was identified in Thai A. baumannii. CONCLUSIONS: blaVEB-1 harboring class 1 integron element with minor cassette variation was identified in Thai A. baumanni isolate which might suggest the spread of this resistant cassette or the spread of the European clone I in Thailand. Monitoring of the global spread of multi-resistant A. baumannii is mandatory to control the spread of resistant genes and this multi-resistant pathogen. DOI: http://dx.doi.org/10.3126/ijim.v1i1.6715Int J Infect Microbiol 2012;1(1):24-28

scholarly journals Comparative Genome Sequence Analysis of Multidrug-Resistant Acinetobacter baumannii

2008 ◽  
Vol 190 (24) ◽  
pp. 8053-8064 ◽  
Author(s):  
Mark D. Adams ◽  
Karrie Goglin ◽  
Neil Molyneaux ◽  
Kristine M. Hujer ◽  
Heather Lavender ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Multidrug Resistant ◽  
Drug Efflux ◽  
Acinetobacter Baylyi ◽  
Genome Sequences ◽  
Genome Sequence Analysis ◽  
Health Care Settings ◽  
Resistance Determinants ◽  
Recent Emergence ◽  
Resistance Island

ABSTRACT The recent emergence of multidrug resistance (MDR) in Acinetobacter baumannii has raised concern in health care settings worldwide. In order to understand the repertoire of resistance determinants and their organization and origins, we compared the genome sequences of three MDR and three drug-susceptible A. baumannii isolates. The entire MDR phenotype can be explained by the acquisition of discrete resistance determinants distributed throughout the genome. A comparison of closely related MDR and drug-susceptible isolates suggests that drug efflux may be a less significant contributor to resistance to certain classes of antibiotics than inactivation enzymes are. A resistance island with a variable composition of resistance determinants interspersed with transposons, integrons, and other mobile genetic elements is a significant but not universal contributor to the MDR phenotype. Four hundred seventy-five genes are shared among all six clinical isolates but absent from the related environmental species Acinetobacter baylyi ADP1. These genes are enriched for transcription factors and transporters and suggest physiological features of A. baumannii that are related to adaptation for growth in association with humans.

scholarly journals Deletion of TnAbaR23Results in both Expected and Unexpected Antibiogram Changes in a Multidrug-Resistant Acinetobacter baumannii Strain

2012 ◽  
Vol 56 (4) ◽  
pp. 1845-1853 ◽  
Author(s):  
Mandira Kochar ◽  
Marialuisa Crosatti ◽  
Ewan M. Harrison ◽  
Barbara Rieck ◽  
Jacqueline Chan ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Multidrug Resistant ◽  
En Bloc ◽  
Content Type ◽  
Targeted Deletion ◽  
Resistance Island ◽  
A Minor ◽  
Associated Function ◽  
Similar Elements ◽  
Increased Susceptibility

ABSTRACTSince the 2006 discovery of theAcinetobacter baumanniistrain AYE AbaR1 resistance island, similar elements have been reported in numerous members of this species. As AbaR1 is distantly related to Tn7, we have renamed it TnAbaR1. TnAbaRtransposons are known to carry multiple antibiotic resistance- and efflux-associated genes, although none have been experimentally studieden bloc. We deleted the TnAbaRtransposon inA. baumanniiA424, which we have designated TnAbaR23, and characterized independent deletion mutants DCO163 and DCO174. The NotI pulsed-field gel electrophoresis (PFGE) profile of strain DCO174 was consistent with targeted deletion of TnAbaR23alone, but strain DCO163 apparently harbored a second large genomic deletion. Nevertheless, “subtractive amplification” targeting 52 TnAbaRand/or resistance-associated loci yielded identical results for both mutants and highlighted genes lost relative to strain A424. PCR mapping and genome sequencing revealed the entire 48.3-kb sequence of TnAbaR23. Consistent with TnAbaR23carrying two copies ofsul1, both mutants exhibited markedly increased susceptibility to sulfamethoxazole. In contrast, loss oftetAR(A) resulted in only a minor and variable increase in tetracycline susceptibility. Despite not exhibiting a growth handicap, strain DCO163 was more susceptible than strain DCO174 to 9 of 10 antibiotics associated with mutant-to-mutant variation in susceptibility, suggesting impairment of an undefined resistance-associated function. Remarkably, despite all three strains sharing identicalgyrAandparCsequences, the ciprofloxacin MIC of DCO174 was >8-fold that of DCO163 and A424, suggesting a possible paradoxical role for TnAbaR23in promoting sensitivity to ciprofloxacin. This study highlights the importance of experimental scrutiny and challenges the assumption that resistance phenotypes can reliably be predicted from genotypes alone.

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