scholarly journals Comparative evaluation of two different methods of inoculum preparation for antifungal susceptibility testing of filamentous fungi

2002 ◽  
Vol 50 (5) ◽  
pp. 719-722 ◽  
Author(s):  
A. Aberkane
Keyword(s):  
Filamentous Fungi ◽  
Comparative Evaluation ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Inoculum Preparation

scholarly journals Evaluation of ID Fungi Plates Medium for Identification of Molds by MALDI Biotyper

2020 ◽  
Vol 58 (5) ◽  
Author(s):  
Marie Gladys Robert ◽  
Charlotte Romero ◽  
Céline Dard ◽  
Cécile Garnaud ◽  
Odile Cognet ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Maldi Tof Ms ◽  
Extraction Protocol ◽  
Maldi Tof ◽  
Morphological Aspects ◽  
Routine Identification ◽  
Tof Ms

ABSTRACT MALDI-TOF mass spectrometry (MS) identification of pathogenic filamentous fungi is often impaired by difficulties in harvesting hyphae embedded in the medium and long extraction protocols. The ID Fungi Plate (IDFP) is a novel culture method developed to address such difficulties and improve the identification of filamentous fungi by MALDI-TOF MS. We cultured 64 strains and 11 clinical samples on IDFP, Sabouraud agar-chloramphenicol (SAB), and ChromID Candida agar (CAN2). We then compared the three media for growth, ease of harvest, amount of material picked, and MALDI-TOF identification scores after either rapid direct transfer (DT) or a long ethanol-acetonitrile (EA) extraction protocol. Antifungal susceptibility testing and microscopic morphology after subculture on SAB and IDFP were also compared for ten molds. Growth rates and morphological aspects were similar for the three media. With IDFP, harvesting of fungal material for the extraction procedure was rapid and easy in 92.4% of cases, whereas it was tedious on SAB or CAN2 in 65.2% and 80.3% of cases, respectively. The proportion of scores above 1.7 (defined as acceptable identification) were comparable for both extraction protocols using IDFP (P = 0.256). Moreover, rates of acceptable identification after DT performed on IDFP (93.9%) were significantly higher than those obtained after EA extraction with SAB (69.7%) or CAN2 (71.2%) (P = <0.001 and P = 0.001, respectively). Morphological aspects and antifungal susceptibility testing were similar between IDFP and SAB. IDFP is a culture plate that facilitates and improves the identification of filamentous fungi, allowing accurate routine identification of molds with MALDI-TOF-MS using a rapid-extraction protocol.

scholarly journals Ευαισθησίες των υφομυκήτων σε νεότερα αντιμυκητικά φάρμακα

2014 ◽  
Author(s):  
Ευαγγελία Πετρίκκου
Keyword(s):  
Filamentous Fungi ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Inoculum Size ◽  
Tween 20 ◽  
Second Phase ◽  
Registry Study ◽  
Broth Microdilution Method ◽  
Invasive Mycoses ◽  
Inoculum Preparation

The continuing increase of systemic and invasive mycoses due to filamentous fungi (moulds) such as species of Aspergillus, zygomycetes of the order Mucorales (Rhizopus, Mucor, θιπ.) and rare hyalohyphomycetes such as Fusarium and Scedosporium, and their difficult management because of their resistance in most antifungals, needed the development and standardization of a method to determine susceptibility to these drugs. The present dissertation aimed to the development of an antifungal susceptibility testing method which would be reliable and reproducible, in order to be used as a standard method. This method should be applicable to all filamentous fungi,The strains used for this study were isolates from cases with systemic mycoses with known antifungal susceptibility and outcome, as well as from a prospective registry study. The experiments of the first phase of the study aimed to verify the optimal conditions for the preparation of the inoculum of conidia to be tested for antifungal susceptibility with the broth microdilution method. The main conclusions of this phase were that the haemocytometer counting of conidia was a more reliable method of inoculum preparation than the spectrophotometric adjustment, and that Tween 20, a nonionic surfactant, should be used as a dispersing agent for the optimal suspension of hydrophobic conidia. The ideal inoculum size was 1.0 × 106 -5.0 × 106 CFU/ml. In the second phase, this new method was evaluated in three independent laboratories and its reliability and reproducibility were confirmed, with interlaboratory agreement of 89.2% and a narrow 95% CI (2.20 -2.65). In the third phase of the study the method was used for the susceptibility testing of strains from a prospective registry study of rare invasive mycoses and performed very well. The results of this study have been adopted by the EUCAST and constitute the basis of the guidelines for the determination of the MICs of antifungals against conidia forming moulds (EUCAST DEFINITIVE DOCUMENT E.DEF 9.1).

In vitro antifungal susceptibility testing of filamentous fungi with Sensititre Yeast OneTM

Mycoses ◽  
2006 ◽  
Vol 49 (4) ◽  
pp. 293-297 ◽  
Author(s):  
A. J. Carrillo-Munoz ◽  
G. Quindos ◽  
M. Ruesga ◽  
O. del Valle ◽  
J. Peman ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
In Vitro Antifungal Susceptibility

scholarly journals Comparative Evaluation of Etest, EUCAST, and CLSI Methods for Amphotericin B, Voriconazole, and Posaconazole against Clinically Relevant Fusarium Species

2016 ◽  
Vol 61 (1) ◽  
Author(s):  
Abdullah M. S. Al-Hatmi ◽  
Anne-Cécile Normand ◽  
Stephane Ranque ◽  
Renaud Piarroux ◽  
G. Sybren de Hoog ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Comparative Evaluation ◽  
Susceptibility Testing ◽  
Fusarium Species ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Content Type

ABSTRACT We compared EUCAST and CLSI methods versus Etest for antifungal susceptibility testing of 20 clinically relevant Fusarium species against amphotericin B, posaconazole, and voriconazole. The median Etest amphotericin B and posaconazole MICs were 1 dilution higher than the median EUCAST and the CLSI MICs. The essential agreement (within ±1/±2 dilutions) was 60/90%, 80/95%, and 70/85% between the Etest and EUCAST methods and 80/95%, 75/95%, and 45/100% between the Etest and CLSI methods for amphotericin B, voriconazole, and posaconazole, respectively. The categorical agreement was >85%. Etest can be used for antifungal susceptibility testing of Fusarium species.

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