scholarly journals Tetracycline resistance of Australian Campylobacter jejuni and Campylobacter coli isolates

2005 ◽  
Vol 55 (4) ◽  
pp. 452-460 ◽  
Author(s):  
A. Pratt ◽  
V. Korolik
Keyword(s):  
Campylobacter Jejuni ◽  
Tetracycline Resistance ◽  
Campylobacter Coli

scholarly journals Whole Resistome Analysis in Campylobacter jejuni and C. coli Genomes Available in Public Repositories

2021 ◽  
Vol 12 ◽  
Author(s):  
José F. Cobo-Díaz ◽  
Paloma González del Río ◽  
Avelino Álvarez-Ordóñez
Keyword(s):  
Campylobacter Jejuni ◽  
Tetracycline Resistance ◽  
Multidrug Resistant ◽  
Host Specialization ◽  
Campylobacter Coli ◽  
Geographical Differences ◽  
Clear Trend ◽  
Antimicrobial Resistance Gene ◽  
Public Repositories ◽  
Production Animals

Campylobacter spp. are the most frequent agent of human gastroenteritis worldwide, and the spread of multidrug-resistant strains makes the clinical treatment difficult. The current study presents the resistome analysis of 39,798 Campylobacter jejuni and 11,920 Campylobacter coli genomes available in public repositories. Determinants of resistance to β-lactams (Be) and tetracyclines (Te) were the most frequent for both species, with resistance to quinolones (Qu) as the third most important on C. jejuni and to aminoglycosides (Am) on C. coli. Moreover, resistance to Te, Qu, and Am was frequently found in co-occurrence with resistance to other antibiotic families. Geographical differences on clonal complexes distribution were found for C. jejuni and on resistome genotypes for both C. jejuni and C. coli species. Attending to the resistome patterns by isolation source, three main clusters of genomes were found on C. jejuni genomes at antimicrobial resistance gene level. The first cluster was formed by genomes from human, food production animals (e.g., sheep, cow, and chicken), and food (e.g., dairy products) isolates. The higher incidence of tet(O), associated with tetracycline resistance, and the gyrA (T86I) single-nucleotide polymorphism (SNP), associated with quinolone resistance, among genomes from this cluster could be due to the intense use of these antibiotics in veterinary and human clinical settings. Similarly, a high incidence of tet(O) genes of C. coli genomes from pig, cow, and turkey was found. Moreover, the cluster based on resistome patterns formed by C. jejuni and C. coli genomes of human, turkey, and chicken origin is in agreement with previous observations reporting chicken or poultry-related environments as the main source of human campylobacteriosis infections. Most clonal complexes (CCs) associated with chicken host specialization (e.g., ST-354, ST-573, ST-464, and ST-446) were the CCs with the highest prevalence of determinants of resistance to Be, Qu, and Te. Finally, a clear trend toward an increase in the occurrence of Te and Qu resistance determinants on C. jejuni, linked to the spread of the co-occurrence of the blaOXA–61 and tet(O)-tet(O/W/O) genes and the gyrA (T86I) SNP, was found from 2001 to date in Europe.

scholarly journals Occurrence of tet(O/M/O) Mosaic Gene in Tetracycline-Resistant Campylobacter

2020 ◽  
Vol 8 (11) ◽  
pp. 1710 ◽  
Author(s):  
Lorena Hormeño ◽  
Maria J. Campos ◽  
Santiago Vadillo ◽  
Alberto Quesada
Keyword(s):  
Campylobacter Jejuni ◽  
Inhibitory Concentration ◽  
Elongation Factor ◽  
Tetracycline Resistance ◽  
Foodborne Diseases ◽  
Campylobacter Coli ◽  
Negative Sample ◽  
Resistance Determinants ◽  
Different Origins ◽  
The Eu

Campylobacter is one of the most important microorganisms responsible for foodborne diseases in the EU. In this study, we investigated resistance to tetracycline in 139 Campylobacter jejuni and Campylobacter coli samples isolated from human clinical cases. From these, 110 were resistant to tetracycline, with MIC (minimal inhibitory concentration) varying in a range of 1 to >512 μg/mL, and 109 (78.4%) carried tet(O), a gene that confers resistance to tetracycline through the expression of a protein that confers protection to the ribosome. Amongst the tetracycline-resistant isolates, one C. jejuni (HCC30) was the only tet(O)-negative sample, presenting an MIC of 256 μg/mL. Instead, the mosaic gene tet(O/M/O) was found in HCC30 and, as far as we know, this is the first description of this chimeric gene originating from homologous recombination between tet(O) and tet(M). The previously described mosaic gene tet(O/32/O), also found in Campylobacter, presents a chimeric structure very similar to that of tet(O/M/O), affecting domains II and III of encoded proteins distantly related to the elongation factor G (EF-G). The tet(O/M/O) mosaic gene has been found in nucleotide databases in several genomes of Campylobacter isolated from different origins, indicating its frequent acquisition, even though it can be undetected through screening by PCR with specific tet(O) primers. In this work, we address the improvement of classical PCR to efficiently diagnose the most prevalent tetracycline resistance determinants in Campylobacter, including tet(O/M/O), which should be taken into account in the optimization of campylobacteriosis treatments.

scholarly journals Multidrug-resistant Campylobacter jejuni, Campylobacter coli and Campylobacter lari isolated from asymptomatic school-going children in Kibera slum, Kenya

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 92
Author(s):  
Nduhiu Gitahi ◽  
Peter B. Gathura ◽  
Michael M. Gicheru ◽  
Beautice M. Wandia ◽  
Annika Nordin
Keyword(s):  
Campylobacter Jejuni ◽  
Primary Schools ◽  
Tetracycline Resistance ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Campylobacter Coli ◽  
Specific Pcr ◽  
Stool Samples ◽  
Human Ecosystems ◽  
Campylobacter Lari

Background: The objective of this study was to determine the prevalence of thermophilic Campylobacter spp. in asymptomatic school-going children and establish the antibiotic resistance patterns of the isolates towards the drugs used to treat campylobacteriosis, including macrolides, quinolones and tetracycline. Campylobacter spp. are a leading cause of enteric illness and have only recently shown resistance to antibiotics. Methods: This study isolated Campylobacter spp., including Campylobacter coli, Campylobacter jejuni and Campylobacter lari, in stool samples from asymptomatic school-going children in one of the biggest urban slums in Kenya. The disc diffusion method using EUCAST breakpoints was used to identify antibiotic-resistant isolates, which were further tested for genes encoding for tetracycline resistance using primer-specific polymerase chain reaction. Results: In total, 580 stool samples were collected from 11 primary schools considering both gender and age. Subjecting 294 biochemically characterized Campylobacter spp. isolates to genus-specific PCR, 106 (18.27% of stool samples) isolates were confirmed Campylobacter spp. Out of the 106 isolates, 28 (4.83%) were Campylobacter coli, 44 (7.58%) were Campylobacter jejuni while 11 (1.89%) were Campylobacter lari. Campylobacter jejuni had the highest number of isolates that were multi-drug resistant, with 26 out of the 28 tested isolates being resistant to ciprofloxacin (5 mg), nalidixic acid (30 mg), tetracycline (30 mg) and erythromycin (15 mg). Conclusions: In conclusion, asymptomatic school going children in the study area were found to be carriers of multidrug resistant Campylobacter coli, Campylobacter jejuni and Campylobacter lari at 84%. A one-health approach, which considers overlaps in environment, animals and human ecosystems, is recommended in addressing multidrug resistane in Campylobacter, since animals are the main reservoirs and environmental contamination is evident.

Role of the plasmid-encoded tet(O) gene in tetracycline-resistant clinical isolates of Campylobacter jejuni and Campylobacter coli

2007 ◽  
Vol 56 (6) ◽  
pp. 833-837 ◽  
Author(s):  
Javid Iqbal Dasti ◽  
Uwe Groß ◽  
Sven Pohl ◽  
Raimond Lugert ◽  
Michael Weig ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Campylobacter Jejuni ◽  
Acute Gastroenteritis ◽  
Tetracycline Resistance ◽  
Gene Localization ◽  
Campylobacter Coli ◽  
Low Level ◽  
High Level ◽  
Level Resistance

The prevalence of tetracycline resistance, tetracycline MICs and tet(O) gene localization were investigated in 83 Campylobacter isolates from patients suffering from acute gastroenteritis in Germany. Combined biochemical and molecular markers identified 74 isolates (89 %) as Campylobacter jejuni, including seven atypical isolates that failed to hydrolyse hippurate, and nine isolates (11 %) as Campylobacter coli. Tetracycline resistance was detected in six out of nine Campylobacter coli isolates (67 %) and 13 out of 74 C. jejuni isolates (18 %). Low-level tetracycline resistance was observed for C. coli (MIC 16 μg ml−1 for all strains), whereas C. jejuni showed high-level resistance (MIC >256 μg ml−1 for all strains). Both low- and high-level tetracycline resistance was associated with the presence of the tet(O) gene. In C. jejuni, tet(O) was plasmid-encoded in 54 % of tetracycline-resistant isolates, whereas in C. coli, tet(O) appeared to be located on the chromosome.

scholarly journals Chromosomaltet(O)-Harboring Regions in Campylobacter coli Isolates from Turkeys and Swine

2012 ◽  
Vol 78 (23) ◽  
pp. 8488-8491 ◽  
Author(s):  
M. D. Crespo ◽  
J. W. Olson ◽  
E. Altermann ◽  
R. M. Siletzky ◽  
S. Kathariou
Keyword(s):  
Campylobacter Jejuni ◽  
Tetracycline Resistance ◽  
Gene Cassette ◽  
Resistance Determinant ◽  
Campylobacter Coli ◽  
Transporter Gene ◽  
Content Type ◽  
Citrate Transporter ◽  
Genomic Location

ABSTRACTIn turkey-derivedCampylobacter coliisolates of a unique lineage (cluster II), the tetracycline resistance determinanttet(O) was chromosomal and was part of a gene cassette (transposon) interrupting aCampylobacter jejuni-associated putative citrate transporter gene. In contrast, the swine-derivedC. colistrain 6461 harbored a chromosomaltet(O) in a different genomic location.

scholarly journals Identification of the tetracycline resistance gene, tet(O), in Streptococcus pneumoniae.

1996 ◽  
Vol 40 (12) ◽  
pp. 2891-2893 ◽  
Author(s):  
C A Widdowson ◽  
K P Klugman ◽  
D Hanslo
Keyword(s):  
Streptococcus Pneumoniae ◽  
Resistance Gene ◽  
Campylobacter Jejuni ◽  
Nucleotide Sequence Identity ◽  
Amino Acid Sequence Identity ◽  
Tetracycline Resistance ◽  
Campylobacter Coli ◽  
Tetracycline Resistance Gene ◽  
Sequence Identity ◽  
First Time

Five isolates of Streptococcus pneumoniae resistant to tetracycline but lacking tet(M) were studied. The tetracycline resistance gene, tet(O), was detected for the first time in the pneumococcus. The gene was amplified and sequenced and found to share 99% nucleotide sequence identity and 99, 99, and 98% deduced amino acid sequence identity with the tet(O) resistance genes of Streptococcus mutans, Campylobacter coli, and Campylobacter jejuni, respectively.

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