scholarly journals High frequency of mutations at position 2144 of the 23S rRNA gene in clarithromycin-resistant Helicobacter pylori strains isolated in Spain

1998 ◽  
Vol 41 (5) ◽  
pp. 573-574 ◽  
Author(s):  
D Domingo
Keyword(s):  
Helicobacter Pylori ◽  
High Frequency ◽  
23S Rrna ◽  
Rrna Gene ◽  
23S Rrna Gene

Identification of a 23S rRNA Gene Mutation in Clarithromycin-Resistant Helicobacter pylori

Helicobacter ◽  
1996 ◽  
Vol 1 (4) ◽  
pp. 227-228 ◽  
Author(s):  
Gregory G. Stone ◽  
Dee Shortridge ◽  
Robert K. Flamm ◽  
James Versalovic ◽  
Jill Beyer ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gene Mutation ◽  
23S Rrna ◽  
Rrna Gene ◽  
23S Rrna Gene

scholarly journals Genotypic Characterization of Clarithromycin-Resistant and -Susceptible Helicobacter pylori Strains from the Same Patient Demonstrates Existence of Two Unrelated Isolates

1998 ◽  
Vol 36 (9) ◽  
pp. 2730-2731 ◽  
Author(s):  
Ge Wang ◽  
Qin Jiang ◽  
Diane E. Taylor
Keyword(s):  
Helicobacter Pylori ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Pulsed Field Gel Electrophoresis ◽  
23S Rrna ◽  
Rrna Gene ◽  
Clarithromycin Resistance ◽  
23S Rrna Gene ◽  
Mode Of Development

Clarithromycin-susceptible and clarithromycin-resistantHelicobacter pylori isolates from the same patient were investigated for the mode of development and mechanism of clarithromycin resistance. The clarithromycin-resistant strain UA1182 harbors homozygous A-to-G mutations at position 2143 in both copies of the 23S rRNA gene and has a phenotype of resistance to clarithromycin and clindamycin but no significant resistance to streptogramin B. Pulsed-field gel electrophoresis patterns of NruI- andNotI-digested genomic DNA from the Clas and Clar isolates demonstrated that they are genetically distinct, suggesting that the development of clarithromycin resistance is not from the mutation of the existing Clas strain but from a completely new strain.

scholarly journals Mutations in the 23S rRNA gene are associated with clarithromycin resistance in Helicobacter pylori isolates in Brazil

2003 ◽  
Vol 2 (1) ◽  
Author(s):  
Marcelo L Ribeiro ◽  
Lea Vitiello ◽  
Maira CB Miranda ◽  
Yune HB Benvengo ◽  
Anita PO Godoy ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
23S Rrna ◽  
Rrna Gene ◽  
Clarithromycin Resistance ◽  
23S Rrna Gene

Low prevalence of clarithromycin-resistant Helicobacter pylori isolates with A2143G point mutation in the 23S rRNA gene in North India

2016 ◽  
Vol 6 ◽  
pp. 39-43 ◽  
Author(s):  
Valentina Gehlot ◽  
Shweta Mahant ◽  
Asish Kumar Mukhopadhyay ◽  
Kunal Das ◽  
Jawed Alam ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Point Mutation ◽  
North India ◽  
23S Rrna ◽  
Rrna Gene ◽  
23S Rrna Gene ◽  
Low Prevalence

Mutations in the 23S rRNA gene of clarithromycin-resistant Helicobacter pylori from Japan

2007 ◽  
Vol 30 (3) ◽  
pp. 250-254 ◽  
Author(s):  
Emiko Rimbara ◽  
Norihisa Noguchi ◽  
Hidenobu Kijima ◽  
Tai Yamaguchi ◽  
Takashi Kawai ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
23S Rrna ◽  
Rrna Gene ◽  
23S Rrna Gene

scholarly journals New Site of Modification of 23S rRNA Associated with Clarithromycin Resistance of Helicobacter pylori Clinical Isolates

2002 ◽  
Vol 46 (12) ◽  
pp. 3765-3769 ◽  
Author(s):  
Carla Fontana ◽  
Marco Favaro ◽  
Silvia Minelli ◽  
Anna Angela Criscuolo ◽  
Antonio Pietroiusti ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
23S Rrna ◽  
Dilution Method ◽  
Rrna Gene ◽  
Functional Sites ◽  
Biopsy Specimens ◽  
Clarithromycin Resistance ◽  
Pcr Product ◽  
23S Rrna Gene ◽  
H Pylori

ABSTRACT Resistance of Helicobacter pylori to clarithromycin occurs with a prevalence ranging from 0 to 15%. This has an important clinical impact on dual and triple therapies, in which clarithromycin seems to be the better choice to achieve H. pylori eradication. In order to evaluate the possibility of new mechanisms of clarithromycin resistance, a PCR assay that amplified a portion of 23S rRNA from H. pylori isolates was used. Gastric tissue biopsy specimens from 230 consecutive patients were cultured for H. pylori isolation. Eighty-six gastric biopsy specimens yielded H. pylori-positive results, and among these 12 isolates were clarithromycin resistant. The latter were studied to detect mutations in the 23S rRNA gene. Sequence analysis of the 1,143-bp PCR product (portion of the 23S rRNA gene) did not reveal mutation such as that described at position 2142 to 2143. On the contrary, our findings show, for seven isolates, a T-to-C transition at position 2717. This mutation conferred a low level of resistance, equivalent to the MIC for the isolates, selected using the E-test as well as using the agar dilution method: 1 μg/ml. Moreover, T2717C transition is located in a highly conserved region of the 23S RNA associated with functional sites: domain VI. This fact has a strong effect on the secondary structure of the 23S RNA and on its interaction with macrolide. Mutation at position 2717 also generated an HhaI restriction site; therefore, restriction analysis of the PCR product also permits a rapid detection of resistant isolates.

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