scholarly journals Comparison of two multiplex immunochromatographic assays for the rapid detection of major carbapenemases in Enterobacterales

2020 ◽  
Vol 75 (6) ◽  
pp. 1491-1494 ◽  
Author(s):  
Pierre Bogaerts ◽  
Anne-Sophie Berger ◽  
Stéphanie Evrard ◽  
Te-Din Huang
Keyword(s):  
Rapid Detection ◽  
Reference Method ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Mean Intensity ◽  
Reference Center ◽  
National Reference ◽  
The Mean ◽  
Imagej Software ◽  
National Reference Center

Abstract Objectives Two commercially available lateral flow immunochromatographic assays (ICAs) for detection of the major carbapenemases were prospectively assessed for the detection of carbapenemases in Enterobacterales: RESIST-4 O.K.N.V. (Coris BioConcept) and NG-Test CARBA 5 (NG Biotech). Methods These two assays were performed prospectively on consecutive Enterobacterales suspected of producing a carbapenemase that were referred to the Belgian National Reference Center for Monitoring Antimicrobial Resistance in Gram-Negative Bacteria between March and June 2018. The intensity of the band corresponding to a carbapenemase for each test was compared using ImageJ software. Results Of the 161 isolates tested, a carbapenemase was detected in 91 (60 OXA-48-like, 15 VIM, 9 KPC, 5 NDM, 1 IMP and 1 IMP + OXA-48); in the remaining 70, no carbapenemases were detected. For both tests, the results were 100% concordant with the results of the PCR-sequencing reference method. Two IMP producers were only detected by NG-Test CARBA 5 as IMP is not targeted by RESIST-4 O.K.N.V. The mean intensity of the OXA-48, VIM and NDM bands displayed by NG-Test CARBA 5 was 3 to 3.7 times higher than for RESIST-4 O.K.N.V., while the KPC band was on average 1.7 times more intense with RESIST-4 O.K.N.V. Conclusions RESIST-4 O.K.N.V. and NG-Test CARBA 5 are two efficient assays for identification of the major carbapenemases. NG-Test CARBA 5 offers the advantage of detecting IMP, which remains rare in Western countries.

scholarly journals Multicenter Evaluation of a New DNA Microarray for Rapid Detection of Clinically RelevantblaGenes from β-Lactam-Resistant Gram-Negative Bacteria

2011 ◽  
Vol 55 (9) ◽  
pp. 4457-4460 ◽  
Author(s):  
Pierre Bogaerts ◽  
Andrea M. Hujer ◽  
Thierry Naas ◽  
Roberta Rezende de Castro ◽  
Andrea Endimiani ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Dna Microarray ◽  
Rapid Detection ◽  
Gene Sequencing ◽  
Reference Method ◽  
Low Density ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Content Type ◽  
Reference Collection

ABSTRACTA new commercial low-density microarray which identifies common extended-spectrum β-lactamase plasmid-mediated cephalosporinase genes, as well as carbapenemase (blaKPCandblaNDM) genes, was evaluated. We tested 207 clinical and reference/collection isolates of theEnterobacteriaceaepossessing differentblagenes. Overall, the sensitivity and specificity of the microarray were 100% for the detection of the plasmid-mediatedblaAmpC,blaKPC, andblaNDMgenes usingblagene sequencing as the reference method.

Identification and Drug Sensitivity of Aerobic Bacterial Isolates from Diabetic Foot Ulcers of Sudanese Patients: a Cross sectional study

2020 ◽  
Author(s):  
saada Mohamed nour ◽  
Maowia Mukhtar ◽  
Mohamed Elmakk ◽  
Elshibli Mohamed Elshibli ◽  
Walyeldin Elnour Elfakey ◽  
...  
Keyword(s):  
Diabetic Foot ◽  
Diffusion Method ◽  
Foot Ulcers ◽  
Aerobic Bacteria ◽  
Diabetic Patients ◽  
Gram Negative Bacteria ◽  
Female Ratio ◽  
Gram Negative ◽  
The Mean ◽  
Grade 3

Abstract Background Diabetic foot ulcer infection cause great morbidity and mortality among diabetic patients and is a major cause of lower extremity amputation worldwide. This study aimed to determine the profile of aerobic bacteria and their antibiotic sensitivity patterns in diabetic foot infections (DFI) among different Wagner's grades. Methods This study was conducted during December 2017 - March 2018 in a Diabetic Center, Sudan. A total of 152 diabetic patients with different grades of foot ulcers were randomly enrolled in the study. The patients were grouped using Wagner's classification. Tissue biopsies and deep swabs were collected from the ulcers for aerobic cultures. The cultured isolates were identified using phenotypic and biochemical properties and their sensitivity to commonly used antibiotics, Colistin, Aikacin, Ciprofloxacin, Augmentin, Ceftazideme, Gentamicin, Clindamycin, Ceftriaxone Meropenum. Cotrimoxazole, Erythomycin, Oxacillin and Vancomycin. Fusidic acid, Imepenem, and Piperacillin was tested using the Kirby Bauer disk diffusion method. Results The mean age of the patients was 54.31 (SD ± 12.1) years, male to female ratio of 8: 1. The mean duration of diabetes was 14 (SD ± 8) years. The ulcers varied in duration from 1 day to 10 years. of 152 samples 181 aerobic bacteria were isolated. Cultures yielded 1-3 isolate per culture. The maximum number was isolated from grade 3 group followed by long standing ulcer LSU group 50.8% and 28% respectively. Polymicrobial infection was higher in LSU (30.4%). The isolates were mostly Gram-negative bacteria. The most frequent were proteus spp. (35.3%), S. aureus MRSA 14.4% and Coliform 12.2% respectively. The most common isolates in grade 3 were P. Mirablis, Staphylococcus and Coliform and in long standing ulcers were P. Mirablis, S. aureus MRSA and Coagulase negative staphylococcus respectively. Conclusion Gram-negative bacteria were more prevalent and the most frequent pathogens were Proteus spp. The most common polymicrobial infections were due to P. mirablis with; P. aerginosa, S. MRSA and Coliform respectively . Gram negative rods were sensitive to Amikacin, (80.6) %) while the highest sensitivity of Gram positive was to Imepnem (85%). Most of the isolates were sensitive to Meropenem. No significant relation between Wagner grades and neuropathy was detected.

scholarly journals Trends in carbapenemase-producing Enterobacteriaceae, France, 2012 to 2014

2017 ◽  
Vol 22 (6) ◽  
Author(s):  
Laurent Dortet ◽  
Gaëlle Cuzon ◽  
Valérie Ponties ◽  
Patrice Nordmann
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Mechanism ◽  
Tropical Region ◽  
Reference Center ◽  
National Reference ◽  
National Reference Center

In 2014, a total of 2,976 Enterobacteriaceae isolates with decreased susceptibility to carbapenems were received at the French Associated National Reference Center for Antibiotic Resistance (NRC) and were characterised for their molecular resistance mechanism to carbapenems and compared with results obtained during 2012 and 2013.The overall number of enterobacterial isolates with decreased susceptibility to carbapenems received at the NRC rapidly increased (more than twofold in two years) with a growing proportion of carbapenemase producers (23.1% in 2012 vs 28.6% in 2013 vs 36.2% in 2014). Between 2012 and 2014, the main carbapenemase type was OXA-48, with an increase in OXA-48 variants (mostly OXA-181) and NDM producers, whereas the number KPC producers decreased. We identified a potential spread of OXA-181 producers in the tropical region of Africa. Finally, OXA-48 and OXA-48-related enzymes remained the predominant carbapenemases in France. The number of carbapenemase-producing Escherischia coli isolates was multiplied by fivefold between 2012 and 2014, suggesting a possible dissemination in the community.

scholarly journals Clinical Features and Complications ofCoxiella burnetiiInfections From the French National Reference Center for Q Fever

2018 ◽  
Vol 1 (4) ◽  
pp. e181580 ◽  
Author(s):  
Cléa Melenotte ◽  
Camélia Protopopescu ◽  
Matthieu Million ◽  
Sophie Edouard ◽  
M. Patrizia Carrieri ◽  
...  
Keyword(s):  
Clinical Features ◽  
Q Fever ◽  
Reference Center ◽  
National Reference ◽  
National Reference Center

Spinal cord injury in pediatric age in Spain. Reality of a national reference center

2015 ◽  
Vol 31 (6) ◽  
pp. 917-921 ◽  
Author(s):  
Sagrario Pérez- de la Cruz ◽  
Veronica Cimolin ◽  
Angel Gil-Agudo
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Pediatric Age ◽  
Reference Center ◽  
National Reference ◽  
Cord Injury ◽  
National Reference Center
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