scholarly journals Phenotypic and genetic characterization of the 2008 WHO Neisseria gonorrhoeae reference strain panel intended for global quality assurance and quality control of gonococcal antimicrobial resistance surveillance for public health purposes

2009 ◽  
Vol 63 (6) ◽  
pp. 1142-1151 ◽  
Author(s):  
M. Unemo ◽  
O. Fasth ◽  
H. Fredlund ◽  
A. Limnios ◽  
J. Tapsall
Keyword(s):  
Public Health ◽  
Quality Control ◽  
Quality Assurance ◽  
Antimicrobial Resistance ◽  
Neisseria Gonorrhoeae ◽  
Reference Strain ◽  
Genetic Characterization ◽  
Global Quality ◽  
Phenotypic And Genetic Characterization

Phenotypic and genetic characterization of antimicrobial resistance in Salmonella serovars isolated from retail meats in Alberta, Canada

2012 ◽  
Vol 32 (1) ◽  
pp. 110-117 ◽  
Author(s):  
Mueen Aslam ◽  
Sylvia Checkley ◽  
Brent Avery ◽  
Gabhan Chalmers ◽  
Valerie Bohaychuk ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Genetic Characterization ◽  
Retail Meats ◽  
Salmonella Serovars ◽  
Phenotypic And Genetic Characterization

scholarly journals Phenotypic and genetic characterization of a patient with a de novo interstitial 14q24.1q24.3 deletion

2014 ◽  
Vol 7 (1) ◽  
pp. 49 ◽  
Author(s):  
Elisa Tassano ◽  
Andrea Accogli ◽  
Serena Panigada ◽  
Patrizia Ronchetto ◽  
Cristina Cuoco ◽  
...  
Keyword(s):  
De Novo ◽  
Genetic Characterization ◽  
Phenotypic And Genetic Characterization

Preliminary Phenotypic and Genetic Characterization of 18 Chilean Families Affected by Amelogenesis Imperfecta

2015 ◽  
Vol 119 (3) ◽  
pp. e202-e203
Author(s):  
Blanca Urzúa Orellana ◽  
Irene Morales Bozo ◽  
Ana Ortega Pinto ◽  
Daniela Adorno Farias ◽  
Lilian Jara Sosa ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Amelogenesis Imperfecta ◽  
Phenotypic And Genetic Characterization

Phenotypic and genetic characterization of the Italian bantam chicken breed Mericanel della Brianza

2017 ◽  
Vol 205 ◽  
pp. 56-63 ◽  
Author(s):  
Maria Cristina Cozzi ◽  
Elena Colombo ◽  
Luisa Zaniboni ◽  
Manuela Madeddu ◽  
Fabio Mosca ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Chicken Breed ◽  
Phenotypic And Genetic Characterization

scholarly journals Antimicrobial resistance and genetic characterization of coagulase-negative staphylococci from bovine mastitis milk samples in Korea

2019 ◽  
Vol 102 (12) ◽  
pp. 11439-11448 ◽  
Author(s):  
Su-Jeong Kim ◽  
Dong Chan Moon ◽  
Seung-Chun Park ◽  
Hee Young Kang ◽  
Seok Hyeon Na ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Genetic Characterization ◽  
Bovine Mastitis ◽  
Coagulase Negative Staphylococci ◽  
Milk Samples

scholarly journals Genotypic and Phenotypic Characterization of Antimicrobial Resistance in Neisseria gonorrhoeae: a Cross-Sectional Study of Isolates Recovered from Routine Urine Cultures in a High-Incidence Setting

mSphere ◽  
2019 ◽  
Vol 4 (4) ◽  
Author(s):  
Adam L. Bailey ◽  
Robert F. Potter ◽  
Meghan A. Wallace ◽  
Caitlin Johnson ◽  
Gautam Dantas ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Neisseria Gonorrhoeae ◽  
Susceptibility Testing ◽  
Clinical Laboratory ◽  
Phenotypic Characterization ◽  
First Line ◽  
Content Type ◽  
Gradient Diffusion

ABSTRACT The objectives of this study were to perform genomic and phenotypic characterization of antimicrobial resistance in Neisseria gonorrhoeae isolates recovered from urine samples from patients in St. Louis, MO, USA. Sixty-four clinical isolates were banked over a 2-year period and subjected to antimicrobial susceptibility testing (AST) by Kirby-Bauer disk diffusion (penicillin, tetracycline, cefuroxime, and ciprofloxacin) and gradient diffusion (tetracycline, doxycycline, azithromycin, ceftriaxone, cefixime, ciprofloxacin, gemifloxacin, and delafloxacin). The medical records for the patients were evaluated to determine the demographics, location, and prescribed treatment regimen. Isolate draft genomes were assembled from Illumina shotgun sequencing data, and resistance determinants were identified by ResFinder and PointFinder. Of the 64 isolates, 97% were nonsusceptible to penicillin, with resistant isolates all containing the blaTEM-1b gene; 78 and 81% of isolates were nonsusceptible to tetracycline and doxycycline, respectively, with resistant isolates all containing the tet(M) gene. One isolate was classified as non-wild-type to azithromycin, and all isolates were susceptible to ceftriaxone; 89% of patients received this combination of drugs as first-line therapy. Six percent of isolates were resistant to ciprofloxacin, with most resistant isolates containing multiple gyrA and parC mutations. Correlation between disk and gradient diffusion AST devices was high for tetracycline and ciprofloxacin (R2 > 99% for both). The rates of N. gonorrhoeae antibiotic resistance in St. Louis are comparable to current rates reported nationally, except ciprofloxacin resistance was less common in our cohort. Strong associations between specific genetic markers and phenotypic susceptibility testing hold promise for the utility of genotype-based diagnostic assays to guide directed antibiotic therapy. IMPORTANCE Neisseria gonorrhoeae causes the sexually transmitted infection gonorrhea, which is most commonly diagnosed using a DNA-based detection method that does not require growth and isolation of N. gonorrhoeae in the laboratory. This is problematic because the rates of antibiotic resistance in N. gonorrhoeae are increasing, but without isolating the organism in the clinical laboratory, antibiotic susceptibility testing cannot be performed on strains recovered from clinical specimens. We observed an increase in the frequency of urine cultures growing N. gonorrhoeae after we implemented a total laboratory automation system for culture in our clinical laboratory. Here, we report on the rates of resistance to multiple historically used, first-line, and potential future-use antibiotics for 64 N. gonorrhoeae isolates. We found that the rates of antibiotic resistance in our isolates were comparable to national rates. Additionally, resistance to specific antibiotics correlated closely with the presence of genetic resistance genes, suggesting that DNA-based tests could also be designed to guide antibiotic therapy for treating gonorrhea.

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