scholarly journals In vitro susceptibility of Madurella mycetomatis, prime agent of Madura foot, to tea tree oil and artemisinin

2007 ◽  
Vol 59 (3) ◽  
pp. 553-555 ◽  
Author(s):  
Wendy W. J. van de Sande ◽  
Ahmed H. Fahal ◽  
Thomas V. Riley ◽  
Henri Verbrugh ◽  
Alex van Belkum
Keyword(s):  
Tea Tree Oil ◽  
In Vitro Susceptibility ◽  
Tea Tree ◽  
Madura Foot ◽  
Madurella Mycetomatis

Evaluation of the antibacterial effect of tea tree oil on Enterococcus faecalis and biofilm in vitro

2021 ◽  
pp. 114566
Author(s):  
Jianyan Qi ◽  
Min Gong ◽  
Rui Zhang ◽  
Yumeng Song ◽  
Qian Liu ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Antibacterial Effect ◽  
Tea Tree Oil ◽  
Tea Tree

scholarly journals Emprego do óleo de Melaleuca alternifolia Cheel (Myrtaceae) na odontologia: perspectivas quanto à utilização como antimicrobiano alternativo às doenças infecciosas de origem bucal

2011 ◽  
Vol 13 (4) ◽  
pp. 492-499 ◽  
Author(s):  
A.C.M. Oliveira ◽  
A. Fontana ◽  
T.C. Negrini ◽  
M.N.M. Nogueira ◽  
T.B.L. Bedran ◽  
...  
Keyword(s):  
Tea Tree Oil ◽  
Melaleuca Alternifolia ◽  
Tea Tree

O interesse por medicamentos alternativos, principalmente daqueles provenientes de extratos naturais, tem aumentado nas últimas décadas. A Melaleuca alternifolia é um arbusto pertencente ao gênero Melaleuca, popularmente conhecida como "árvore de chá", cujo principal produto é o óleo essencial (TTO - tea tree oil), de grande importância medicinal por possuir comprovada ação bactericida e antifúngica contra diversos patógenos humanos. Em virtude da atividade terapêutica em diversas especialidades médicas, o TTO passou a ser empregado na área odontológica. Esta revisão de literatura foi realizada com o objetivo de discutir os ensaios já realizados com o TTO contra microrganismos relacionados à doença cárie, doença periodontal e problemas pulpares. O óleo de Melaleuca tem demonstrado boa ação antibacteriana in vitro contra microrganismos bucais, porém, pesquisas envolvendo o estudo do mecanismo de ação sobre as células microbianas ou estudos in vivo ainda são escassos e precisam ser realizados, já que esse produto pode ser útil na odontologia, seja na manutenção química da higiene ou prevenção de doenças bucais.

HERBAL CREAMS OF REISHI EXTRACT AND TEA TREE OIL FOR HIRSUTISM–AN IN VIVO STUDY

2020 ◽  
pp. 106-110
Author(s):  
SANGEETA CHOUDHURY ◽  
BLR MADHAVI
Keyword(s):  
Topical Application ◽  
Hair Growth ◽  
Ethanolic Extract ◽  
Hair Follicles ◽  
In Vivo Studies ◽  
Control Group ◽  
Tea Tree Oil ◽  
Tea Tree

Objective: The aim of this work to formulate, evaluate and compare the effectiveness of herbal creams containing extract of reishi and tea tree oil for treating hirsutism. Methods: Herbal ingredients were authenticated. Cream base was initially formulated. Three formulations of herbal cream were prepared. Reishi ethanolic extract, tea tree oil, and combination of tea tree oil and reishi extract were added to the cream base and formulated cream were named as RHC, THC and RTC respectively. In vitro evaluations on herbal creams were done for the physicochemical characteristics. In vivo studies were carried out on female Swiss Albino mice for the activity against hair growth by topical application of cream to shaved skin. The histological and morphometric evaluation was carried out. Skin irritancy study was conducted. Results: The herbal creams showed desirable physicochemical properties like pH, viscosity and spreadability. Statistical analysis for the length of hair was performed by using one way ANOVA followed by DUNNET’S post hoc test where THC and RTC were found to be significant whereas RHC showed no significant reduction of hair growth compared to control. RTC showed a significant effect at p<0.05 and hair growth reduction was significant for THC at p<0.001 compared to the control group. RTC and THC showed mild to moderate reduction in the size of the hair follicles with a reduction of sebaceous gland size in the histological analysis. Conclusion: Topical application of herbal creams to mice showed that hair growth was fastest in group RHC and was slowest in group THC and intermediate with RTC. It can be concluded that these herbal actives can be used as an effective treatment against hirsutism. Within the study period, tea tree oil was found to be more effective than reishi extract and the combination product. Further formulation studies and in vivo studies need to be carried out on reishi to assess its effectiveness against hirsutism.

In Vitro Activity of Tea Tree Oil Vaginal Suppositories against Candida spp. and Probiotic Vaginal Microbiota

2015 ◽  
Vol 29 (10) ◽  
pp. 1628-1633 ◽  
Author(s):  
Maura Di Vito ◽  
Paola Mattarelli ◽  
Monica Modesto ◽  
Antonietta Girolamo ◽  
Milva Ballardini ◽  
...  
Keyword(s):  
Vaginal Microbiota ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Tea Tree Oil ◽  
Candida Spp ◽  
Tea Tree

scholarly journals Comparison of disc diffusion, E-Test and a modified CLSI broth microdilution method for in vitro susceptibility testing of itraconazole, posaconazole and voriconazole against Madurella mycetomatis

2021 ◽  
Author(s):  
Bertrand Nyuykonge ◽  
Lukas van Amelsvoort ◽  
Kimberly Eadie ◽  
Ahmed H. Fahal ◽  
Annelies Verbon ◽  
...  
Keyword(s):  
Low Income ◽  
Susceptibility Testing ◽  
Fungal Infections ◽  
Disc Diffusion ◽  
Broth Microdilution ◽  
In Vitro Susceptibility ◽  
Bead Beating ◽  
Broth Microdilution Method ◽  
Madurella Mycetomatis

For many fungal infections, in vitro susceptibility testing is used to predict if an isolate is resistant or susceptible to the antifungal agent used to treat the fungal infection. For Madurella mycetomatis , the main causative agent of mycetoma, in vitro susceptibility testing currently is not performed on a routine basis. The current in vitro susceptibility testing method is labor intensive and sonication must be done to generate a hyphal inoculum. For endpoint visualization, expensive viability dyes are needed. Here we investigated if the currently used in vitro susceptibility method could be adapted to make it amendable for use in a routine setting which can be used in low income countries, where mycetoma is endemic. First, we developed a methodology in which hyphal fragments can be generated without the need for sonication, by comparing different bead beating methodologies. Next, in vitro susceptibility was assessed using standard broth microdilution assays as well as disc diffusion, E-testing and VIPcheck™ methodologies. We demonstrate that after a hyphal suspension is generated by glass bead beating, disc diffusion, E-testing and VIPcheck™ can be used to determine susceptibility towards itraconazole, posaconazole and voriconazole of Madurella mycetomatis . The MICs found with the E-test were comparable to those obtained with our modified CLSI-based broth microdilution in vitro susceptibility assay for itraconazole and posaconazole. Furthermore, we found an inverse relationship between the zone of inhibition and MIC obtained with E-test and the modified CLSI broth microdilution technique.

scholarly journals In Vitro Effects of Tea Tree Oil (Melaleuca Alternifolia Essential Oil) and its Principal Component Terpinen-4-ol on Swine Spermatozoa

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1071 ◽  
Author(s):  
Alberto Elmi ◽  
Domenico Ventrella ◽  
Francesca Barone ◽  
Giacomo Carnevali ◽  
Gianfranco Filippini ◽  
...  
Keyword(s):  
Essential Oils ◽  
Membrane Integrity ◽  
Principal Component ◽  
Tea Tree Oil ◽  
Tea Tree ◽  
Beneficial Effects ◽  
In Vitro Effects ◽  
Sensitive Parameters ◽  
Antioxidant Effects

The growing interest towards essential oils stems from their biological capabilities that include antibacterial and antioxidant effects. Such properties may be extremely useful in the reproductive field; nonetheless essential oils show toxic effects that can lead to cell disruption. The present study aimed to evaluate and compare the effects of tea tree oil (TTO) and its principal component terpinen-4-ol (TER) on the morpho-functional parameters of swine spermatozoa. Experimental samples were prepared by suspending 15 × 107 spermatozoa in 5 mL of medium with different concentrations of the above-mentioned compounds: from 0.2 to 2 mg/mL at an interval of 0.2 for TTO, while TER concentrations were adjusted according to its presence in TTO (41.5%). After 3 h incubation at 16 °C, samples were analyzed for pH, viability, acrosome status, and objective motility. The results highlighted a concentration-dependent effect of TTO with total motility as the most sensitive parameter. TER was better tolerated, and the most sensitive parameters were related to membrane integrity, suggesting a different pattern of interaction. The study confirms the importance of evaluating the effects of natural compounds on spermatozoa before exploiting their beneficial effects. Spermatozoa seem to be good candidates for preliminary toxicological screenings in the light of their peculiar properties.

scholarly journals Exploring Additive, Synergistic or Antagonistic Effects of Natural Plant Extracts on In Vitro Beef Feedlot-Type Rumen Microbial Fermentation Conditions

Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 173 ◽  
Author(s):  
Ignacio Fandiño ◽  
Gonzalo Fernandez-Turren ◽  
Alfred Ferret ◽  
Diego Moya ◽  
Lorena Castillejos ◽  
...  
Keyword(s):  
Rumen Fermentation ◽  
Microbial Fermentation ◽  
Tea Tree Oil ◽  
Fermentation Conditions ◽  
Antagonistic Effects ◽  
Tea Tree ◽  
Fermentation Profile ◽  
Clove Bud Oil ◽  
Oregano Oil

Six Essential oils (EO) (tea tree oil—TeTr, oregano oil—Ore, clove bud oil—Clo, thyme oil—Thy, rosemary oil—Ros, and sage oil—Sag) in Experiment 1; and different combinations of selected oils in Experiment 2, were evaluate at four doses in an in vitro microbial fermentation system using ruminal fluid from beef cattle fed a 10:90 straw: Concentrate diet. In Experiment 1, TeTr, Ore, Clo and Thy improved rumen fermentation profile in a direction consistent with better feed utilization. In Experiment 2, TeTr mixed with Thy, Ore, Thy + Ore or Clo at 200 and 400 mg/L increased the molar proportion of propionate and decreased that of acetate, and the acetate to propionate ratio. However, the size of the effect was similar to that obtained with TeTr alone, suggesting that effects were not additive. When Thy, Ore or Thy + Ore where mixed with Clo, most effects on rumen fermentation profile disappeared, suggesting an antagonistic interaction of Clo with Thy and Ore. Results do not support the hypothesis of additivity among the EO tested, and antagonistic effects of Clo mixed with Thy or Ore were demonstrated at least in a low pH, beef-type fermentation conditions.

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