scholarly journals Multi-centre evaluation of the Etest method for antifungal drug susceptibility testing of Candida spp. and Cryptococcus neoformans. BSAC Working Party on Antifungal Chemotherapy.

1998 ◽  
Vol 42 (3) ◽  
pp. 321-331 ◽  
Author(s):  
D. W. Warnock ◽  
E. M. Johnson ◽  
T. R. Rogers
Keyword(s):  
Cryptococcus Neoformans ◽  
Susceptibility Testing ◽  
Working Party ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Antifungal Drug ◽  
Candida Spp ◽  
Antifungal Chemotherapy

Antifungal drug susceptibility testing and resistance in Aspergillus

1999 ◽  
Vol 2 (5) ◽  
pp. 326-334 ◽  
Author(s):  
David W. Warnock ◽  
Beth A. Arthington-Skaggs ◽  
Ren-Kai Li
Keyword(s):  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Antifungal Drug

scholarly journals Clinical implications of antifungal drug susceptibility testing of dermatophytes

2019 ◽  
Vol 10 (6) ◽  
pp. 737
Author(s):  
Ananta Khurana ◽  
Kabir Sardana ◽  
Anuradha Chowdhary ◽  
Khushboo Sethia
Keyword(s):  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Antifungal Drug ◽  
Clinical Implications

scholarly journals Comparative analysis of Etest and broth microdilution method (AFST-EUCAST) for trends in antifungal drug susceptibility testing of Brazilian Cryptococcus neoformans isolates

2006 ◽  
Vol 55 (12) ◽  
pp. 1693-1699 ◽  
Author(s):  
Amanda L. T. Dias ◽  
Flavia E. Matsumoto ◽  
Marcia S. C. Melhem ◽  
Eriques G. da Silva ◽  
Marcos E. Auler ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Amphotericin B ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Correlation Coefficients ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Microdilution Method ◽  
Broth Microdilution Method

A prospective study was performed to evaluate the correlation between the proposed standard of the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antibiotic Susceptibility Testing (AFST-EUCAST) (document 7.1) and the commercial system Etest for determining the MICs of flucytosine, amphotericin B, fluconazole, itraconazole and voriconazole for a collection of 100 clinical and environmental isolates of Cryptococcus neoformans. The agreements among Etest MICs within ±2 log2 dilutions of AFST-EUCAST standard MICs were greater for flucytosine, fluconazole and voriconazole (76, 78 and 88 %, respectively) than for amphotericin B (5 %), the lowest agreement, and itraconazole (67 %). Overall, the correlation coefficients were statistically significant (P<0.05), and it is suggested that the Etest and AFST-EUCAST method are reliable alternatives and present good correlation for all drugs evaluated except amphotericin B. However, the observed differences related to MICs for susceptible, susceptible dose dependent and resistant strains between the methods suggest that it will be necessary to carry out further studies, including assessment of interlaboratory agreement and correlation of MICs by different methods with in vivo response.

Antifungal drug susceptibility testing

1994 ◽  
Vol 32 (s1) ◽  
pp. 267-276 ◽  
Author(s):  
J. Van Cutsem ◽  
H. Kurata ◽  
H. Matsuoka ◽  
Y. Mikami ◽  
M.A. Pfaller ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Antifungal Drug

scholarly journals Comparative Evaluation of FUNGITEST and Broth Microdilution Methods for Antifungal Drug Susceptibility Testing of Candida Species and Cryptococcus neoformans

1998 ◽  
Vol 36 (4) ◽  
pp. 926-930 ◽  
Author(s):  
Kate G. Davey ◽  
Ann D. Holmes ◽  
Elizabeth M. Johnson ◽  
Adrien Szekely ◽  
David W. Warnock
Keyword(s):  
Cryptococcus Neoformans ◽  
Amphotericin B ◽  
Susceptibility Testing ◽  
Clinical Laboratory ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
National Committee ◽  
Broth Microdilution ◽  
Fluconazole Resistant

The FUNGITEST method (Sanofi Diagnostics Pasteur, Paris, France) is a microplate-based procedure for the breakpoint testing of six antifungal agents (amphotericin B, flucytosine, fluconazole, itraconazole, ketoconazole, and miconazole). We compared the FUNGITEST method with a broth microdilution test, performed according to National Committee for Clinical Laboratory Standards document M27-A guidelines, for determining the in vitro susceptibilities of 180 isolates ofCandida spp. (50 C. albicans, 50C. glabrata, 10 C. kefyr, 20C. krusei, 10 C. lusitaniae, 20C. parapsilosis, and 20 C. tropicalisisolates) and 20 isolates of Cryptococcus neoformans. Overall, there was 100% agreement between the methods for amphotericin B, 95% agreement for flucytosine, 84% agreement for miconazole, 83% agreement for itraconazole, 77% agreement for ketoconazole, and 76% agreement for fluconazole. The overall agreement between the methods exceeded 80% for all species tested with the exception ofC. glabrata (71% agreement). The poorest agreement between the results for individual agents was seen with C. glabrata (38% for fluconazole, 44% for ketoconazole, and 56% for itraconazole) and C. tropicalis (50% for miconazole). The FUNGITEST method misclassified as susceptible 2 of 12 (16.6%) fluconazole-resistant isolates, 2 of 10 (20%) itraconazole-resistant isolates, and 4 of 8 (50%) ketoconazole-resistant isolates of several Candida spp. Further development of the FUNGITEST procedure will be required before it can be recommended as an alternative method for the susceptibility testing of Candida spp. or C. neoformans.

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