scholarly journals Antimicrobial resistance amongst isolates of Streptococcus pyogenes and Staphylococcus aureus in the PROTEKT antimicrobial surveillance programme during 1999-2000

2002 ◽  
Vol 50 (suppl 2) ◽  
pp. 9-24 ◽  
Author(s):  
R. Canton ◽  
E. Loza ◽  
M. I. Morosini ◽  
F. Baquero
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Streptococcus Pyogenes ◽  
Surveillance Programme ◽  
Antimicrobial Surveillance ◽  
And Staphylococcus Aureus

scholarly journals Paradigm Shift in Antimicrobial Resistance Pattern of Bacterial Isolates during the COVID-19 Pandemic

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 954
Author(s):  
Vikas Saini ◽  
Charu Jain ◽  
Narendra Pal Singh ◽  
Ahmad Alsulimani ◽  
Chhavi Gupta ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Tertiary Care ◽  
Public Health Problem ◽  
Resistance Pattern ◽  
Coagulase Negative Staphylococcus ◽  
Care Hospital ◽  
Bacterial Isolates ◽  
Hospital Infection Control ◽  
And Staphylococcus Aureus

Antimicrobial resistance (AMR) is an emerging public health problem in modern times and the current COVID-19 pandemic has further exaggerated this problem. Due to bacterial co-infection in COVID-19 cases, an irrational consumption of antibiotics has occurred during the pandemic. This study aimed to observe the COVID-19 patients hospitalized from 1 March 2019 to 31 December 2020 and to evaluate the AMR pattern of bacterial agents isolated. This was a single-center study comprising 494 bacterial isolates (blood and urine) that were obtained from patients with SARS-CoV-2 admitted to the ICU and investigated in the Department of Microbiology of a tertiary care hospital in Delhi, India. Out of the total bacterial isolates, 55.46% were gram negative and 44.53% were gram positive pathogens. Of the blood samples processed, the most common isolates were CoNS (Coagulase Negative Staphylococcus) and Staphylococcus aureus. Amongst the urinary isolates, most common pathogens were Escherichia coli and Staphylococcus aureus. A total of 60% MRSA was observed in urine and blood isolates. Up to 40% increase in AMR was observed amongst these isolates obtained during COVID-19 period compared to pre-COVID-19 times. The overuse of antibiotics gave abundant opportunity for the bacterial pathogens to gradually develop mechanisms and to acquire resistance. Since the dynamics of SARS-COV-2 are unpredictable, a compromise on hospital antibiotic policy may ultimately escalate the burden of drug resistant pathogens in hospitals. A shortage of trained staff during COVID-19 pandemic renders it impossible to maintain these records in places where the entire hospital staff is struggling to save lives. This study highlights the extensive rise in the use of antibiotics for respiratory illness due to COVID-19 compared to antibiotic use prior to COVID-19 in ICUs. The regular prescription audit followed by a constant surveillance of hospital infection control practices by the dedicated teams and training of clinicians can improve the quality of medications in the long run and help to fight the menace of AMR.

scholarly journals Nonspecific toxicities of Streptococcus pyogenes and Staphylococcus aureus dCas9 in Chlamydia trachomatis

2019 ◽  
Vol 77 (9) ◽  
Author(s):  
Wurihan Wurihan ◽  
Yehong Huang ◽  
Alec M Weber ◽  
Xiang Wu ◽  
Huizhou Fan
Keyword(s):  
Staphylococcus Aureus ◽  
Chlamydia Trachomatis ◽  
Streptococcus Pyogenes ◽  
Expression System ◽  
Knock Out ◽  
Guide Rna ◽  
Knock Down ◽  
Chromosomal Genes ◽  
Recent Advancement ◽  
And Staphylococcus Aureus

ABSTRACT Chlamydiae are common, important pathogens for humans and animals alike. Despite recent advancement in genetics, scientists are still searching for efficient tools to knock out or knock down the expression of chromosomal genes. We attempted to adopt a dCas9-based CRISPR interference (CRISPRi) technology to conditionally knock down gene expression in Chlamydia trachomatis using an anhydrotetracycline (ATC)-inducible expression system. Surprisingly, expression of the commonly used Streptococcus pyogenes dCas9 in C. trachomatis causes strong inhibition in the absence of any guide RNA (gRNA). Staphylococcus aureus dCas9 also shows strong toxicity in the presence of only an empty gRNA scaffold. Toxicity of the S. pyogenes dCas9 is readily observed with as little as 0.2 nM ATC. Growth inhibition by S. aureus dCas9 is evident starting at 1.0 nM ATC. In contrast, C. trachomatis growth was not affected by methionine-tRNA ligase overexpression induced with 10 nM ATC. We conclude that S. pyogenes and S. aureus dCas9 proteins in their current forms have limited utility for chlamydial research and suggest strategies to overcome this problem.

Expression of the Major Heat Shock Proteins DnaK and GroEL in Streptococcus pyogenes : A Comparison to Enterococcus faecalis and Staphylococcus aureus

2001 ◽  
Vol 42 (4) ◽  
pp. 264-268 ◽  
Author(s):  
Marinella S. Laport ◽  
Angela C.D. de Castro ◽  
Adriana Villardo ◽  
Jose Abelardo C. Lemos ◽  
Maria do Carmo F. Bastos ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Enterococcus Faecalis ◽  
Streptococcus Pyogenes ◽  
Shock Proteins ◽  
And Staphylococcus Aureus
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