scholarly journals Detection of methicillin-resistant Staphylococcus aureus ST398 in food samples of animal origin in Spain

2009 ◽  
Vol 64 (6) ◽  
pp. 1325-1326 ◽  
Author(s):  
C. Lozano ◽  
M. Lopez ◽  
E. Gomez-Sanz ◽  
F. Ruiz-Larrea ◽  
C. Torres ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Food Samples ◽  
Animal Origin ◽  
Methicillin Resistant

Molecular Characterization and Antimicrobial Resistance Profile of Methicillin-Resistant Staphylococcus aureus in Retail Chicken

2015 ◽  
Vol 78 (10) ◽  
pp. 1879-1884 ◽  
Author(s):  
KHALID IBRAHIM SALLAM ◽  
SAMIR MOHAMMED ABD-ELGHANY ◽  
MOHAMED ELHADIDY ◽  
TOMOHIRO TAMURA
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Meca Gene ◽  
Multidrug Resistant ◽  
Animal Origin ◽  
Contamination Rate ◽  
Methicillin Resistant ◽  
Food Of Animal Origin

The emergence of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) in food-producing animals is of increasing interest, raising questions about the presence of MRSA in food of animal origin and potential sources of transmission to humans via the food chain. In this study, the prevalence, molecular characterization, virulence factors, and antimicrobial susceptibility patterns of MRSA isolates from 200 retail raw chicken samples in Egypt were determined. MRSA was detected by positive amplification of the mecA gene in 38% (76 of 200) of chicken samples analyzed. This represents a potential public health threat in Egypt, as this contamination rate seems to be the highest among other studies reported worldwide. Furthermore, genes encoding α-hemolysin (hla) and staphylococcal enterotoxins (sea, seb, and sec) were detected in all of the 288 MRSA isolates. Nonetheless, none of the strains tested carried tst, the gene encoding toxic shock syndrome toxin 1. Antimicrobial resistance of MRSA isolates was most frequently detected against penicillin (93.4%), ampicillin (88.9%), and cloxacillin (83.3%). These results suggest that retail chicken might be a significant potential source for transmission of multidrug-resistant and toxigenic S. aureus in Egypt. This underlines the need for stricter hygienic measures in chicken production in Egypt to minimize the risk of transmission of these strains to consumers. To the best of our knowledge, this is the first study that reports the isolation and molecular characterization of MRSA in retail chicken samples in Egypt.

Methicillin-resistant Staphylococcus aureus (MRSA) in foods of animal origin product in Italy

2007 ◽  
Vol 117 (2) ◽  
pp. 219-222 ◽  
Author(s):  
G. Normanno ◽  
M. Corrente ◽  
G. La Salandra ◽  
A. Dambrosio ◽  
N.C. Quaglia ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Animal Origin ◽  
Methicillin Resistant

P903 Methicillin-resistant Staphylococcus aureus strains from animal origin are recognized by IDI-MRSA PCR

2007 ◽  
Vol 29 ◽  
pp. S234
Author(s):  
R. Roosendaal ◽  
J.A.J.W. Kluytmans ◽  
J.H.C. Woudenberg ◽  
X. Huijsdens ◽  
C.M.J.E. Vandenbroucke-Grauls
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Animal Origin ◽  
Methicillin Resistant

scholarly journals The Prevalence of Staphylococcus aureus and Methicillin Resistant Staphylococcus aureus in Processed Food Samples in Riyadh, Saudi Arabia

2021 ◽  
Author(s):  
Mashael J. Alghizzi ◽  
Maysoon Alansari ◽  
Ashwag Shami
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Saudi Arabia ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sccmec Type ◽  
Protein A ◽  
Food Samples ◽  
Health Concern ◽  
Processed Food ◽  
Methicillin Resistant

Staphylococcus aureus mainly Methicillin Resistant Staphylococcus aureus(MRSA) is a life-threatening infection that occurring in food and caused a public health concern. This study designed to examine the prevalence of S. aureus and MRSA in different types of processed food. Food samples were screened for the recovered strains of S. aureus and MRSA, and they were examined for antimicrobial susceptibility and by molecular characterization of mecA and staphylococcal cassette chromosome mec(SCCmec). Detection of virulence factors like Panton-Valentine Leukocidin (PVL), Staphylococcus aureus protein A(spa) and Staphylococcal enterotoxins(SEs) by PCR using specific primers. Among the 150 collected processed food samples, 62.7% were contaminated by S. aureus bacteria, 56.4% of which were proved as MRSA. 17% of MRSA isolates were positive for mecA genes with the SCCmec type IVb and V (11.1% each) as the solely existing types of SCCmec. None of the MRSA isolates carried mecC or mecB genes. Most of MRSA isolates were multidrug resistance and 33.3% of MRSA-mecA positive isolates also carried vancomycin resistance genes (i.e., vanB). In addition, spa gene was found among 7.5% of MRSA isolates; none of which were positive for PVL gene. Further, there were variant presence of SEs among MRSA isolates and the highest presence was from type SEH (49.1%). Generally, our results confirmed that processed foods in Saudi Arabia (Riyadh) are potential vehicles for multidrug resistant S. aureus and MRSA transmission; which are serious public health risks, and underlined the need for good hygiene practices.

scholarly journals Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia

Antibiotics ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 26 ◽  
Author(s):  
Jelena Asanin ◽  
Dusan Misic ◽  
Ksenija Aksentijevic ◽  
Zoran Tambur ◽  
Bojan Rakonjac ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sccmec Type ◽  
Companion Animals ◽  
Antibiotic Resistance Genes ◽  
Variable Number ◽  
Animal Origin ◽  
Methicillin Resistant ◽  
Genetic Profiling ◽  
Biocide Resistance

The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates—30 obtained from humans and six from companion animals—were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL—Panton–Valentine leukocidin, ETs—exfoliative toxins, TSST—toxic shock syndrome toxin, SEs—staphylococcal enterotoxins, and MSCRAMMs—microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6′)-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and catpC221. All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.

Prevalence and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus in Foods of Animal Origin, Turkey

2016 ◽  
Vol 79 (11) ◽  
pp. 1990-1994 ◽  
Author(s):  
BELGİN SİIRİKEN ◽  
TUBA YILDIRIM ◽  
AKİF KORAY GÜNEY ◽  
İRFAN EROL ◽  
BELMA DURUPINAR
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Raw Milk ◽  
Animal Origin ◽  
Methicillin Resistant ◽  
Selection For

ABSTRACT In the present study, 175 coagulase-positive Staphylococcus (CPS) isolates recovered from samples of beef (n = 110), raw milk n = 56), and fish (n = 9) were analyzed for methicillin resistance using MIC and PCR assays. Methicillin-resistant (MR) Staphylococcus aureus (SA) isolates were then characterized using pulsed-field gel electrophoresis (PFGE). According to findings, 62 (35.4%) of the isolates (44 from beef, 9 from milk, and 9 from fish) were identified as S. aureus based on the presence of the nuc gene. MRCPS was detected in 18 (10.3%) of 175 CPS isolates based on the presence of the mecA gene. Among these isolates, 15 (24.2%) were MRSA: 4 (26.7%) from beef, 2 (13.3%) from milk, and 9 (60%) from fish. However, based on the MIC assay, 21 (12.0%) of the CPS isolates (1 from beef, 15 from milk, and 5 from fish) were MRCPS, indicating a discrepancy between the results of these two methods. The PFGE results indicated genetic heterogeneity of the isolates; six PFGE clusters were found. These results confirm that MRSA is present in foods of animal origin, which is a concern to human health, and indicate the importance of method selection for determination of methicillin resistance. The identity of MR isolates should be verified by PCR to obtain more reliable results.

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