scholarly journals In vitro antimicrobial susceptibility of clinical isolates of Aeromonas caviae, Aeromonas hydrophila and Aeromonas veronii biotype sobria

2002 ◽  
Vol 49 (4) ◽  
pp. 701-702 ◽  
Author(s):  
J. Vila ◽  
F. Marco ◽  
L. Soler ◽  
M. Chacon ◽  
M. J. Figueras
Keyword(s):  
Antimicrobial Susceptibility ◽  
Aeromonas Hydrophila ◽  
Clinical Isolates ◽  
Aeromonas Veronii ◽  
Aeromonas Caviae

scholarly journals In vitro antimicrobial susceptibility in clinical isolates of Enterococcus species

2003 ◽  
Vol 45 (2) ◽  
Author(s):  
Ernesto Calderón-Jaimes ◽  
José Luis Arredondo-García ◽  
Felipe Aguilar-Ituarte ◽  
Pilar García-Roca
Keyword(s):  
Antimicrobial Susceptibility ◽  
Clinical Isolates ◽  
Enterococcus Species

In vitro antimicrobial susceptibility of equine clinical isolates from France, 2006–2016

2019 ◽  
Vol 19 ◽  
pp. 144-153 ◽  
Author(s):  
Rachel Duchesne ◽  
Sophie Castagnet ◽  
Karine Maillard ◽  
Sandrine Petry ◽  
Vincent Cattoir ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Clinical Isolates

Phenotypic identification ofAeromonasgenomospecies from clinical and environmental sources

1998 ◽  
Vol 44 (2) ◽  
pp. 103-108 ◽  
Author(s):  
N Borrell ◽  
M J Figueras ◽  
J Guarro
Keyword(s):  
Drinking Water ◽  
Aeromonas Hydrophila ◽  
Sea Water ◽  
Aeromonas Veronii ◽  
Aeromonas Caviae ◽  
Food Borne ◽  
Lakes And Reservoirs ◽  
Treated Drinking Water ◽  
Prevalent Species ◽  
Isolated Species

A collection of 983 Aeromonas isolates from environmental and clinical sources have been identified to the genomospecies level. A phenotypic method identified 93% of the strains. The use of citrate and the production of acid from sorbitol enabled the members of the Aeromonas hydrophila complex to be separated. The most common genomospecies from intestinal sources were Aeromonas veronii biotype sobria and Aeromonas caviae. The former, together with A. hydrophila,was the most frequently isolated species of extraintestinal origin. Most pathogenic species were very prevalent in environmental samples, with A. veronii biotype sobria being the most common in lakes and reservoirs (41.5%) and in treated drinking water (25.0%), and A. caviae was the most common in sea water (26.0%) and milk products (35.5%). Aeromonas hydrophila (18.1%) was the second most prevalent species isolated in untreated drinking water. Since Aeromonas infections are generally regarded as a water- and food-borne diseases, the high environmental prevalence of these pathogenic genomospecies should be regarded as an important threat to public health.Key words: Aeromonas, food, water, clinical, Spain.

scholarly journals Feasibility and potential significance of rapid in vitro qualitative phenotypic antimicrobial susceptibility testing of gram-negative bacilli with the ProMax system

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0249203
Author(s):  
Jade Chen ◽  
Michael Tomasek ◽  
Amorina Cruz ◽  
Matthew L. Faron ◽  
Dakai Liu ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Pathogenic Bacteria ◽  
Susceptibility Testing ◽  
Klebsiella Oxytoca ◽  
Antimicrobial Susceptibility Testing ◽  
Clinical Isolates ◽  
Clinical Samples ◽  
Automation System ◽  
Gram Negative

The emergence and evolution of antibiotic resistance has been accelerated due to the widespread use of antibiotics and a lack of timely diagnostic tests that guide therapeutic treatment with adequate sensitivity, specificity, and antimicrobial susceptibility testing (AST) accuracy. Automated AST instruments are extensively used in clinical microbiology labs and provide a streamlined workflow, simplifying susceptibility testing for pathogenic bacteria isolated from clinical samples. Although currently used commercial systems such as the Vitek2 and BD Phoenix can deliver results in substantially less time than conventional methods, their dependence on traditional AST inoculum concentrations and optical detection limit their speed somewhat. Herein, we describe the GeneFluidics ProMax lab automation system intended for a rapid 3.5-hour molecular AST from clinical isolates. The detection method described utilizes a higher starting inoculum concentration and automated molecular quantification of species-specific 16S rRNA through the use of an electrochemical sensor to assess microbiological responses to antibiotic exposure. A panel of clinical isolates consisting of species of gram-negative rods from the CDC AR bank and two hospitals, New York-Presbyterian Queens and Medical College of Wisconsin, were evaluated against ciprofloxacin, gentamicin, and meropenem in a series of reproducibility and clinical studies. The categorical agreement and reproducibility for Citrobacter freundii, Enterobacter cloacae, Escherichia coli, Klebsiella aerogenes, Klebsiella oxytoca, Klebsiella pneumoniae, and Pseudomonas aeruginosa were 100% and 100% for ciprofloxacin, 98.7% and 100% for gentamicin and 98.5% and 98.5% for meropenem, respectively.

scholarly journals SELEKSI KANDIDAT PROBIOTIK ANTI AEROMONAS HYDROPHILA UNTUK PENGENDALIAN PENYAKIT IKAN AIR TAWAR

2012 ◽  
Vol 7 (2) ◽  
pp. 269
Author(s):  
Angela Mariana Lusiastuti
Keyword(s):  
Aeromonas Hydrophila ◽  
Alcaligenes Faecalis ◽  
Bacillus Firmus ◽  
Aeromonas Caviae

Secara dasar ada tiga model kerja probiotik yaitu menekan populasi mikroba melalui kompetisi dengan memproduksi senyawa anti-mikroba atau melalui kompetisi nutrisi dan tempat pelekatan di dinding usus, mengubah keseimbangan metabolisme mikroba dengan meningkatkan atau menurunkan aktivitas enzim dan menstimulasi imunitas dengan meningkatkan antibodi dan aktivitas makrofag. Tujuan penelitian ini adalah memperoleh mikroba yang berpotensi sebagai kandidat probiotik untuk dapat diaplikasikan di dalam menekan atau menghambat bakteri patogen sebagai langkah pengendalian penyakit pada ikan air tawar. Penelitian ini terdiri atas dua tahap, tahap pertama adalah tahap seleksi yang dimulai dari koleksi dan isolasi bakteri kandidat probiotik dan tahap kedua adalah tahap pengujian bakteri kandidat probiotik secara in vitro menggunakan metode daya hambat dan menguji daya patogenisitas bakteri. Total isolat bakteri yang diperoleh adalah 72 isolat, dan hanya enam isolat terpilih sebagai kandidat probiotik anti Aeromonas hydrophila. Probiotik anti A. hydrophila adalah sebagai berikut: Chromobacterium lividum, Alcaligenes faecalis, Aeromonas caviae, Bacteriodes serpens, Bacillus firmus, Aeromonas caviae = A. hydrophila Sub sp. Anaerogenes.

scholarly journals In Vitro Antimicrobial Susceptibility of Clinical Isolates of Borrelia miyamotoi

2018 ◽  
Vol 62 (7) ◽  
pp. e00419-18 ◽  
Author(s):  
Joris Koetsveld ◽  
Annemijn Manger ◽  
Dieuwertje Hoornstra ◽  
Ronald O. Draga ◽  
Anneke Oei ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Dark Field ◽  
Clinical Isolates ◽  
Borrelia Miyamotoi ◽  
Content Type ◽  
Dark Field Microscopy ◽  
Made In

ABSTRACT Borrelia miyamotoi is an emerging relapsing fever (RF) Borrelia species that is reported to cause human disease in regions in which Lyme borreliosis is endemic. We recently showed that B. miyamotoi tick isolates are resistant to amoxicillin in vitro; however, clinical isolates have not been studied. Therefore, our aim was to show the antimicrobial susceptibility of recently obtained clinical isolates of B. miyamotoi. A dilution series of various antibiotics was made in modified Kelly-Pettenkofer medium with 10% fetal calf serum. The susceptibilities of different B. miyamotoi clinical, B. miyamotoi tick, RF Borrelia, and Borrelia burgdorferi sensu lato isolates were tested by measuring MICs through colorimetric changes and by counting motile spirochetes by dark-field microscopy after 72 h of incubation. The ceftriaxone and azithromycin MIC ranges of the six B. miyamotoi clinical isolates tested were 0.03 to 0.06 mg/liter and 0.0016 to 0.0032 mg/liter, respectively. These values are similar to MICs for RF Borrelia strains and B. miyamotoi tick isolates. All tested RF Borrelia strains were susceptible to doxycycline (microscopic MIC range, 0.0625 to 0.25 mg/liter). In contrast to the MICs of the tested B. burgdorferi sensu lato strains and in line with our previous findings, the amoxicillin MICs (range, 8 to 32 mg/liter) of all RF Borrelia strains, including B. miyamotoi clinical isolates, were above the clinical breakpoint for resistance (≤4 mg/liter). Clinical isolates of B. miyamotoi are highly susceptible to doxycycline, azithromycin, and ceftriaxone in vitro. Interestingly, as described previously for tick isolates, amoxicillin shows poor in vitro activity against B. miyamotoi clinical isolates.

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