scholarly journals Genotypic versus phenotypic characterization, with respect to susceptibility and identification, of 17 clinical isolates of Staphylococcus lugdunensis

2005 ◽  
Vol 56 (2) ◽  
pp. 287-291 ◽  
Author(s):  
María Mateo ◽  
Juan-Ramón Maestre ◽  
Lorenzo Aguilar ◽  
Fabio Cafini ◽  
Pilar Puente ◽  
...  
Keyword(s):  
Clinical Isolates ◽  
Phenotypic Characterization ◽  
Staphylococcus Lugdunensis

scholarly journals Molecular and phenotypic characterization of clinical isolates belonging to a KPC-2-producing strain of ST15 Klebsiella pneumoniae from a Vietnamese pediatric hospital

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Björn Berglund ◽  
Ngoc Thi Bich Hoang ◽  
Maria Tärnberg ◽  
Ngai Kien Le ◽  
Maud Nilsson ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Resistance Genes ◽  
Resistance Rate ◽  
Clinical Isolates ◽  
Phenotypic Characterization ◽  
Polymorphism Analysis ◽  
Pediatric Hospital ◽  
Carbapenem Resistant ◽  
Global Epidemiology

Abstract Background Carbapenem-resistant Klebsiella pneumoniae are becoming increasingly common in hospital settings worldwide and are a source of increased morbidity, mortality and health care costs. The global epidemiology of carbapenem-resistant K. pneumoniae is characterized by different strains distributed geographically, with the strain ST258 being predominant in Europe and USA, and ST11 being most common in East Asia. ST15 is a less frequently occurring strain but has nevertheless been reported worldwide as a source of hospital outbreaks of carbapenem-resistant K. pneumoniae. Methods In this study, whole-genome sequencing and antimicrobial susceptibility testing was used to characterize 57 clinical isolates of carbapenem-resistant K. pneumoniae belonging to a strain of ST15, which were collected at a Vietnamese pediatric hospital from February throughout September 2015. Results Aside from the carbapenem resistance gene blaKPC-2, which was carried by all isolates, prevalence of resistance genes to other antibiotics including aminoglycosides, macrolides, quinolones, fosfomycin and trimethoprim, was also high. All isolates were multidrug-resistant. Susceptibility was highest to ceftazidime/avibactam (96%), gentamicin (91%) and tigecycline (82%). Notably, the colistin resistance rate was very high (42%). Single-nucleotide polymorphism analysis indicated that most isolates belonged to a single clone. Conclusions The diverse variety of antibiotic resistance genes and the high antibiotic resistance rates to last-resort antibiotics such as carbapenems and colistin, is indicative of a highly adaptable strain. This emphasizes the importance of implementation of infection controls measures, continued monitoring of antibiotic resistance and prudent use of antibiotics to prevent further selection of resistant strains and the emergence of pan-resistant clones.

Genotypic and Phenotypic Characterization of Invasive Neonatal Escherichia coli Clinical Isolates

2014 ◽  
Vol 31 (11) ◽  
pp. 975-982 ◽  
Author(s):  
Salika Shakir ◽  
Jessica Goldbeck ◽  
Denise Robison ◽  
Annette Eckerd ◽  
Susana Chavez-Bueno
Keyword(s):  
Escherichia Coli ◽  
Clinical Isolates ◽  
Phenotypic Characterization

scholarly journals Piperaquine resistant Cambodian Plasmodium falciparum clinical isolates: in vitro genotypic and phenotypic characterization

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Nonlawat Boonyalai ◽  
Brian A. Vesely ◽  
Chatchadaporn Thamnurak ◽  
Chantida Praditpol ◽  
Watcharintorn Fagnark ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Clinical Isolates ◽  
Phenotypic Characterization

scholarly journals Phenotypic characterization of macrolide and lincosamide resistance patterns in clinical isolates of staphylococci

2016 ◽  
Vol 5 (3) ◽  
pp. 187 ◽  
Author(s):  
Vasanti Kabra ◽  
Ribekha Zachariah ◽  
Sreekanth Basireddy ◽  
Manisha Singh ◽  
Salmaan Ali ◽  
...  
Keyword(s):  
Clinical Isolates ◽  
Phenotypic Characterization ◽  
Resistance Patterns

scholarly journals Identification and Characterization of a Novel FosA7 Member from Fosfomycin-Resistant Escherichia coli Clinical Isolates from Canadian Hospitals

2020 ◽  
Vol 65 (1) ◽  
pp. e00865-20
Author(s):  
Kieran A. Milner ◽  
Denice C. Bay ◽  
David Alexander ◽  
Andrew Walkty ◽  
James A. Karlowsky ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Clinical Isolates ◽  
Phenotypic Characterization ◽  
Content Type ◽  
E Coli ◽  
Fosfomycin Resistance ◽  
Identification And Characterization

ABSTRACTHere, we characterize the fosA genes from three Escherichia coli clinical isolates recovered from Canadian patients. Each fosA sequence was individually overexpressed in E. coli BW25113, and antimicrobial susceptibility testing was performed to assess their role in fosfomycin resistance. The findings from this study identify and functionally characterize FosA3, FosA8, and novel FosA7 members and highlight the importance of phenotypic characterization of fosA genes.

scholarly journals Diversity of mucoid to non-mucoid switch among carbapenemase-producing Klebsiella pneumoniae

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Adriana Chiarelli ◽  
Nicolas Cabanel ◽  
Isabelle Rosinski-Chupin ◽  
Pengdbamba Dieudonné Zongo ◽  
Thierry Naas ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Phenotypic Variation ◽  
Point Mutations ◽  
Carbapenem Resistance ◽  
Selective Advantage ◽  
Clinical Isolates ◽  
Phenotypic Characterization ◽  
Colony Development ◽  
Genome Comparison

Abstract Background Klebsiella pneumoniae is a leading cause of intractable hospital-acquired multidrug-resistant infections and carbapenemase-producing K. pneumoniae (CPKp) are particularly feared. Most of the clinical isolates produce capsule as a major virulence factor. Recombination events at the capsule locus are frequent and responsible for capsule diversity within Klebsiella spp. Capsule diversity may also occur within clonal bacterial populations generating differences in colony aspect. However, little is known about this phenomenon of phenotypic variation in CPKp and its consequences. Results Here, we explored the genetic causes of in vitro switching from capsulated, mucoid to non-mucoid, non-capsulated phenotype in eight clinical CPKp isolates. We compared capsulated, mucoid colony variants with one of their non-capsulated, non-mucoid isogenic variant. The two colony variants were distinguished by their appearance on solid medium. Whole genome comparison was used to infer mutations causing phenotypic differences. The frequency of phenotypic switch was strain-dependent and increased along with colony development on plate. We observed, for 72 non-capsulated variants that the loss of the mucoid phenotype correlates with capsule deficiency and diverse genetic events, including transposition of insertion sequences or point mutations, affecting genes belonging to the capsule operon. Reduced or loss of capsular production was associated with various in vitro phenotypic changes, affecting susceptibility to carbapenem but not to colistin, in vitro biofilm formation and autoaggregation. Conclusions The different impact of the phenotypic variation among the eight isolates in terms of capsule content, biofilm production and carbapenem susceptibility suggested heterogeneous selective advantage for capsular loss according to the strain and the mutation. Based on our results, we believe that attention should be paid in the phenotypic characterization of CPKp clinical isolates, particularly of traits related to virulence and carbapenem resistance.

scholarly journals Evaluation of an Immunochromatographic Assay for Rapid Detection of Penicillin-Binding Protein 2a in Human and Animal Staphylococcus intermedius Group, Staphylococcus lugdunensis, and Staphylococcus schleiferi Clinical Isolates

2015 ◽  
Vol 54 (3) ◽  
pp. 745-748 ◽  
Author(s):  
A. R. Arnold ◽  
C.-A. D. Burnham ◽  
B. A. Ford ◽  
S. D. Lawhon ◽  
S. K. McAllister ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Binding Protein ◽  
Clinical Isolates ◽  
Immunochromatographic Assay ◽  
Penicillin Binding Protein ◽  
Beta Lactam ◽  
Staphylococcus Lugdunensis ◽  
Content Type ◽  
Staphylococcus Intermedius ◽  
Detection Assay

The performance of a rapid penicillin-binding protein 2a (PBP2a) detection assay, the Alere PBP2a culture colony test, was evaluated for identification of PBP2a-mediated beta-lactam resistance in human and animal clinical isolates ofStaphylococcus intermediusgroup,Staphylococcus lugdunensis, andStaphylococcus schleiferi. The assay was sensitive and specific, with all PBP2a-negative and PBP2a-positive strains testing negative and positive, respectively.

scholarly journals Phenotypic characterization of Trichosporon species from clinical isolates

2018 ◽  
Vol 6 (3) ◽  
pp. 153-158
Author(s):  
Dr. Premamalini T ◽  
◽  
Dr. Anitha S. ◽  
Dr. Mohana Priya K ◽  
Dr. Anupma Jyoti Kindo ◽  
...  
Keyword(s):  
Clinical Isolates ◽  
Phenotypic Characterization ◽  
Trichosporon Species
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