scholarly journals Molecular characterization of macrolide- and multidrug-resistant Streptococcus pyogenes isolated from adult patients in Barcelona, Spain (1993-2008)

2010 ◽  
Vol 65 (4) ◽  
pp. 634-643 ◽  
Author(s):  
C. Ardanuy ◽  
A. Domenech ◽  
D. Rolo ◽  
L. Calatayud ◽  
F. Tubau ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Streptococcus Pyogenes ◽  
Multidrug Resistant ◽  
Adult Patients

scholarly journals Isolation, Identification and Molecular Characterization of Nocardiopsaceae Producing Novel Antibiotics From Soil Samples in Bingol, Turkey

2020 ◽  
Vol 11 (2) ◽  
pp. 94
Author(s):  
Samer Abuzerr ◽  
Kate Zinszer ◽  
Syamand Ahmed Qadir ◽  
Ekrem Atalan ◽  
Halgord Ali M. Farag ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Gene Sequence ◽  
Multidrug Resistant ◽  
Physicochemical Analysis ◽  
Soil Samples ◽  
Morphological Properties ◽  
16S Rdna Gene ◽  
Life Threatening ◽  
Immense Potential

There is growing interest in the antimicrobial discovery of life-threatening multidrug-resistant pathogens. The study was undertaken to isolate, identify, and characterize antibiotic-producing actinomycetaceae, particularly nocadisosaceae, from soil samples of Bingol, Turkey. Soil samples were collected from three different regions of Bingol, Turkey. The physicochemical analysis of the soil samples was immediately measured using standard methods. This was followed by isolation of Nocardiopsaceae, nutritional tests, chemotaxonomic analysis, and molecular characterization. The isolated organisms showed morphological properties consistent with the Nocardiopsaceae soil bacteria.  The 16s rDNA gene sequence indicated a similarity between the strains with 99.86% which was Nocardiopsaceae synnemata-formans. The BLAST hits had a significant e-value of 0.005. The results of the present study revealed that soil Nocardiopsaceae of Bingol appears to have immense potential as a source of antibacterial compounds.

Molecular Characterization of Streptococcus pyogenes Causing Invasive Disease in Pediatric Population in Spain A 12-year Study

2019 ◽  
Vol 38 (12) ◽  
pp. 1168-1172
Author(s):  
Viviana Sánchez-Encinales ◽  
Guillermo Ludwig ◽  
Esther Tamayo ◽  
Jose Maria García-Arenzana ◽  
Carmen Muñoz-Almagro ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Streptococcus Pyogenes ◽  
Pediatric Population ◽  
Invasive Disease

scholarly journals Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis Isolates from China

2014 ◽  
Vol 58 (4) ◽  
pp. 1997-2005 ◽  
Author(s):  
Li-Li Zhao ◽  
Yan Chen ◽  
Hai-Can Liu ◽  
Qiang Xia ◽  
Xiao-Cui Wu ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Multidrug Resistant ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Beijing Family ◽  
Phenotypic Data ◽  
Resistant Tuberculosis ◽  
Multidrug Resistant Tuberculosis ◽  
Mdr Tb

ABSTRACTTo investigate the molecular characterization of multidrug-resistant tuberculosis (MDR-TB) isolates from China and the association of specific mutations conferring drug resistance with strains of different genotypes, we performed spoligotyping and sequenced nine loci (katG,inhA, theoxyR-ahpCintergenic region,rpoB,tlyA,eis,rrs,gyrA, andgyrB) for 128 MDR-TB isolates. Our results showed that 108 isolates (84.4%) were Beijing family strains, 64 (59.3%) of which were identified as modern Beijing strains. Compared with the phenotypic data, the sensitivity and specificity of DNA sequencing were 89.1% and 100.0%, respectively, for isoniazid (INH) resistance, 93.8% and 100.0% for rifampin (RIF) resistance, 60.0% and 99.4% for capreomycin (CAP) resistance, 84.6% and 99.4% for kanamycin (KAN) resistance, and 90.0% and 100.0% for ofloxacin (OFX) resistance. The most prevalent mutations among the MDR-TB isolates werekatG315,inhA15,rpoB531, -526, and -516,rrs1401,eis-10, andgyrA94, -90, and -91. Furthermore, there was no association between specific resistance-conferring mutations and the strain genotype. These findings will be helpful for the establishment of rapid molecular diagnostic methods to be implemented in China.

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