scholarly journals In vitro selection of Plasmodium falciparum drug-resistant parasite lines

2009 ◽  
Vol 65 (3) ◽  
pp. 390-398 ◽  
Author(s):  
A. Nzila ◽  
L. Mwai
Keyword(s):  
Plasmodium Falciparum ◽  
In Vitro Selection ◽  
Drug Resistant ◽  
Resistant Parasite ◽  
Drug Resistant Parasite ◽  
Selection Of

In vitro selection of Plasmodium falciparum 3D7 for expression of variant surface antigens associated with severe malaria in African children

2003 ◽  
Vol 25 (8-9) ◽  
pp. 421-427 ◽  
Author(s):  
Trine Staalsoe ◽  
Morten A. Nielsen ◽  
Lasse S. Vestergaard ◽  
Anja T. R. Jensen ◽  
Thor G. Theander ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Severe Malaria ◽  
In Vitro Selection ◽  
Surface Antigens ◽  
Variant Surface Antigens ◽  
African Children ◽  
Selection Of

scholarly journals In vitro selection of Plasmodium falciparum Pfcrt and Pfmdr1 variants by artemisinin

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Muturi J. Njokah ◽  
Joseph N. Kang’ethe ◽  
Johnson Kinyua ◽  
Daniel Kariuki ◽  
Francis T. Kimani
Keyword(s):  
Plasmodium Falciparum ◽  
In Vitro Selection ◽  
Selection Of

scholarly journals In vitro selection of Remdesivir resistance suggests evolutionary predictability of SARS-CoV-2

2021 ◽  
Vol 17 (9) ◽  
pp. e1009929
Author(s):  
Agnieszka M. Szemiel ◽  
Andres Merits ◽  
Richard J. Orton ◽  
Oscar A. MacLean ◽  
Rute Maria Pinto ◽  
...  
Keyword(s):  
Nucleoside Analogue ◽  
Reverse Genetics ◽  
High Throughput Sequencing ◽  
In Vitro Selection ◽  
Single Mutation ◽  
Drug Resistant ◽  
Immune Selection ◽  
Virus Surveillance ◽  
Selection Of

Remdesivir (RDV), a broadly acting nucleoside analogue, is the only FDA approved small molecule antiviral for the treatment of COVID-19 patients. To date, there are no reports identifying SARS-CoV-2 RDV resistance in patients, animal models or in vitro. Here, we selected drug-resistant viral populations by serially passaging SARS-CoV-2 in vitro in the presence of RDV. Using high throughput sequencing, we identified a single mutation in RNA-dependent RNA polymerase (NSP12) at a residue conserved among all coronaviruses in two independently evolved populations displaying decreased RDV sensitivity. Introduction of the NSP12 E802D mutation into our SARS-CoV-2 reverse genetics backbone confirmed its role in decreasing RDV sensitivity in vitro. Substitution of E802 did not affect viral replication or activity of an alternate nucleoside analogue (EIDD2801) but did affect virus fitness in a competition assay. Analysis of the globally circulating SARS-CoV-2 variants (>800,000 sequences) showed no evidence of widespread transmission of RDV-resistant mutants. Surprisingly, we observed an excess of substitutions in spike at corresponding sites identified in the emerging SARS-CoV-2 variants of concern (i.e., H69, E484, N501, H655) indicating that they can arise in vitro in the absence of immune selection. The identification and characterisation of a drug resistant signature within the SARS-CoV-2 genome has implications for clinical management and virus surveillance.

Purification and in Vitro Selection of Rosette-Positive (R+) and Rosette-Negative (R-) Phenotypes of Knob-Positive Plasmodium falciparum Parasites

1992 ◽  
Vol 46 (4) ◽  
pp. 371-381 ◽  
Author(s):  
Shiroma M. Handunnetti ◽  
Russell J. Howard ◽  
Marie-Rose van Schravendijk ◽  
Kei-Ichiro Nakamura ◽  
Aileen D. Gilladoga ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
In Vitro Selection ◽  
Selection Of

In vitro selection of halofantrine resistance in Plasmodium falciparum is not associated with increased expression of Pgh1

1996 ◽  
Vol 83 (1) ◽  
pp. 35-46 ◽  
Author(s):  
Graeme Y Ritchie ◽  
Mathirut Mungthin ◽  
Janet E Green ◽  
Patrick G Bray ◽  
Shaun R Hawley ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
In Vitro Selection ◽  
Selection Of

In vitro selection of DNA aptamers recognizing drug-resistant ovarian cancer by cell-SELEX

Talanta ◽  
2019 ◽  
Vol 194 ◽  
pp. 437-445 ◽  
Author(s):  
Junqing He ◽  
Junyan Wang ◽  
Nan Zhang ◽  
Luyao Shen ◽  
Linlin Wang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
In Vitro Selection ◽  
Dna Aptamers ◽  
Drug Resistant ◽  
Selection Of
Sign in / Sign up

Export Citation Format

Share Document