scholarly journals Patterns and mechanisms of resistance to beta-lactams and beta-lactamase inhibitors in uropathogenic Escherichia coli isolated from dogs in Portugal

2002 ◽  
Vol 49 (1) ◽  
pp. 77-85 ◽  
Author(s):  
C. Feria
Keyword(s):  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli ◽  
Beta Lactamase ◽  
Beta Lactams ◽  
Mechanisms Of Resistance

scholarly journals Nosocomial spread of cephem-resistant Escherichia coli strains carrying multiple Toho-1-like beta-lactamase genes.

1997 ◽  
Vol 41 (12) ◽  
pp. 2606-2611 ◽  
Author(s):  
T Yagi ◽  
H Kurokawa ◽  
K Senda ◽  
S Ichiyama ◽  
H Ito ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Southern Hybridization ◽  
Restriction Analysis ◽  
Beta Lactamase ◽  
Amino Acid Residues ◽  
Chromosomal Dna ◽  
Beta Lactams ◽  
E Coli ◽  
Similar Genetic Background ◽  
Different Origins

Escherichia coli HKY56, which demonstrated resistance to various beta-lactams except carbapenems, was isolated from the throat swab of an inpatient in 1994. Conjugal transfer of cephem resistance from HKY56 to E. coli CSH2 was not successful. Three cefotaxime-resistant E. coli clones harboring plasmid pMRE001, pMRE002, or pMRE003, each of which carried a 3.4-, 5.8-, or 6.2-kb EcoRI fragment insert, respectively, were obtained from HKY56. Although restriction analysis suggested their different origins, these clones showed similar profiles of resistance to various beta-lactams. The sequence of 10 amino acid residues at the N terminus of beta-lactamase purified from E. coli HB101(pMRE001) was identical to that of Toho-1. This Toho-1-like beta-lactamase-1 (TLB-1) was able to hydrolyze cefoperazone and cefotaxime efficiently, but it failed to hydrolyze cephamycins. A Toho-1-specific DNA probe was hybridized with three distinct EcoRI fragments derived from the chromosomal DNA of strain HKY56, and these fragments corresponded to DNA inserts carried by pMRE001, pMRE002, and pMRE003, respectively. PCR and Southern hybridization analysis suggested that all six cephem-resistant E. coli strains, strains HKY273, HKY285, HKY288, HKY305, HKY316, and HKY335, which were isolated in 1996 at the same hospital where strain HKY56 had been isolated, also possessed multiple Toho-1-like beta-lactamase (TLB) genes, and the hybridization patterns obtained with the Toho-1-specific probe were quite similar among these six isolates. The DNA fingerprinting patterns observed by pulsed-field gel electrophoresis revealed that among the E. coli isolates tested, all isolates except HKY56 possessed a similar genetic background. These findings suggested that E. coli strains that carry chromosomally multiplied TLB genes may have been proliferating and transmitted among patients in the same hospital.

Biofilm and Extended Spectrum Beta Lactamase Production amongst Uropathogenic Escherichia Coli Isolates at the University of Ilorin Teaching Hospital, Nigeria

2019 ◽  
Author(s):  
Dele Ohinoyi Amadu ◽  
Charles Nwabuisi ◽  
Yahaya Usman ◽  
Jelili Olaide Mustapha ◽  
Idris Nasir Abdullahi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Teaching Hospital ◽  
Uropathogenic Escherichia Coli ◽  
Health Concern ◽  
Beta Lactamase ◽  
Antibacterial Resistance ◽  
Esbl Production ◽  
Isolation And Identification ◽  
Biofilm Production ◽  
The University

Background and Aims: Uropathogenic Escherichia coli (UPEC) are considered major reservoir for genes encoding antimicrobial resistance. The mechanism of resistance and persistence of UPEC has been attributed to the production of biofilm and Extended Beta Lactamase (ESBL). This hospital-based prospective study determined how biofilm and ESBL production facilitate antibacterial resistance amongst UPEC isolated from catheter urine of patients attending the University of Ilorin Teaching Hospital, Nigeria.  Materials and Methods: Urine samples from 113 catheterized inpatients and outpatients were analysed. Female subjects accounted for 47 (41.6%) of the study population. Standard microbiological methods and Analytical Profile Index (API) 20E were used for the isolation and identification of UPEC. Tissue culture plate technique was used to demonstrate biofilm production potentials and double-disc synergy test was used to determine ESBL production. Results: Catheter associated urinary tract infection in this study was 70.8% of samples analysed. Of this, Escherichia coli, 44 (55.0%) was the most predominant. UPEC, biofilm and ESBL production amounted to 38.9%, 81.8% and 27.2%, respectively. ESBL production was significantly associated with degree of biofilm formation (p<0.005). Both strong and moderate biofilm producers showed the same level of resistance to ceftazidime (31.6%). Moderate biofilm producers were 46.7% resistant to cefriaxone. Resistance to Amoxillin-clauvanate significantly occurred in all grades of biofilm producers (p>0.05). Imipenem, however, was the most sensitive with no resistance by the UPEC.  Conclusions: ESBL and biofilm production were associated with antibacterial resistance. The incidence of ESBL production amongst biofilm forming UPEC is of great public health concern.

Occurrence of beta-lactamase producing Escherichia coli in healthy and diarrhoeic dogs in Andhra Pradesh, India

2018 ◽  
Author(s):  
N. Mohammad Sharif ◽  
B. Sreedevi ◽  
R. K. Chaitanya ◽  
Ch. Srilatha
Keyword(s):  
Escherichia Coli ◽  
Andhra Pradesh ◽  
Diffusion Method ◽  
Beta Lactamase ◽  
Beta Lactam ◽  
Beta Lactams ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Healthy Dogs ◽  
Group 2

The present study was carried out to characterize beta-lactam resistance in Escherichia coli isolated from healthy and diarrhoeic dogs. A total of 93 E. coli were isolated from the rectal swabs of 136 dogs (60/92 of healthy dogs and 33/44 of diarrhoeic dogs). Predominant serotypes detected include rough (19 isolates), O141 (5), O9 (2), O126 (2), O128 (2), O15, O20, O35, O49, O63, O85, O101, O116, O117, O118, O119 (1 isolate each) and the rest of 52 isolates were untypable (UT). Disc diffusion method revealed resistance to cefotaxime (41.9%), ceftriaxone (34.4%), ceftazidime (30.1%) and aztreonam (18.2%). Overall frequency of extended spectrum beta-lactamase (ESBL) phenotype was found to be 29% (27/93). Beta-lactamase genes detected include blaAmpC (86.0%), blaSHV (30.1%), blaCTX-M group-1 (19.3%), blaTEM (17.2%), blaOXA (13.9%) and blaCTX-M group-2 (7.5%). The study revealed resistance to commonly prescribed beta-lactams, with ESBL phenotype in E. coli of canine origin in Andhra Pradesh, India.

scholarly journals Biofilm production in relation to extended spectrum beta-lactamase production and antibiotic resistance among uropathogenic Escherichia coli

2017 ◽  
Vol 5 (1) ◽  
pp. 61-63
Author(s):  
Samip Khatri ◽  
Narayan Dutt Pant ◽  
Sanjeev Neupane ◽  
Suman Bhandari ◽  
Megha Raj Banjara
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Uropathogenic Escherichia Coli ◽  
Beta Lactamase ◽  
Medical Sciences ◽  
Extended Spectrum Beta Lactamase ◽  
Medical College ◽  
Biofilm Production ◽  
Extended Spectrum

Not available. Janaki Medical College Journal of Medical Sciences (2017) Vol. 5(1): 61-63

scholarly journals CTX-M-32 beta-lactamase-producing uropathogenic Escherichia coli isolated in Latin America

2013 ◽  
Vol 7 (05) ◽  
pp. 432-435 ◽  
Author(s):  
María Araque ◽  
Beatriz Millán ◽  
Evelin Mendoza ◽  
Marianella Salas
Keyword(s):  
Escherichia Coli ◽  
Latin America ◽  
Full Text ◽  
Uropathogenic Escherichia Coli ◽  
Beta Lactamase

This item has no abstract. Follow the links below to access the full text.

A novel class C beta-lactamase (FOX-2) in Escherichia coli conferring resistance to cephamycins.

1997 ◽  
Vol 41 (9) ◽  
pp. 2041-2046 ◽  
Author(s):  
A Bauernfeind ◽  
S Wagner ◽  
R Jungwirth ◽  
I Schneider ◽  
D Meyer
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Amino Acid ◽  
Tract Infection ◽  
Coli Strain ◽  
Beta Lactamase ◽  
Beta Lactams ◽  
Beta Lactamases ◽  
Pcr Product

An Escherichia coli strain resistant to a broad spectrum of beta-lactams, including cephamycins, was isolated from a patient suffering from urinary tract infection. A resistance plasmid (pMVP-7) was transferred from the clinical isolate to an Escherichia coli recipient. Both strains produce a cefoxitin-hydrolyzing beta-lactamase focusing at pI 6.7. The phenotype was similar to that of a Klebsiella pneumoniae strain producing cephamycinase FOX-1, so primers were selected from the FOX-1 sequence to amplify the bla gene of the transconjugant. The PCR product obtained was sequenced. The percentage of identity of the deduced amino acid sequence with sequences of other AmpC-type beta-lactamases was 96.9% with FOX-1, 74.9% with CMY-1, and 67.7% with MOX-1. This new plasmid-mediated enzyme is most closely related to FOX-1 (11 amino acid exchanges). We therefore propose the designation FOX-2.

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