scholarly journals Outbreak of hospital-adapted clonal complex-17 vancomycin-resistant Enterococcus faecium strain in a haematology unit: role of rapid typing for early control

2007 ◽  
Vol 60 (4) ◽  
pp. 849-854 ◽  
Author(s):  
A. Deplano ◽  
O. Denis ◽  
C. Nonhoff ◽  
F. Rost ◽  
B. Byl ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Clonal Complex ◽  
Vancomycin Resistant Enterococcus ◽  
Vancomycin Resistant ◽  
Faecium Strain

Identification and characterization of IS1476, an insertion sequence-like element that disrupts VanY function in a vancomycin-resistant Enterococcus faecium strain.

1997 ◽  
Vol 41 (8) ◽  
pp. 1805-1807 ◽  
Author(s):  
M G MacKinnon ◽  
M A Drebot ◽  
G J Tyrrell
Keyword(s):  
Resistance Gene ◽  
Enterococcus Faecium ◽  
Insertion Sequence ◽  
Vancomycin Resistant Enterococcus ◽  
Detection Strategy ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Identification And Characterization ◽  
Faecium Strain

The vanY gene of vancomycin-resistant enterococci encodes a D,D-carboxypeptidase. By using a PCR detection strategy, a VanA Enterococcus faecium clinical isolate was found to have an insertion sequence (IS)-like element designated IS1476 in vanY. The activity of the VanY D,D-carboxypeptidase in this isolate was decreased in a fluorometric fluoraldehyde o-phthalaldehyde assay with diacetyl-L-Lys-D-Ala-D-Ala as the substrate. This, to our knowledge, is the first report of an IS-like element in a vancomycin resistance gene.

scholarly journals Characterization of Bacteriophage against Enterococcus faecium Resistant to Vancomycin Isolated from Chicken Skin

2019 ◽  
pp. 1-14
Author(s):  
Hazzierah Syaffieqah An Nadiah Azlan ◽  
Muhajir Hamid ◽  
Adelene Ai-Lian Song
Keyword(s):  
Latent Period ◽  
Growth Kinetics ◽  
Enterococcus Faecium ◽  
Burst Size ◽  
Single Step ◽  
Vancomycin Resistant Enterococcus ◽  
Chicken Skin ◽  
Step Growth ◽  
Vancomycin Resistant ◽  
Faecium Strain

Aims: To characterize bacteriophages with strong in vitro lytic activity against vancomycin resistant Enterococcus faecium before testing on the chicken skin for their efficacy. Study Design: An experimental was carried out to characterize two isolated bacteriophages against Enterococcus faecium and test for their efficacy on chicken skin. Study Place: The study was carried out in Laboratory of Vaccine and Immunotherapeutics, Institue of Bioscience, Universiti Putra Malaysia in Selangor, which is the most populous state in Malaysia. Methodology: Two host specific lytic phages against vancomycin resistant Enterococcus faecium strain FM8, designated as FM8-P1 and FM8-P2 were physiological characterized. This includes determination of their adsorption rate, multiplicity of infection, and single step growth kinetics. The optimum pH and temperature for both bacteriophages activity were also determined before tested on chicken skin at 4°C and 25°C, which represent chiller and room temperature in poultry production line. Results: Based on the result of single-step growth kinetics, the latent period of FM8-P1 was 35 min with a burst size of 460 particles per infected cells, while FM8-P2 has a shorter latent period (20 min) but a smaller burst size of 60 particles. The highest adsorption rate for FM8-P1 was 83% and FM8-P2 was 90% at 2 min and 4 min respectively. Both bacteriophages also exihibited a wide range of pH and temperature for their activity. Conclusion: The specificity, lytic activity and stability of FM8-P1 and FM8-P2 emphasized their potential in effectively eliminating the vancomycin resistant Enterococcus faecium strain FM8. However, further works are required to validate their in situ reliability.

First report of a linezolid- and vancomycin-resistant Enterococcus faecium strain in Korea

2006 ◽  
Vol 38 (5) ◽  
pp. 383-386 ◽  
Author(s):  
Hae-Gyung Bae ◽  
Heungsup Sung ◽  
Mi-Na Kim ◽  
Eun-Jung Lee ◽  
Sung Koo Lee
Keyword(s):  
Enterococcus Faecium ◽  
Vancomycin Resistant Enterococcus ◽  
First Report ◽  
Vancomycin Resistant ◽  
Faecium Strain

scholarly journals First linezolid- and vancomycin-resistant Enterococcus faecium strain in Taiwan

2005 ◽  
Vol 55 (4) ◽  
pp. 598-599 ◽  
Author(s):  
Chun-Hsing Liao ◽  
Sun-Pin Tseng ◽  
Chi-Tai Fang ◽  
Lee-Jene Teng ◽  
Po-Ren Hsueh
Keyword(s):  
Enterococcus Faecium ◽  
Vancomycin Resistant Enterococcus ◽  
Vancomycin Resistant ◽  
Faecium Strain

scholarly journals Genetic Variability of Vancomycin-Resistant Enterococcus faecium and Enterococcus faecalis Isolates from Humans, Chickens, and Pigs in Malaysia

2013 ◽  
Vol 79 (15) ◽  
pp. 4528-4533 ◽  
Author(s):  
Yitbarek Getachew ◽  
Latiffah Hassan ◽  
Zunita Zakaria ◽  
Saleha Abdul Aziz
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Clonal Complex ◽  
Housekeeping Genes ◽  
Vancomycin Resistant Enterococcus ◽  
Content Type ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Sequence Types ◽  
Representative Samples

ABSTRACTVancomycin-resistant enterococci (VRE) have been reported to be present in humans, chickens, and pigs in Malaysia. In the present study, representative samples of VRE isolated from these populations were examined for similarities and differences by using the multilocus sequence typing (MLST) method. Housekeeping genes ofEnterococcus faecium(n= 14) andEnterococcus faecalis(n= 11) isolates were sequenced and analyzed using the MLST databases eBURST and goeBURST. We found five sequence types (STs) ofE. faeciumand six STs ofE. faecalisexisting in Malaysia.Enterococcus faeciumisolates belonging to ST203, ST17, ST55, ST79, and ST29 were identified, andE. faeciumST203 was the most common among humans. The MLST profiles ofE. faeciumfrom humans in this study were similar to the globally reported nosocomial-related strain lineage belonging to clonal complex 17 (CC17). Isolates from chickens and pigs have few similarities to those from humans, except for one isolate from a chicken, which was identified as ST203.E. faecalisisolates were more diverse and were identified as ST4, ST6, ST87, ST108, ST274, and ST244, which were grouped as specific to the three hosts.E. faecalis, belonging to the high-risk CC2 and CC87, were detected among isolates from humans. In conclusion, even though one isolate from a chicken was found clonal to that of humans, the MLST analysis ofE. faeciumandE. faecalissupports the findings of others who suggest VRE to be predominantly host specific and that clinically important strains are found mainly among humans. The infrequent detection of a human VRE clone in a chicken may in fact suggest a reverse transmission of VRE from humans to animals.

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